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European Journal of Clinical Microbiology & Infectious Diseases
Published in: European Journal of Clinical Microbiology & Infectious Diseases 7/2021

01-07-2021 | Streptococci | Brief Report

Development and standardization of a specific real-time PCR assay for the rapid detection of Candida auris

Authors: Ahmad Ibrahim, Sophie Alexandra Baron, Hanane Yousfi, Linda Hadjadj, Rym Lalaoui, Serge Morand, Jean-Marc Rolain, Fadi Bittar

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 7/2021

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Abstract

Candida auris is an emerging multiresistant pathogen causing nosocomial fungal infection. Specific detection and identification are necessary. Our goal is to develop a new qPCR system that enables rapid detection of C. auris, based on a GPI (glycosyl-phosphatidylinositol) protein-encoding gene. This system is reproducible and sensitive with a limit of detection of 13 C. auris CFU/qPCR reaction. The 100% specificity of this system is confirmed on 2073 clinical and environmental samples, 50 different bacterial species, and 9 Candida spp. (70 strains). This system is suitable to correctly identify C. auris infections and to trace its source.
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Metadata
Title
Development and standardization of a specific real-time PCR assay for the rapid detection of Candida auris
Authors
Ahmad Ibrahim
Sophie Alexandra Baron
Hanane Yousfi
Linda Hadjadj
Rym Lalaoui
Serge Morand
Jean-Marc Rolain
Fadi Bittar
Publication date
01-07-2021
Publisher
Springer Berlin Heidelberg
Keyword
Streptococci
Published in
European Journal of Clinical Microbiology & Infectious Diseases / Issue 7/2021
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI
https://doi.org/10.1007/s10096-021-04176-8

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