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Archives of Gynecology and Obstetrics

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01-04-2013 | Reproductive Medicine

Small GTPases are involved in sprout formation in human granulosa lutein cells

Authors: Maximilian B. Franz, Stefanie Daube, Christoph Keck, Michael Sator, Detlef Pietrowski

Published in: Archives of Gynecology and Obstetrics | Issue 4/2013

Abstract

Introduction

The corpus luteum (CL), develops from the ruptured follicle after gonadotropin stimulation. Based on intracellular reorganization of the cytoskeleton an human chorionic gonadotropin (hCG) dependent sprouting and migration of luteinizing granulosa cells (LGCs) and endothelial cells is observed. Rho-GTPases are shown to be key regulators of cytoskeletal restructuring. In the present study we analyzed the role of Rho-GTPases in the sprouting activity of LGCs.

Methods

We used the Rho-GTPase-inhibitors Toxin A and -B and the Cdc42-activator Bradykinin in a LGC-spheroid sprouting assay to determine the effect of these modulators in LGCs.

Results

Toxin A and Toxin B reduces sprout formation in LGC spheroids. However, the reduction is less than in hCG treated cells. The usage of Bradykinin demonstrates both, a reduction of sprouts in untreated spheroids and an increase of sprouting in previous hCG treated spheroids.

Conclusions

The presented results let us suggest that small Rho-GTPases may regulate the sprouting activity of LGCs after stimulation by hCG and that this mechanism may play a role in CL formation.

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Title: Small GTPases are involved in sprout formation in human granulosa lutein cells
Authors: Maximilian B. Franz
Stefanie Daube
Christoph Keck
Michael Sator
Detlef Pietrowski
Publication date: 01-04-2013
Publisher: Springer-Verlag
Published in: Archives of Gynecology and Obstetrics / Issue 4/2013
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-012-2642-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Small GTPases are involved in sprout formation in human granulosa lutein cells

Abstract

Introduction

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Introduction

Methods

Results

Conclusions

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