Top

Published in:

01-02-2020 | Skin Graft | Original Article

Effects of red and near-infrared LED light therapy on full-thickness skin graft in rats

Authors: Cintia Cristina Santi Martignago, Carla Roberta Tim, Lívia Assis, Viviane Ribeiro Da Silva, Estefany Camila Bonfim Dos Santos, Fabiana Nascimento Vieira, Nivaldo Antonio Parizotto, Richard Eloin Liebano

Published in: Lasers in Medical Science | Issue 1/2020

Abstract

The aim of the present study was to evaluate the in vivo response of different wavelengths (red and near-infrared) of light-emitting diode (LED) on full-thickness skin grafts (FTSG) in rats. Thirty rats were randomly allocated into three experimental groups: control group (C); red LED treated group (R); and near-infrared LED group (NIR). Skin grafts were irradiated daily for ten consecutive days, starting immediately after the surgery using a red (630 nm) or near-infrared (850 nm) LED. The results showed that the red wavelength LED significantly enhanced the skin graft score in relation to the NIR group and increased transforming growth factor beta (TGF-β) protein expression and density of collagen fibers compared with the other experimental groups. These results suggest that the red wavelength LED was efficient to improve the dermo-epidermal junction and modulate the expression proteins related to tissue repair.

Oganesyan G, Jarell AD, Srivastava M, Jiang B (2013) Efficacy and complication rates of full-thickness skin graft repair of lower extremity wounds after mohs micrographic surgery. Dermatologic Surg 39:1334–1339. https://doi.org/10.1111/dsu.12254 CrossRef

Audrain H, Bray A, De Berker D (2015) Full-thickness skin grafts for lower leg defects: an effective repair option. Dermatol Surg 41:493–498. https://doi.org/10.1097/DSS.0000000000000330 CrossRefPubMed

Politis MJ, Zanakis MF (1989) Enhanced survival of full-thickness skin grafts following the application of DC electrical fields. Plast Reconstr Surg 84:267–272CrossRef

Wang J, Hao H, Huang H et al (2016) The effect of adipose-derived stem cells on full-thickness skin grafts. Biomed Res Int 2016. https://doi.org/10.1155/2016/1464725

Stanley BJ, Pitt KA, Weder CD et al (2013) Effects of negative pressure wound therapy on healing of free full-thickness skin grafts in dogs. Vet Surg 42:511–522. https://doi.org/10.1111/j.1532-950X.2013.12005.x CrossRefPubMedPubMedCentral

Waris T, Rechardt L, Kyosola K (1983) Reinnervation of human skin grafts: a histocemical. Sutdy Plast Reconstr Surg 72:439–445CrossRef

Zhang F, Lineaweaver W (2011) Acute and sustained effects of vascular endothelial growth factor on survival of flaps and skin grafts. Ann Plast Surg 66:581–582. https://doi.org/10.1097/SAP.0b013e3182057376 CrossRefPubMed

Fang T, Lineaweaver WC, Chen MB et al (2014) Effects of vascular endothelial growth factor on survival of surgical flaps: a review of experimental studies. J Reconstr Microsurg 30:1–14. https://doi.org/10.1055/s-0033-1345429 CrossRefPubMed

Vaghardoost R, Momeni M, Kazemikhoo N et al (2018) Effect of low-level laser therapy on the healing process of donor site in patients with grade 3 burn ulcer after skin graft surgery ( a randomized clinical trial ). Lasers Med Sci 43:603–607. https://doi.org/10.1007/s10103-017-2430-4 CrossRef

10.

Dahmardehei M, Kazemikhoo N, Vaghardoost R et al (2016) Effects of low level laser therapy on the prognosis of split-thickness skin graft in type 3 burn of diabetic patients: a case series. Lasers Med Sci 31:497–502. https://doi.org/10.1007/s10103-016-1896-9 CrossRefPubMed

11.

Anders JJ (2015) Low-level light/laser therapy versus photobiomodulation. Therapy 1(33):183–184. https://doi.org/10.1089/pho.2015.9848 CrossRef

12.

Avci P, Gupta A, Sadasivam M et al (2013) Low-level laser (light) therapy (LLLT) in skin: stimulating, healing, restoring. Semin Cutan Med Surg 32:41–52PubMedPubMedCentral

13.

Prindeze NJ, Moffatt LT, Shupp JW (2012) Mechanisms of action for light therapy: a review of molecular interactions. Exp Biol Med 237:1241–1248. https://doi.org/10.1258/ebm.2012.012180 CrossRef

14.

Gupta A, Dai T, Hamblin MR (2014) Effect of red and near-infrared wavelengths on low-level laser ( light ) therapy-induced healing of partial-thickness dermal abrasion in mice. 257–265. https://doi.org/10.1007/s10103-013-1319-0 CrossRef

15.

Vladimirov YA, Osipov AN, Klebanov GI (2004) Photobiological principles of therapeutic applications of laser radiation. Biochem 69:81–90. https://doi.org/10.1023/B:BIRY.0000016356.93968.7e CrossRef

16.

De Freitas LF, Hamblin MR (2017) Proposed mechanisms of photobiomodulation or low-level light. Therapy:1–37. https://doi.org/10.1109/JSTQE.2016.2561201.Proposed

17.

Karu TI, Kolyakov SF (2005) Exact action spectra for cellular responses relevant to phototherapy. Photomed Laser Surg 23:355–361. https://doi.org/10.1089/pho.2005.23.355 CrossRefPubMed

18.

Em M, Chaves A Piancastelli CC effects of low-power light therapy on wound healing, pp 616–623. https://doi.org/10.1590/abd1806-4841.20142519 CrossRef

19.

Heiskanen V, Hamblin MR (2018) Photobiomodulation: lasers: vs. light emitting diodes? Photochem Photobiol Sci 17:1003–1017. https://doi.org/10.1039/c8pp00176f CrossRefPubMedPubMedCentral

20.

Richter GT, Fan CY, Ozgursoy O, Mccoy J, Vural E (2006) Effect of vascular endothelial growth factor on skin graft survival in Sprague-Dawley rats. Arch Otolaryngol Head Neck Surg 132:637–641CrossRef

21.

Martignago CCS, Tim CR, Assis L et al (2018) Comparison of two different laser photobiomodulation protocols on the viability of random skin flap in rats. Lasers Med Sci. https://doi.org/10.1007/s10103-018-2694-3 CrossRef

22.

Başaran Ö, Özdemir H, Kut A et al (2006) Effects of different preservation solutions on skin graft epidermal cell viability and graft performance in a rat model. Burns 32:423–429. https://doi.org/10.1016/j.burns.2005.11.010 CrossRefPubMed

23.

Zografou A, Tsigris C, Papadopoulos O et al (2011) Improvement of skin-graft survival after autologous transplantation of adipose-derived stem cells in rats. J Plast Reconstr Aesthet Surg 64:1647–1656. https://doi.org/10.1016/j.bjps.2011.07.009 CrossRefPubMed

24.

Nishioka MA, Pinfildi CE, Sheliga TR et al (2012) LED (660 nm) and laser (670 nm) use on skin flap viability: angiogenesis and mast cells on transition line. Lasers Med Sci 27:1045–1050. https://doi.org/10.1007/s10103-011-1042-7 CrossRefPubMed

25.

Park IS, Chung PS, Ahn JC (2015) Enhancement of ischemic wound healing by spheroid grafting of human adipose-derived stem cells treated with low-level light irradiation. PLoS One 10:1–16. https://doi.org/10.1371/journal.pone.0122776 CrossRef

26.

Fujihara Y, Koyama H, Nishiyama N et al (2005) Gene transfer of bFGF to recipient bed improves survival of ischemic skin flap. Br J Plast Surg 58:511–517. https://doi.org/10.1016/j.bjps.2004.12.028 CrossRefPubMed

27.

Fayazzadeh E, Ahmadi SH, Rabbani S et al (2012) A comparative study of recombinant human basic fibroblast growth factor (bFGF) and erythropoietin (EPO) in prevention of skin flap ischemic necrosis in rats. Arch Iran Med 15:553–556 https://doi.org/012159/AIM.008 PubMed

28.

Yu W, Naim JO, Lanzafame RJ (1994) The effect of laser irradation on the release of bFGF from 3T3 fibroblasts. Photochem Photobiol 59:167–170. https://doi.org/10.1111/j.1751-1097.1994.tb05017.x CrossRefPubMed

29.

Penn JW, Grobbelaar AO, Rolfe KJ (2012) TGF-β family in wound healing. Int J Burn Trauma 2:18–28

30.

Kasuya A, Tokura Y (2014) Attempts to accelerate wound healing. J Dermatol Sci 76:169–172. https://doi.org/10.1016/j.jdermsci.2014.11.001 CrossRefPubMed

31.

He T, Quan T, Shao Y et al (2014) Oxidative exposure impairs TGF-β pathway via reduction of type II receptor and SMAD3 in human skin fibroblasts. Age (Omaha) 36:1079–1094. https://doi.org/10.1007/s11357-014-9623-6 CrossRef

32.

Nogueira VC, Coelho NPM de F, de BTL et al (2014) Biomodulation effects of LED and therapeutic ultrasound combined with semipermeable dressing in the repair process of cutaneous lesions in rats. Acta Cir Bras 29:588–595. https://doi.org/10.1590/S0102-8650201400150006 CrossRefPubMed

33.

Safavi SM, Kazemi B, Esmaeili M et al (2008) Effects of low-level He-Ne laser irradiation on the gene expression of IL-1β, TNF-α, IFN-γ, TGF-β, bFGF, and PDGF in rat’s gingiva. Lasers Med Sci 23:331–335. https://doi.org/10.1007/s10103-007-0491-5 CrossRefPubMed

34.

de Sousa APC, Santos JN, dos Reis JA et al (2010) Effect of LED phototherapy of three distinct wavelengths on fibroblasts on wound healing: a histological study in a rodent model. Photomed Laser Surg 28:547–552. https://doi.org/10.1089/pho.2009.2605 CrossRefPubMed

Title: Effects of red and near-infrared LED light therapy on full-thickness skin graft in rats
Authors: Cintia Cristina Santi Martignago
Carla Roberta Tim
Lívia Assis
Viviane Ribeiro Da Silva
Estefany Camila Bonfim Dos Santos
Fabiana Nascimento Vieira
Nivaldo Antonio Parizotto
Richard Eloin Liebano
Publication date: 01-02-2020
Publisher: Springer London
Keyword: Skin Graft
Published in: Lasers in Medical Science / Issue 1/2020
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-019-02812-6

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Effects of red and near-infrared LED light therapy on full-thickness skin graft in rats

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 1/2020

The impact of photobiomodulation of major salivary glands on caries risk

Photobiomodulation therapy at different wavelength impacts on retinoid acid–dependent SH-SY5Y differentiation

Bioluminescence and near-infrared fluorescence imaging for detection of metastatic bone tumors

Laser-assisted endoscopic lumbar foraminotomy for failed back surgery syndrome in elderly patients

Low-fluence Q-switched Nd:YAG 1064-nm laser versus Q-switched Nd:YAG 532-nm laser in the treatment of hyperpigmented lips: a prospective, randomized, controlled, evaluator-blinded trial

Utilizing 808 nm laser for sensitizing of melanoma tumors to megavoltage radiation therapy