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International Journal of Colorectal Disease

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Open Access 01-01-2011 | Original Article

The plasminogen activator inhibitor system in colon cancer cell lines is influenced by the CO₂ pneumoperitoneum

Authors: Petra Krause, Nina S. Bobisch, Paul Thelen, Karola Koehler, Sarah Koenig, Heinz Becker, Ingo Leister

Published in: International Journal of Colorectal Disease | Issue 1/2011

Abstract

Purpose

Laparoscopic surgery in the treatment of colon carcinoma causes pH value alterations as well as changes in fibrinolytic activity. This results in enhanced proliferation of colon carcinoma cells in vitro and also in enhanced growth of liver metastasis when compared to isobaric (gasless) laparoscopy in vivo. So far, the direct influence of CO₂ pneumoperitoneum on the invasiveness and metastatic capabilities of colon cancer cells remains unclear. We therefore evaluated transcripts of the uPA system.

Methods

The influence of CO₂ pneumoperitoneum on the gene expression of plasminogen activator inhibitor-1 (PAI-1), urokinase-type plasminogen activator (uPA), and tissue-type plasminogen activator (tPA) was investigated in colon carcinoma cell lines (HT116, SW48, and WiDr) and mesothelial cells employing a pneumoperitoneum chamber in vitro. Quantitative gene expression data were collected using real-time RT-PCR and statistical analysis was performed by means of analysis of variance and Bonferroni correction.

Results

The expression of uPA and PAI-1 was increased in colon carcinoma cell lines when cultivated at pH 6.1, a value corresponding to intraabdominal pH values during CO₂ insufflation. Elevated PAI-1 mRNA levels were also observed when CO₂ was simultaneously applied with a pressure of 10 mmHg. In contrast, there were no significant changes in mesothelial cells in the investigated parameter.

Conclusion

The conditions of CO₂ pneumoperitoneum cause changes in the expression of genes controlling the fibrinolytic activity. The increase of PAI-1 and uPA can contribute to the enhancement of metastasis and invasive potential of tumour cells. Therefore, changes in the conditions of laparoscopy may well optimise laparoscopic therapy in colon cancer.

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Title: The plasminogen activator inhibitor system in colon cancer cell lines is influenced by the CO2 pneumoperitoneum
Authors: Petra Krause
Nina S. Bobisch
Paul Thelen
Karola Koehler
Sarah Koenig
Heinz Becker
Ingo Leister
Publication date: 01-01-2011
Publisher: Springer-Verlag
Published in: International Journal of Colorectal Disease / Issue 1/2011
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI: https://doi.org/10.1007/s00384-010-1062-y

Keynote webinar | Spotlight on medication adherence

Springer Medicine

The plasminogen activator inhibitor system in colon cancer cell lines is influenced by the CO₂ pneumoperitoneum

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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