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Forensic Toxicology
Published in: Forensic Toxicology 2/2016

01-07-2016 | Original Article

Simultaneous determination of cyclic antidepressants and their related drugs and the estimation of new metabolites in human whole blood and urine by MALDI-QTOF-mass spectrometry

Authors: Hideki Nozawa, Kayoko Minakata, Itaru Yamagishi, Koutaro Hasegawa, Masako Suzuki, Kunio Gonmori, Osamu Suzuki, Kanako Watanabe

Published in: Forensic Toxicology | Issue 2/2016

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Abstract

A rapid and sensitive method using matrix-assisted laser desorption ionization (MALDI)-quadrupole time-of-flight (QTOF)-mass spectrometry (MS) was developed for the analysis of six tricyclic antidepressants (ADs) and their related drugs, such as amitriptyline, carbamazepine, clomipramine, imipramine, nortriptyline, quetiapine, and two tetracyclic ADs, mianserin and mirtazapine, because these eight drugs are commonly observed medicines in poisoning cases in Japan. In the present MALDI-MS method, the limits of detection were 0.3–2 ng/mL. The present method provided a simple and high throughput simultaneous analysis of these drugs. This is the first report dealing with quantitation of ADs and their related drugs in biological samples by MALDI-MS. In addition, we applied the MALDI-MS to two authentic cases, and three new metabolites of amitriptyline and three new metabolites of quetiapine could be detected in blood and urine samples in the two cases when regioisomers were counted as one metabolite.
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Metadata
Title
Simultaneous determination of cyclic antidepressants and their related drugs and the estimation of new metabolites in human whole blood and urine by MALDI-QTOF-mass spectrometry
Authors
Hideki Nozawa
Kayoko Minakata
Itaru Yamagishi
Koutaro Hasegawa
Masako Suzuki
Kunio Gonmori
Osamu Suzuki
Kanako Watanabe
Publication date
01-07-2016
Publisher
Springer Japan
Published in
Forensic Toxicology / Issue 2/2016
Print ISSN: 1860-8965
Electronic ISSN: 1860-8973
DOI
https://doi.org/10.1007/s11419-016-0313-1

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