Storage related haematological and biochemical changes in Plasmodium falciparum infected and sickle cell trait donor blood

In sub-Saharan Africa where sickle cell trait (SCT) and malaria is prevalent, significant proportions of blood donors may be affected by one or more of these abnormalities. The haemato-biochemical properties of SCT and asymptomatic malaria in donor blood have not been evaluated. This study evaluated the haemato-biochemical impact of SCT and asymptomatic malaria infections in citrate-phosphate-dextrose-adenine (CPDA-1) stored donor blood units.

Storage of SCT and malaria infected blood affected all haematological cell lines. In the SCT donors, red blood cells (RBC) (4.75 × 10¹²/L ± 1.43^baseline to 3.49 × 10¹²/L ± 1.09^week-5), haemoglobin (14.45 g/dl ± 1.63^baseline to 11.43 g/dl ± 1.69^week-5) and haematocrit (39.96% ± 3.18^baseline to 33.22% ± 4.12^week-5) were reduced. In the asymptomatic malaria group, reductions were observed in RBC (5.00 × 10¹²/L ± 0.75^baseline to 3.72 × 10¹²/L ± 0.71^week-5), haemoglobin (14.73 g/dl ± 1.67^baseline to 11.53 g/dl ± 1.62^week-5), haematocrit (42.72% ± 5.16^baseline to 33.38% ± 5.80^week-5), mean cell haemoglobin concentration (35.48 g/dl ± 1.84^baseline to 35.01 g/dl ± 0.64^week-5) and red cell distribution width coefficient of variation (14.81% ± 1.54^baseline to 16.26% ± 1.37^week-5). Biochemically, whereas plasma LDH levels significantly increased in asymptomatic malaria blood donors (319% increase at week 5 compared to baseline), SCT blood donors had the most significant increase in plasma potassium levels at week 5 (382% increase). Sodium ions significantly reduced in SCT/malaria negative and sickle cell trait blood at an average rate of 0.21 mmol/L per day. Moreover, elevations in lymphocytes-to-eosinophils and lymphocytes-to-neutrophils ratios were associated with SCT and malaria positive blood whilst elevation lymphocytes-to-basophils ratio was exclusive to malaria positive blood.

Severe storage lesions were significant in SCT or malaria positive donor blood units. Proper clinical evaluation must be done in prospective blood donors to ensure deferral of such donors.