Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Archives of Virology
Published in: Archives of Virology 8/2016

01-08-2016 | Brief Report

Soil-borne wheat mosaic virus infectious clone and manipulation for gene-carrying capacity

Authors: Sridhar Jarugula, Steven R. Charlesworth, Feng Qu, Lucy R. Stewart

Published in: Archives of Virology | Issue 8/2016

Login to get access

Abstract

A full-length infectious cDNA clone of soil-borne wheat mosaic virus (SBWMV; genus Furovirus; family Virgaviridae) was developed for agrobacterium delivery. The cloned virus can be agroinfiltrated to Nicotiana benthamiana for subsequent infection of wheat (Triticum aestivum, L.). The utility of the virus as a vector for gene silencing and expression was assessed through sequence insertions in multiple sites of RNA2. Virus-induced photobleaching was observed in N. benthamiana but not in wheat, despite the stability of the inserts. The SBWMV infectious clone can be used for further studies to investigate the biology of SBWMV through mutagenesis.
Literature
1.
Benavente LM, Ding XS, Redinbaugh MG, Nelson RS, Balint-Kurti P (2012) Virus-induced gene silencing in diverse maize lines using the brome mosaic virus-based silencing vector. Maydica 57:206–214
2.
Bruun-Rasmussen M, Madsen CT, Jessing S, Albrechtsen M (2007) Stability of barley stripe mosaic virus-induced gene silencing in barley. Mol Plant Microbe Interact 20:1323–1331CrossRefPubMed
3.
Chen JP, Macfarlane SA, Wilson TMA (1995) An analysis of spontaneous deletion sites in soil-borne wheat mosaic-virus Rna2. Virology 209:213–217CrossRefPubMed
4.
Crameri A, Whitehorn EA, Tate E, Stemmer WP (1996) Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat Biotechnol 14:315–319CrossRefPubMed
5.
Davies C, Haseloff J, Symons RH (1990) Structure, self-cleavage, and replication of two viroid-like satellite RNAs (virusoids) of subterranean clover mottle virus. Virology 177:216–224CrossRefPubMed
6.
Ding XS, Schneider WL, Chaluvadi SR, Mian MAR, Nelson RS (2006) Characterization of a Brome mosaic virus strain and its use as a vector for gene silencing in monocotyledonous hosts. Mol Plant Microbe Interact 19:1229–1239CrossRefPubMed
7.
Ding XS, Rao CS, Nelson RS (2007) Analysis of gene function in rice through virus-induced gene silencing. Methods Mol Biol 354:145–160PubMed
8.
Estes AP, Brakke MK (1966) Correlation of Polymyxa graminis with transmission of soil-borne wheat mosaic virus. Virology 28:772–774CrossRefPubMed
9.
Gibson DG, Young L, Chuang R-Y, Venter JC, Hutchison CA, Smith HO (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods 6:343–345CrossRefPubMed
10.
Gopinath K, Wellink J, Porta C, Taylor KM, Lomonossoff GP, van Kammen A (2000) Engineering cowpea mosaic virus RNA-2 into a vector to express heterologous proteins in plants. Virology 267:159–173CrossRefPubMed
11.
Gowda S, Satyanarayana T, Robertson C, Garnsey S, Dawson W (2005) Infection of citrus plants with virions generated in Nicotiana benthamiana plants agroinfiltrated with a binary vector based Citrus tristeza virus. In: Proceedings of the 16th conference of the international organization of citrus virologists. IOCV, Riverside, pp 23–33
12.
Kuhne T (2009) Soil-borne viruses affecting cereals—known for long but still a threat. Virus Res 141:174–183CrossRefPubMed
13.
Miyanishi M, Roh SH, Yamamiya A, Ohsato S, Shirako Y (2002) Reassortment between genetically distinct Japanese and US strains of Soil-borne wheat mosaic virus: RNA1 from a Japanese strain and RNA2 from a US strain make a pseudorecombinant virus. Arch Virol 147:1141–1153CrossRefPubMed
14.
Ohsato S, Miyanishi M, Shirako Y (2003) The optimal temperature for RNA replication in cells infected by soil-borne wheat mosaic virus is 17 °C. J Gen Virol 84:995–1000CrossRefPubMed
15.
Pacak A, Geisler K, Jorgensen B, Barciszewska-Pacak M, Nilsson L, Nielsen TH, Johansen E, Gronlund M, Jakobsen I, Albrechtsen M (2010) Investigations of barley stripe mosaic virus as a gene silencing vector in barley roots and in Brachypodium distachyon and oat. Plant Methods 6:26CrossRefPubMedPubMedCentral
16.
Scholthof HB (2006) The Tombusvirus-encoded P19: from irrelevance to elegance. Nat Rev Microbiol 4:405–411CrossRefPubMed
17.
Scofield SR, Huang L, Brandt AS, Gill BS (2005) Development of a virus-induced gene-silencing system for hexaploid wheat and its use in functional analysis of the Lr21-mediated leaf rust resistance pathway. Plant Physiol 138:2165–2173CrossRefPubMedPubMedCentral
18.
Shirako Y, Brakke MK (1984) 2 purified RNAs of soil-borne wheat mosaic-virus are needed for infection. J Gen Virol 65:119–127CrossRef
19.
Shirako Y, Wilson TMA (1993) Complete nucleotide-sequence and organization of the bipartite RNA genome of soil-borne wheat mosaic-virus. Virology 195:16–32CrossRefPubMed
20.
Stewart LR, Paul PA, Qu F, Redinbaugh MG, Miao H, Todd J, Jones M (2013) Wheat mosaic virus (WMoV), the causal agent of high plains disease, is present in Ohio wheat fields. Plant Dis 97:1125CrossRef
21.
Yamamiya A, Shirako Y (2000) Construction of full-length cDNA clones to Soil-borne wheat mosaic virus RNA1 and RNA2, from which infectious RNAs are transcribed in vitro: virion formation and systemic infection without expression of the N-terminal and C-terminal extensions to the capsid protein. Virology 277:66–75CrossRefPubMed
22.
Yamamiya A, Miyanishi M, Shirako Y (2005) Stable deletions arising in the readthrough region of Soil-borne wheat mosaic virus RNA2 define the 5′ limit of the functional promoter for the p19 subgenomic RNA. Arch Virol 150:1871–1884CrossRefPubMed
23.
Yuan C, Li C, Yan L, Jackson AO, Liu Z, Han C, Yu J, Li D (2011) A high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots. PLoS One 6:e26468CrossRefPubMedPubMedCentral
Metadata
Title
Soil-borne wheat mosaic virus infectious clone and manipulation for gene-carrying capacity
Authors
Sridhar Jarugula
Steven R. Charlesworth
Feng Qu
Lucy R. Stewart
Publication date
01-08-2016
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 8/2016
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-016-2863-2

Other articles of this Issue 8/2016

Archives of Virology 8/2016 Go to the issue

Original Article

Hepatitis C virus and its protein NS4B activate the cancer-related STAT3 pathway via the endoplasmic reticulum overload response

Original Article

Insect-specific flaviviruses in Aedes mosquitoes in Greece

Original Article

Isolation and molecular characterization of porcine epidemic diarrhea viruses collected in Japan in 2014

Review

Beak and feather disease virus in wild and captive parrots: an analysis of geographic and taxonomic distribution and methodological trends

Original Article

Screening of human bocavirus in surgically excised cancer specimens

Original Article

Coxsackievirus B3 infection induces autophagic flux, and autophagosomes are critical for efficient viral replication
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits