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01-05-2018 | Original Research Paper

The host control of a clinical isolate strain of P. aeruginosa infection is independent of Nod-1 but depends on MyD88

Authors: Fabiane Sônego, Fernanda V. S. Castanheira, Catarina V. Horta, Alexandre Kanashiro, Paula G. Czaikoski, Dario S. Zamboni, José Carlos Alves-Filho, Fernando Q. Cunha

Published in: Inflammation Research | Issue 5/2018

Abstract

Objective and design

The objective of this study was to investigate the role of Nod1 in the recruitment of neutrophils into the infection site and in the establishment of the inflammatory response elicited by a clinical isolate strain of P. aeruginosa in vivo, while comparing it to the well-established role of MyD88 in this process.

Subjects

Wild-type, Nod1^−/− and MyD88^−/− mice, all with a C57Bl/6 background.

Methods

Mice were intranasally infected with Pseudomonas aeruginosa DZ605. Bronchoalveolar lavage and blood were harvested 6 or 20 h post-infection for evaluating bacterial load, chemokine levels and neutrophil migration. Survival post-infection was also observed.

Results

We show here that wild-type and Nod1^−/− mice induce similar lung chemokine levels, neutrophil recruitment, and bacterial load, thus leading to equal survival rates upon P. aeruginosa pulmonary infection. Furthermore, we confirmed the essential role of MyD88-dependent signalling in recruiting neutrophils and controlling P. aeruginosa-induced pulmonary infection.

Conclusion

The results suggest that in contrast to MyD88, under our experimental conditions, the absence of Nod1 does not impair the recruitment of neutrophils in response to P. aeruginosa DZ605.

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Title: The host control of a clinical isolate strain of P. aeruginosa infection is independent of Nod-1 but depends on MyD88
Authors: Fabiane Sônego
Fernanda V. S. Castanheira
Catarina V. Horta
Alexandre Kanashiro
Paula G. Czaikoski
Dario S. Zamboni
José Carlos Alves-Filho
Fernando Q. Cunha
Publication date: 01-05-2018
Publisher: Springer International Publishing
Published in: Inflammation Research / Issue 5/2018
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-018-1135-x

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Methods

Results

Conclusion

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