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Journal of Assisted Reproduction and Genetics

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01-11-2011 | Epigenetics

Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos

Authors: Shahram Jafari, Sayyed Morteza Hosseini, Mehdi Hajian, Mohsen Forouzanfar, Farnoosh Jafarpour, Parvaneh Abedi, Somayyeh Ostadhosseini, Hasan Abbasi, Hamid Gourabi, Abdolhossein H. Shahverdi, Ahmad Dizaj Vosough, Maryam Anjomshoaa, Abd Wahid Haron, Norshariza Nordin, Halimatun Yaakub, Mohammad Hosein Nasr-Esfahani

Published in: Journal of Assisted Reproduction and Genetics | Issue 11/2011

Abstract

Purpose

To investigate the effect of epigenetic modification on pattern, time and capacity of transcription activation of POU5F1, the key marker of pluripotency, in cloned bovine embryos.

Methods

Bovine fibroblasts were stably transfected with POU5F1 promoter-driven enhanced green fluorescent protein (EGFP). This provided a visible marker to investigate the effect of post-activation treatment of cloned bovine embryos with trichostatin A (TSA) on time and capacity of POU5F1 expression and its subsequent effect on in vitro development of cloned bovine embryos.

Results

Irrespective of TSA treatment, POU5F1 expression was not detected until 8–16 cell stage, but was detected in both inner cell mass and trophectoderm at the blastocyst stage. TSA treatment significantly increased POU5F1 expression, and the yield and quality of cloned embryo development compared to control.

Conclusion

The POU5F1 expression of cloned embryos is strictly controlled by the stage of embryo development and may not be altered by TSA-mediated changes occur in DNA-methylation and histone-acetylation of the genome.

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Title: Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos
Authors: Shahram Jafari
Sayyed Morteza Hosseini
Mehdi Hajian
Mohsen Forouzanfar
Farnoosh Jafarpour
Parvaneh Abedi
Somayyeh Ostadhosseini
Hasan Abbasi
Hamid Gourabi
Abdolhossein H. Shahverdi
Ahmad Dizaj Vosough
Maryam Anjomshoaa
Abd Wahid Haron
Norshariza Nordin
Halimatun Yaakub
Mohammad Hosein Nasr-Esfahani
Publication date: 01-11-2011
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 11/2011
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9638-1

At a glance: The STEP trials

Springer Medicine

Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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