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Strahlentherapie und Onkologie
Published in: Strahlentherapie und Onkologie 1/2014

01-01-2014 | Original article

The effect of resveratrol in combination with irradiation and chemotherapy

Study using Merkel cell carcinoma cell lines

Authors: G. Heiduschka, C. Lill, R. Seemann, M. Brunner, R. Schmid, R. Houben, J. Bigenzahn, D. Thurnher, M.D.

Published in: Strahlentherapie und Onkologie | Issue 1/2014

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Abstract

Background and purpose

Merkel cell carcinoma (MCC) is a rare, but highly malignant tumor of the skin. In case of systemic disease, possible therapeutic options include irradiation or chemotherapy. The aim of this study was to evaluate whether the flavonoid resveratrol enhances the effect of radiotherapy or chemotherapy in MCC cell lines.

Materials and methods

The two MCC cell lines MCC13 and MCC26 were treated with increasing doses of resveratrol. Combination experiments were conducted with cisplatin and etoposide. Colony forming assays were performed after sequential irradiation with 1, 2, 3, 4, 6, and 8 Gy and apoptosis was assessed with flow cytometry. Expression of cancer drug targets was analyzed by real-time PCR array.

Results

Resveratrol is cytotoxic in MCC cell lines. Cell growth is inhibited by induction of apoptosis. The combination with cisplatin and etoposide resulted in a partially synergistic inhibition of cell proliferation. Resveratrol and irradiation led to a synergistic reduction in colony formation compared to irradiation alone. Evaluation of gene expression did not show significant difference between the cell lines.

Conclusion

Due to its radiosensitizing effect, resveratrol seems to be a promising agent in combination with radiation therapy. The amount of chemosensitizing depends on the cell lines tested.
Literature
1.
Becker JC (2010) Merkel cell carcinoma. Ann Oncol 21(Suppl 7):vii81–vii85PubMed
2.
3.
Bickle K, Glass LF, Messina JL et al (2004) Merkel cell carcinoma: a clinical, histopathologic, and immunohistochemical review. Semin Cutan Med Surg 23(1):46–53PubMedCrossRef
4.
Poulsen M (2005) Merkel cell carcinoma of skin: diagnosis and management strategies. Drugs Aging 22(3):219–229PubMedCrossRef
5.
Albores-Saavedra J, Batich K, Chable-Montero F et al (2010) Merkel cell carcinoma demographics, morphology, and survival based on 3870 cases: a population based study. J Cutan Pathol 37(1):20–27PubMedCrossRef
6.
Calder KB, Smoller BR (2010) New insights into Merkel cell carcinoma. Adv Anat Pathol 17(3):155–161PubMedCrossRef
7.
Bischof M, Kampen M van, Huber P, Wannenmacher M (1999) Merkel cell carcinoma: the role of radiation therapy in general management. Strahlenther Onkol 175(12):611–615PubMedCrossRef
8.
Roeder F, Krempien R, Sterzing F et al (2007) Radiotherapy for localised and advanced Merkel cell carcinoma of the skin: a single institution case series. Eur J Dermatol 17(3):229–233PubMed
9.
Dancey AL, Rayatt SS, Soon C et al (2006) Merkel cell carcinoma: a report of 34 cases and literature review. J Plast Reconstr Aesthet Surg 59(12):1294–1299PubMedCrossRef
10.
Sas-Korczyńska B, Korzeniowski S, Wójcik E (2010) Comparison of the effectiveness of “late” and “early” prophylactic cranial irradiation in patients with limited-stage small cell lung cancer. Strahlenther Onkol 186(6):315–319PubMedCrossRef
11.
Baumgart T, Klautke G, Kriesen S et al (2012) Radiosensitizing effect of epothilone B on human epithelial cancer cells. Strahlenther Onkol 188(2):177–184PubMedCrossRef
12.
Stingl L, Niewidok N, Müller N et al (2012) Radiosensitizing effect of the novel Hsp90 inhibitor NVP-AUY922 in human tumour cell lines silenced for Hsp90α. Strahlenther Onkol 188(6):507–515PubMedCrossRef
13.
Kotowski U, Heiduschka G, Brunner M et al (2011) Radiosensitization of head and neck cancer cells by the phytochemical agent sulforaphane. Strahlenther Onkol 187(9):575–580PubMedCrossRef
14.
Takagi M, Sakata K, Someya M et al (2012) The combination of hyperthermia or chemotherapy with gimeracil for effective radiosensitization. Strahlenther Onkol 188(3):255–261PubMedCrossRef
15.
Johnson GE, Ivanov VN, Hei TK (2008) Radiosensitization of melanoma cells through combined inhibition of protein regulators of cell survival. Apoptosis 13(6):790–802PubMedCrossRef
16.
Aluyen JK, Ton QN, Tran T et al (2012) Resveratrol: potential as anticancer agent. J Diet Suppl 9(1):45–56PubMedCrossRef
17.
Knutson MD, Leeuwenburgh C (2008) Resveratrol and novel potent activators of SIRT1: effects on aging and age-related diseases. Nutr Rev 66(10):591–596PubMedCrossRef
18.
Vang O, Ahmad N, Baile CA et al (2011) What is new for an old molecule? Systematic review and recommendations on the use of resveratrol. PLoS One 6(6):e19881PubMedCentralPubMedCrossRef
19.
Heiduschka G, Erovic BM, Vormittag L et al (2009) 7beta-hydroxycholesterol induces apoptosis and regulates cyclooxygenase 2 in head and neck squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 135(3):261–267PubMedCrossRef
20.
Chou TC, Talalay P (1984) Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors. Adv Enzyme Regul 22:27–55PubMedCrossRef
21.
Franken NAP, Rodermond HM, Stap J et al (2006) Clonogenic assay of cells in vitro. Nat Protoc 1(5):2315–2319PubMedCrossRef
22.
Eder-Czembirek C, Erovic BM, Czembirek C et al (2010) Betulinic acid a radiosensitizer in head and neck squamous cell carcinoma cell lines. Strahlenther Onkol 186(3):143–148PubMedCrossRef
23.
R Development Core Team 2 (2013) R: a language and environment for statistical computing. Vienna(Austria):ISBN3-900051-07-0-URLhttp:-www.R- project.org
24.
Fournier DA, Skaug HJ, Ancheta J, Ianelli J (2012) AD Model Builder: using automatic differentiation for statistical inference of highly parameterized complex nonlinear models. Optim Method …
25.
Skaug HJ, Fournier DA, Nielsen A et al (o J) Generalized linear mixed models using ad model builder. version 0.7.2.12. URL http:r-forge.r-project.org/projects/glmmadmb/. Accessed 01 Oct 2013
26.
Duprat JP, Landman G, Salvajoli JV, Brechtbühl ER (2011) A review of the epidemiology and treatment of Merkel cell carcinoma. Clinics 66(10):1817–1823PubMedCentralPubMedCrossRef
27.
Vergara D, Simeone P, Toraldo D et al (2012) Resveratrol downregulates Akt/GSK and ERK signalling pathways in OVCAR-3 ovarian cancer cells. Mol Biosyst 8(4):1078–1087PubMedCrossRef
28.
Miki H, Uehara N, Kimura A et al (2012) Resveratrol induces apoptosis via ROS-triggered autophagy in human colon cancer cells. Int J Oncol 40(4):1020–1028PubMedCentralPubMed
29.
Kartal M, Saydam G, Sahin F, Baran Y (2011) Resveratrol triggers apoptosis through regulating ceramide metabolizing genes in human K562 chronic myeloid leukemia cells. Nutr Cancer 63(4):637–644PubMedCrossRef
30.
Xie Y, Zhang J, Xu Y, Shao C (2012) SirT1 confers hypoxia-induced radioresistance via the modulation of c-Myc stabilization on hepatoma cells. J Radiat Res 53(1):44–50PubMedCrossRef
31.
Casanova F, Quarti J, Costa DCF da et al (2012) Resveratrol chemosensitizes breast cancer cells to melphalan by cell cycle arrest. J Cell Biochem n/a–n/a
32.
Santandreu FM, Valle A, Oliver J, Roca P (2011) Resveratrol potentiates the cytotoxic oxidative stress induced by chemotherapy in human colon cancer cells. Cell Physiol Biochem 28(2):219–228PubMedCrossRef
Metadata
Title
The effect of resveratrol in combination with irradiation and chemotherapy
Study using Merkel cell carcinoma cell lines
Authors
G. Heiduschka
C. Lill
R. Seemann
M. Brunner
R. Schmid
R. Houben
J. Bigenzahn
D. Thurnher, M.D.
Publication date
01-01-2014
Publisher
Springer Berlin Heidelberg
Published in
Strahlentherapie und Onkologie / Issue 1/2014
Print ISSN: 0179-7158
Electronic ISSN: 1439-099X
DOI
https://doi.org/10.1007/s00066-013-0445-8

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