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Breast Cancer Research and Treatment
Published in: Breast Cancer Research and Treatment 1/2012

01-01-2012 | Brief Report

Simultaneous analysis of tumor and stromal gene expression profiles from xenograft models

Authors: Elizabeth Iorns, Jennifer Clarke, Toby Ward, Sonja Dean, Marc Lippman

Published in: Breast Cancer Research and Treatment | Issue 1/2012

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Abstract

Identifying the gene expression alterations that occur in both the tumor and stroma is essential to understanding tumor biology. We have developed a dual-species microarray analysis method that allows the dissection of both tumor and stromal gene expression profiles from xenograft models, based on limited interspecies cross-hybridization on Illumina gene expression beadchips. This methodology allows for simultaneous genome-wide analysis of gene expression profiles of both tumor cells and the associated stromal tissue.
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Literature
1.
Fidler IJ, Kim SJ, Langley RR (2007) The role of the organ microenvironment in the biology and therapy of cancer metastasis. J Cell Biochem 101:927–936PubMedCrossRef
2.
Perou CM, Sørlie T, Eisen MB, van de Rijn M, Jeffrey SS, Rees CA et al (2000) Molecular portraits of human breast tumours. Nature 406:747–752PubMedCrossRef
3.
Finak G, Bertos N, Pepin F, Sadekova S, Souleimanova M, Zhao H et al (2008) Stromal gene expression predicts clinical outcome in breast cancer. Nat Med 14:518–527PubMedCrossRef
4.
Francia G, Cruz-Munoz W, Man S, Xu P, Kerbel RS (2011) Mouse models of advanced spontaneous metastasis for experimental therapeutics. Nat Rev Cancer 11:135–141PubMedCrossRef
5.
Serra R, Easter SL, Jiang W, Baxley SE (2011) Wnt5a as an effector of TGFβ in mammary development and cancer. J Mammary Gland Biol Neoplasia 16:157–167PubMedCrossRef
6.
Bourboulia D, Stetler-Stevenson WG (2010) Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs): positive and negative regulators in tumor cell adhesion. Semin Cancer Biol 20:161–168PubMedCrossRef
7.
Du P, Kibbe WA, Lin SM (2008) Lumi: a pipeline for processing illumina microarray. Bioinformatics 24:1547–1548PubMedCrossRef
8.
R Development Core Team (2011) Vienna, Austria
Metadata
Title
Simultaneous analysis of tumor and stromal gene expression profiles from xenograft models
Authors
Elizabeth Iorns
Jennifer Clarke
Toby Ward
Sonja Dean
Marc Lippman
Publication date
01-01-2012
Publisher
Springer US
Published in
Breast Cancer Research and Treatment / Issue 1/2012
Print ISSN: 0167-6806
Electronic ISSN: 1573-7217
DOI
https://doi.org/10.1007/s10549-011-1784-8

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