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Open Access 01-12-2024 | Tamoxifen | Research

Cre-LoxP and tamoxifen-induced deletion of ovarian quiescin sulfhydryl oxidase 2 showed disruption of ovulatory activity in mice

Authors: Shih-Yun Chen, Tse-En Wang, Wei-Yun Lee, Ya-Yi Yang, Hong-Chun Lai, Fuko Matsuda, Haruhiko Kosek, You-Tzung Chen, Sheng-Hsiang Li, Pei-Shiue Tsai

Published in: Journal of Ovarian Research | Issue 1/2024

Abstract

Background

Quiescin sulfhydryl oxidase 2 (QSOX2) is a flavin adenine dinucleotide-dependent sulfhydryl oxidase that is known to be involved in protein folding, cell growth regulation, and redox state modification through oxidative activities. Earlier studies demonstrated the tissue and cellular localization of QSOX2 in the male reproductive tract, as well as the highly-regulated mechanism of QSOX2 protein synthesis and expression through the coordinated action of testosterone and epididymal-enriched amino acid, glutamate. However, the presence and the functions of QSOX2 in female reproduction are unknown. In this study, we applied the Cre-loxP gene manipulation system to generate the heterozygous and homozygous Qsox2 knockout mice and examined its effects on ovarian function.

Results

We demonstrated that QSOX2 was detected in the follicle-supporting cells (granulosa and cumulus cells) of ovarian follicles of all stages but was absent in the corpus luteum, suggesting its supportive role in folliculogenesis. In comparison with reproductive organogenesis in wild-type mice, there was no difference in testicular and epididymal structure in male Qsox2 knockout; however, Qsox2 knockout disrupted the regular ovulation process in female mice as a drastic decrease in the formation of the corpus luteum was detected, and no pregnancy was achieved when mating males with homozygous Qsox2 knockout females. RNAseq analyses further revealed that Qsox2 knockout altered critical signaling pathways and genes that are responsible for maintaining ovarian functions.

Conclusion

Our data demonstrated for the first time that Qsox2 is critical for ovarian function in mice.

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Title: Cre-LoxP and tamoxifen-induced deletion of ovarian quiescin sulfhydryl oxidase 2 showed disruption of ovulatory activity in mice
Authors: Shih-Yun Chen
Tse-En Wang
Wei-Yun Lee
Ya-Yi Yang
Hong-Chun Lai
Fuko Matsuda
Haruhiko Kosek
You-Tzung Chen
Sheng-Hsiang Li
Pei-Shiue Tsai
Publication date: 01-12-2024
Publisher: BioMed Central
Keywords: Tamoxifen
Estrogens
Published in: Journal of Ovarian Research / Issue 1/2024
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/s13048-024-01388-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Cre-LoxP and tamoxifen-induced deletion of ovarian quiescin sulfhydryl oxidase 2 showed disruption of ovulatory activity in mice

Abstract

Background

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Results

Conclusion

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