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01-08-2021 | Shock | Original Article

Photobiomodulation therapy on expression of HSP70 protein and tissue repair in experimental acute Achilles tendinitis

Authors: André Nobre Evangelista, Flávia Franciele dos Santos, Lorena Pacheco de Oliveira Martins, Thais Peixoto Gaiad, Alex Sander Dias Machado, Etel Rocha-Vieira, Karine Beatriz Costa, Ana Paula Santos, Murilo Xavier Oliveira

Published in: Lasers in Medical Science | Issue 6/2021

Abstract

The aim of the present study was to investigate the effects of photobiomodulation (PBM) therapy on the expression of heat shock protein 70 (HSP70) and tissue repair in an experimental model of collagenase-induced Achilles tendinitis. Thirty Wistar rats (aged 12 weeks) were randomly distributed among control group (n = 8), tendinitis group (n = 11), and LED group (n = 11). Tendinitis was induced in the tendinitis and LED groups through a peritendinous injection of collagenase (100 μl). The LED group animals received the first irradiation 1 h after injury. A 630 ± 20 nm, 300-mW continuous wave light-emitting diode (LED), spot size 1 cm², was placed in contact with the skin. One point over the tendon was irradiated for 30 s, delivering 9 J (9 J/cm²). LED irradiation was performed once daily for 7 days, with the total energy delivered being 63 J. The tendons were surgically removed and expression of the HSP70 protein was calculated using semi-quantitative analyses of immunohistochemistry (HSCORE). Number of fibroblasts and amount of collagen were measured using histological and histochemical analyses. An increase in the mean HSCORE for HSP70, in the number of fibroblasts, and in the amount of collagen were found in the LED group compared with those in the tendinitis and control group (P ≤ 0.05). PBM therapy increased the expression of the HSP70, number of fibroblasts, and amount of collagen in the acute Achilles tendinitis in rats.

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Title: Photobiomodulation therapy on expression of HSP70 protein and tissue repair in experimental acute Achilles tendinitis
Authors: André Nobre Evangelista
Flávia Franciele dos Santos
Lorena Pacheco de Oliveira Martins
Thais Peixoto Gaiad
Alex Sander Dias Machado
Etel Rocha-Vieira
Karine Beatriz Costa
Ana Paula Santos
Murilo Xavier Oliveira
Publication date: 01-08-2021
Publisher: Springer London
Keywords: Shock
Shock
Tendinopathy
Tendinitis
Published in: Lasers in Medical Science / Issue 6/2021
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-020-03155-3

At a glance: The STEP trials

Springer Medicine

Photobiomodulation therapy on expression of HSP70 protein and tissue repair in experimental acute Achilles tendinitis

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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