Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
Maxillofacial Plastic and Reconstructive Surgery
Published in: Maxillofacial Plastic and Reconstructive Surgery 1/2017

Open Access 01-12-2017 | Research

The effect of biomechanical stimulation on osteoblast differentiation of human jaw periosteum-derived stem cells

Authors: Ju-Min Lee, Min-Gu Kim, June-Ho Byun, Gyoo-Cheon Kim, Jung-Hoon Ro, Dae-Seok Hwang, Byul-Bora Choi, Geun-Chul Park, Uk-Kyu Kim

Published in: Maxillofacial Plastic and Reconstructive Surgery | Issue 1/2017

Login to get access

Abstract

Background

This study was to investigate the effect of biomechanical stimulation on osteoblast differentiation of human periosteal-derived stem cell using the newly developed bioreactor.

Methods

Human periosteal-derived stem cells were harvested from the mandible during the extraction of an impacted third molar. Using the new bioreactor, 4% cyclic equibiaxial tension force (0.5 Hz) was applied for 2 and 8 h on the stem cells and cultured for 3, 7, and 14 days on the osteogenic medium. Biochemical changes of the osteoblasts after the biomechanical stimulation were investigated. No treatment group was referred to as control group.

Results

Alkaline phosphatase (ALP) activity and ALP messenger RNA (mRNA) expression level were higher in the strain group than those in the control group. The osteocalcin and osteonectin mRNA expressions were higher in the strain group compared to those in the control group on days 7 and 14. The vascular endothelial growth factor (VEGF) mRNA expression was higher in the strain group in comparison to that in the control group. Concentration of alizarin red S corresponding to calcium content was higher in the strain group than in the control group.

Conclusions

The study suggests that cyclic tension force could influence the osteoblast differentiation of periosteal-derived stem cells under optimal stimulation condition and the force could be applicable for tissue engineering.
Literature
1.
Ilizarov G (1971) Basic principles of transosseous compression and distraction osteosynthesis. Ortopediia travmatologiia i protezirovanie 32:7–15PubMed
2.
Ilizarov GA (1989) The tension-stress effect on the genesis and growth of tissues: Part I. The influence of stability of fixation and soft-tissue preservation. Clin Orthop Relat Res 238:249–281
3.
Ignatius A, Blessing H, Liedert A, Schmidt C, Neidlinger-Wilke C, Kaspar D et al (2005) Tissue engineering of bone: effects of mechanical strain on osteoblastic cells in type I collagen matrices. Biomaterials 26:311–318CrossRefPubMed
4.
Tanaka SM, Li J, Duncan RL, Yokota H, Burr DB, Turner CH (2003) Effects of broad frequency vibration on cultured osteoblasts. J Biomech 36:73–80CrossRefPubMed
5.
Mofid MM, Manson PN, Robertson BC, Tufaro AP, Elias JJ, Vander Kolk CA et al (2001) Craniofacial distraction osteogenesis: a review of 3278 cases. Plast Reconstr Surg 108:1103–1114CrossRefPubMed
6.
Garcia AG, Martin MS, Vila PG, Maceiras JL (2002) Minor complications arising in alveolar distraction osteogenesis. J Oral Maxillofac Surg 60:496–501CrossRefPubMed
7.
Kim U-K, Chung I-K, Lee K-H, Swift JQ, Seong W-J, Ko C-C (2006) Bone regeneration in mandibular distraction osteogenesis combined with compression stimulation. J Oral Maxillofac Surg 64:1498–1505CrossRefPubMed
8.
Kim U-K, Park S-J, Seong W-J, Heo J, Hwang D-S, Kim Y-D et al (2010) Expression of TGF-β1, osteonectin, and BMP-4 in mandibular distraction osteogenesis with compression stimulation: reverse transcriptase-polymerase chain reaction study and biomechanical test. J Oral Maxillofac Surg 68:2076–2084CrossRefPubMed
9.
Hamilton DW, Maul TM, Vorp DA (2004) Characterization of the response of bone marrow-derived progenitor cells to cyclic strain: implications for vascular tissue-engineering applications. Tissue Eng 10:361–369CrossRefPubMed
10.
Matheson LA, Maksym GN, Santerre JP, Labow RS (2007) Differential effects of uniaxial and biaxial strain on U937 macrophage-like cell morphology: influence of extracellular matrix type proteins. J Biomed Mater Res Part A 81:971–981CrossRef
11.
Kim I, Song Y, Lee B, Hwang S (2012) Human mesenchymal stromal cells are mechanosensitive to vibration stimuli. J Dent Res 91:1135–1140CrossRefPubMed
12.
Wu Y, Zhang X, Zhang P, Fang B, Jiang L (2012) Intermittent traction stretch promotes the osteoblastic differentiation of bone mesenchymal stem cells by the ERK1/2-activated Cbfa1 pathway. Connect Tissue Res 53:451–459CrossRefPubMed
13.
Breitbart AS, Grande DA, Kessler R, Ryaby JT, Fitzsimmons RJ, Grant RT (1998) Tissue engineered bone repair of calvarial defects using cultured periosteal cells. Plast Reconstr Surg 101:567–574CrossRefPubMed
14.
Hutmacher DW, Sittinger M (2003) Periosteal cells in bone tissue engineering. Tissue Eng 9:45–64CrossRef
15.
Bhatt KA, Chang EI, Warren SM, Lin S-e, Bastidas N, Ghali S et al (2007) Uniaxial mechanical strain: an in vitro correlate to distraction osteogenesis. J Surg Res 143:329–336CrossRefPubMed
16.
Hara F, Fukuda K, Ueno M, Hamanishi C, Tanaka S (1999) Pertussis toxin-sensitive G proteins as mediators of stretch-induced decrease in nitric-oxide release of osteoblast-like cells. J Orthop Res 17:593–597CrossRefPubMed
17.
Choi S, Kim J, Kang E-J, Lee S-W, Park M-C, Park Y-B et al (2008) Osteopontin might be involved in bone remodelling rather than in inflammation in ankylosing spondylitis. Rheumatology 47:1775–1779CrossRefPubMed
18.
Park B-W, Hah Y-S, Kim DR, Kim J-R, Byun J-H (2007) Osteogenic phenotypes and mineralization of cultured human periosteal-derived cells. Arch Oral Biol 52:983–989CrossRefPubMed
19.
Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP (1997) Osteogenic differentiation of purified, culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem 64:295–312CrossRefPubMed
20.
Kotobuki N, Hirose M, Machida H, Katou Y, Muraki K, Takakura Y et al (2005) Viability and osteogenic potential of cryopreserved human bone marrow-derived mesenchymal cells. Tissue Eng 11:663–673CrossRefPubMed
21.
Park B-W, Hah Y-S, Kim DR, Kim J-R, Byun J-H (2008) Vascular endothelial growth factor expression in cultured periosteal-derived cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 105:554–560CrossRefPubMed
22.
Hale L, Ma Y, Santerre R (2000) Semi-quantitative fluorescence analysis of calcein binding as a measurement of in vitro mineralization. Calcif Tissue Int 67:80–84CrossRefPubMed
23.
Anselme K, Broux O, Noel B, Bouxin B, Bascoulergue G, Dudermel A-F et al (2002) In vitro control of human bone marrow stromal cells for bone tissue engineering. Tissue Eng 8:941–953CrossRefPubMed
24.
Kim H, Iwasaki K, Miyake T, Shiozawa T, Nozaki S, Yajima K (2003) Changes in bone turnover markers during 14-day 6 head-down bed rest. J Bone Miner Metab 21:311–315CrossRefPubMed
25.
Deckers MM, Karperien M, van der Bent C, Yamashita T, Papapoulos SE, Löwik CW (2000) Expression of vascular endothelial growth factors and their receptors during osteoblast differentiation. Endocrinology 141:1667–1674CrossRef
Metadata
Title
The effect of biomechanical stimulation on osteoblast differentiation of human jaw periosteum-derived stem cells
Authors
Ju-Min Lee
Min-Gu Kim
June-Ho Byun
Gyoo-Cheon Kim
Jung-Hoon Ro
Dae-Seok Hwang
Byul-Bora Choi
Geun-Chul Park
Uk-Kyu Kim
Publication date
01-12-2017
Publisher
Springer Berlin Heidelberg
Published in
Maxillofacial Plastic and Reconstructive Surgery / Issue 1/2017
Electronic ISSN: 2288-8586
DOI
https://doi.org/10.1186/s40902-017-0104-6

Other articles of this Issue 1/2017

Maxillofacial Plastic and Reconstructive Surgery 1/2017 Go to the issue

Review

Treatment of dental implant displacement into the maxillary sinus

Case report

Flap necrosis after palatoplasty in irradiated patient and its reconstruction with tunnelized-facial artery myomucosal island flap

Research

Comparison of the effects on the pharyngeal airway space of maxillary protraction appliances according to the methods of anchorage

Research

Usefulness and capability of three-dimensional, full high-definition movies for surgical education

Research

The comparative study of resonance disorders for Vietnamese and Korean cleft palate speakers using nasometer

Research

Evaluation of bone healing using rhBMP-2 soaked hydroxyapatite in ridge augmentation: a prospective observational study