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Archives of Virology
Published in: Archives of Virology 7/2009

01-07-2009 | Brief Report

The mouse Pol I terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like RNAs with precise 3′ ends in a plasmid-only-based virus rescue system

Authors: Liqiang Feng, Feng Li, Xuehua Zheng, Weiqi Pan, Kai Zhou, Yichu Liu, Hongxuan He, Ling Chen

Published in: Archives of Virology | Issue 7/2009

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Abstract

Reverse genetics systems for generating recombinant influenza viruses are based on two different mechanisms for obtaining the 3′ end of the viral RNA: one uses the self-cleaving hepatitis delta virus ribozyme (HDVR), and the other uses the murine RNA polymerase I (Pol I) terminator. In this study, we employed EGFP and Renilla luciferase reporter constructs to compare the efficiency of both methods. Our results indicate that the murine Pol I terminator was more efficient than the HDVR, which will be helpful in choosing an influenza virus rescue system, as well as in establishing other RNA virus rescue systems.
Literature
1.
Chen D, Barros M, Spencer E, Patton JT (2001) Features of the 3′-consensus sequence of rotavirus mRNAs critical to minus strand synthesis. Virology 282:221–229PubMedCrossRef
2.
Fodor E, Devenish L, Engelhardt OG, Palese P, Brownlee GG, Garcia-Sastre A (1999) Rescue of influenza A virus from recombinant DNA. J Virol 73:9679–9682PubMed
3.
Fujii K, Fujii Y, Noda T, Muramoto Y, Watanabe T, Takada A, Goto H, Horimoto T, Kawaoka Y (2005) Importance of both the coding and the segment-specific noncoding regions of the influenza A virus NS segment for its efficient incorporation into virions. J Virol 79:3766–3774PubMedCrossRef
4.
Fujii Y, Goto H, Watanabe T, Yoshida T, Kawaoka T (2003) Selective incorporation of influenza virus RNA segments into virions. Proc Natl Acad Sci USA 100:2002–2007PubMedCrossRef
5.
Hoffmann E, Neumann G, Hobom G, Webster RG, Kawaoka Y (2000) “Ambisense” approach for the generation of influenza A virus: vRNA and mRNA synthesis from one template. Virology 267:310–317PubMedCrossRef
6.
Hoffmann E, Neumann G, Kawaoka Y, Hobom G, Webster RG (2000) A DNA transfection system for generation of influenza A virus from eight plasmids. Proc Natl Acad Sci USA 97:6108–6113PubMedCrossRef
7.
Gerin JL, Casey JL, Purcell RH (2001) Hepatitis delta virus, chap 88, 4th edn. Williams & Wilkins, Baltimore
8.
Kuhn A, Grummt I (1989) 3′-end formation of mouse pre-rRNA involves both transcription termination and a specific processing reaction. Genes Dev 3:224–231PubMedCrossRef
9.
Liang Y, Hong Y, Parslow TG (2005) cis-Acting packaging signals in the influenza virus PB1, PB2, and PA genomic RNA segments. J Virol 79:10348–10355PubMedCrossRef
10.
Lutz A, Dyall J, Olivo PD, Pekosz A (2005) Virus-inducible reporter genes as a tool for detecting and quantifying influenza A virus replication. J Virol Methods 126:13–20PubMedCrossRef
11.
Marsh GA, Rabadan R, Levine AJ, Palese P (2008) Highly conserved regions of influenza a virus polymerase gene segments are critical for efficient viral RNA packaging. J Virol 82:2295–2304PubMedCrossRef
12.
Muramoto Y, Takada A, Fujii K, Noda T, Iwatsuki-Horimoto K, Watanabe S, Horimoto T, Kida H, Kawaoka Y (2006) Hierarchy among viral RNA (vRNA) segments in their role in vRNA incorporation into influenza A virions. J Virol 80:2318–2325PubMedCrossRef
13.
Neumann G, Watanabe T, Ito H, Watanabe S, Goto H, Gao P, Hughes M, Perez DR, Donis R, Hoffmann E, Hobom G, Kawaoka Y (1999) Generation of influenza A viruses entirely from cloned cDNAs. Proc Natl Acad Sci USA 96:9345–9350PubMedCrossRef
14.
Neumann G, Fujii K, Kino Y, Kawaoka Y (2005) An improved reverse genetics system for influenza A virus generation and its implications for vaccine production. Proc Natl Acad Sci USA 102:16825–16829PubMedCrossRef
15.
Ozawa M, Goto H, Horimoto T, Kawaoka Y (2007) An adenovirus vector-mediated reverse genetics system for influenza A virus generation. J Virol 81:9556–9559PubMedCrossRef
16.
Shih IH, Been MD (1999) Ribozyme cleavage of a 2, 5-phosphodiester linkage: mechanism and a restricted divalent metal-ion requirement. RNA 5:1140–1148PubMedCrossRef
17.
Tumpey TM, Basler CF, Aguilar PV, Zeng H, Solorzano A, Swayne DE, Cox NJ, Katz JM, Taubenberger JK, Palese P, Garcia-Sastre A (2005) Characterization of the reconstructed 1918 Spanish influenza pandemic virus. Science 310:77–80PubMedCrossRef
18.
Watanabe T, Watanabe S, Noda T, Fujii Y, Kawaoka Y (2003) Exploitation of nucleic acid packaging signals to generate a novel influenza virus-based vector stably expressing two foreign genes. J Virol 77:10575–10583PubMedCrossRef
19.
20.
de Wit E, Spronken MIJ, Bestebroer TM, Rimmelzwaan GF, Osterhaus ADME, Fouchier RAM (2004) Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments. Virus Res 103:155–161PubMedCrossRef
Metadata
Title
The mouse Pol I terminator is more efficient than the hepatitis delta virus ribozyme in generating influenza-virus-like RNAs with precise 3′ ends in a plasmid-only-based virus rescue system
Authors
Liqiang Feng
Feng Li
Xuehua Zheng
Weiqi Pan
Kai Zhou
Yichu Liu
Hongxuan He
Ling Chen
Publication date
01-07-2009
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 7/2009
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-009-0422-9

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