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Annals of Surgical Oncology
Published in: Annals of Surgical Oncology 13/2021

Open Access 01-12-2021 | Translational Research

Significance of Circular FAT1 as a Prognostic Factor and Tumor Suppressor for Esophageal Squamous Cell Carcinoma

Authors: Wataru Takaki, MD, Hirotaka Konishi, MD, PhD, Katsutoshi Shoda, MD, PhD, Tomohiro Arita, MD, PhD, Satoshi Kataoka, MD, Jun Shibamoto, MD, Hirotaka Furuke, MD, Kazuya Takabatake, MD, Hiroki Shimizu, MD, PhD, Shuhei Komatsu, MD, PhD, Atsushi Shiozaki, MD, PhD, Hitoshi Fujiwara, MD, PhD, Kiyoshi Masuda, MD, PhD, Eigo Otsuji, MD, PhD

Published in: Annals of Surgical Oncology | Issue 13/2021

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Abstract

Background

Circular RNA is a novel endogenous non-coding RNA with a stable loop structure, and theories for its biogenesis and usefulness as a biomarker in various cancers have been proposed. The present study investigated the significance of circular FAT1 (circFAT1) as a novel biomarker in esophageal squamous cell carcinoma (ESCC).

Method

CircFAT1 expression levels were measured in ESCC cell lines and the effects of downregulating circFAT1 on cell migration and invasion were examined using a transwell assay. The functions of miR-548g, which will be sponged by circFAT1, were assessed. Furthermore, the expression of circFAT1 was evaluated in 51 radically resected ESCC tissue samples using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The relationships between circFAT1 expression, clinicopathological factors, and patient prognosis were analyzed.

Results

CircFAT1 expression levels were significantly lower in tumor tissue than in adjacent non-tumorous mucosal tissue (p = 0.01). The downregulation of circFAT1 expression promoted ESCC cell migration and invasive ability, but not proliferation. The expression of miR-548g was upregulated by the downregulation of circFAT1. The overexpression of miR-548g also promoted ESCC cell migration and invasion. Recurrence-free survival (p = 0.02) and cancer-specific survival (p = 0.04) rates were significantly higher in patients with elevated circFAT1 expression levels.

Conclusion

The expression level of circFAT1 is a novel prognostic marker in ESCC patients. New treatment strategies may be developed using the tumor suppressive functions of circFAT1.
Appendix
Available only for authorised users
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Metadata
Title
Significance of Circular FAT1 as a Prognostic Factor and Tumor Suppressor for Esophageal Squamous Cell Carcinoma
Authors
Wataru Takaki, MD
Hirotaka Konishi, MD, PhD
Katsutoshi Shoda, MD, PhD
Tomohiro Arita, MD, PhD
Satoshi Kataoka, MD
Jun Shibamoto, MD
Hirotaka Furuke, MD
Kazuya Takabatake, MD
Hiroki Shimizu, MD, PhD
Shuhei Komatsu, MD, PhD
Atsushi Shiozaki, MD, PhD
Hitoshi Fujiwara, MD, PhD
Kiyoshi Masuda, MD, PhD
Eigo Otsuji, MD, PhD
Publication date
01-12-2021
Publisher
Springer International Publishing
Published in
Annals of Surgical Oncology / Issue 13/2021
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI
https://doi.org/10.1245/s10434-021-10089-9

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