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01-02-2011 | Original Article

The effect of lentivirus-mediated TH and GDNF genetic engineering mesenchymal stem cells on Parkinson’s disease rat model

Authors: Dezhi Shi, Gong Chen, Li Lv, Lixin Li, Dong Wei, Peiyuan Gu, Juemin Gao, Yi Miao, Weixing Hu

Published in: Neurological Sciences | Issue 1/2011

Abstract

This study was designed to assess the potential therapeutic efficacy of gene-modified mesenchymal stem cells (MSCs), MSCs-TH and MSCs-GDNF, in PD rats. Fifty-nine PD rat models were divided into five groups and then the gene-modified MSCs were transplanted into the striatum of rats according to the design. Apomorphine-induced rotational behavior in rats was observed weekly; rats which received both MSCs-TH and MSCs-GDNF showed the most significant improvement compared with those in other groups (P < 0.01). Three weeks later, immunohistochemistry analysis found TH-positive cells and GDNF-positive cells in striatal. Eight weeks later, PD rats were killed. HPLC and ELISA results showed DA and GDNF content in striatum of rats which received both MSCs-TH, and MSCs-GDNF was considerably higher compared with those of other groups (P < 0.01),respectively. In conclusion, our results suggest that combined transplantation of MSCs expressing TH and GDNF can lead to remarkable therapeutic effects in a rat model of PD.

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Title: The effect of lentivirus-mediated TH and GDNF genetic engineering mesenchymal stem cells on Parkinson’s disease rat model
Authors: Dezhi Shi
Gong Chen
Li Lv
Lixin Li
Dong Wei
Peiyuan Gu
Juemin Gao
Yi Miao
Weixing Hu
Publication date: 01-02-2011
Publisher: Springer Milan
Published in: Neurological Sciences / Issue 1/2011
Print ISSN: 1590-1874
Electronic ISSN: 1590-3478
DOI: https://doi.org/10.1007/s10072-010-0385-3

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The effect of lentivirus-mediated TH and GDNF genetic engineering mesenchymal stem cells on Parkinson’s disease rat model

Abstract

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Abstract

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