Published in:
01-03-2009 | Original Paper
Study on relationship between angiogenesis and micrometastases of peripheral blood in breast cancer
Authors:
Xiao-Dong Xie, Shu-Xian Qu, Zhao-Zhe Liu, Fang Zhang, Zhen-Dong Zheng
Published in:
Journal of Cancer Research and Clinical Oncology
|
Issue 3/2009
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Abstract
Purpose
To investigate the relationship between microvessel density, expression of vascular endothelial growth factor A (VEGF-A) and micrometastases in peripheral blood of patients with breast cancer.
Method
Microvessel density (MVD) and expression of VEGF-A were detected by immunohistochemistry S-P. Nested RT-PCR was introduced to detect the expression of hMAM mRNA in peripheral blood of all cases.
Result
Average MVD was 28.95 ± 6.95 microvessels/100× and positive rate of VEGF-A was 64.0% (32/50) in 50 cases with breast cancer. MVD count and expression of VEGF-A were related to tumor size, metastasis of axillary lymph nodes and clinical stages (P < 0.05), independent of age and histological classification (P > 0.05). The positive rate of hMAM mRNA in peripheral blood was 34.0% (17/50), which correlated with lymphatic metastasis and clinical stages (P < 0.05), independent of pathological category, menopause and hormone receptor (P > 0.05). MVD count and positive rate of VEGF-A in breast cancer with positive expression of hMAM mRNA was obviously higher than those without hMAM mRNA expression (χ
2 = 5.766, P = 0.032; t = 5.37, P = 0.002).
Conclusions
MVD count and positive expression of VEGF-A closely correlated to hMAM mRNA released from tumor cells in the circulation. hMAM mRNA is expected to become a valuable marker for further study on micrometastases of breast cancer.