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Virology Journal
Published in: Virology Journal 1/2011

Open Access 01-12-2011 | Research

Sequencing of bovine herpesvirus 4 v.test strain reveals important genome features

Authors: Leonor Palmeira, Bénédicte Machiels, Céline Lété, Alain Vanderplasschen, Laurent Gillet

Published in: Virology Journal | Issue 1/2011

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Abstract

Background

Bovine herpesvirus 4 (BoHV-4) is a useful model for the human pathogenic gammaherpesviruses Epstein-Barr virus and Kaposi's Sarcoma-associated Herpesvirus. Although genome manipulations of this virus have been greatly facilitated by the cloning of the BoHV-4 V.test strain as a Bacterial Artificial Chromosome (BAC), the lack of a complete genome sequence for this strain limits its experimental use.

Methods

In this study, we have determined the complete sequence of BoHV-4 V.test strain by a pyrosequencing approach.

Results

The long unique coding region (LUR) consists of 108,241 bp encoding at least 79 open reading frames and is flanked by several polyrepetitive DNA units (prDNA). As previously suggested, we showed that the prDNA unit located at the left prDNA-LUR junction (prDNA-G) differs from the other prDNA units (prDNA-inner). Namely, the prDNA-G unit lacks the conserved pac-2 cleavage and packaging signal in its right terminal region. Based on the mechanisms of cleavage and packaging of herpesvirus genomes, this feature implies that only genomes bearing left and right end prDNA units are encapsulated into virions.

Conclusions

In this study, we have determined the complete genome sequence of the BAC-cloned BoHV-4 V.test strain and identified genome organization features that could be important in other herpesviruses.
Appendix
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Metadata
Title
Sequencing of bovine herpesvirus 4 v.test strain reveals important genome features
Authors
Leonor Palmeira
Bénédicte Machiels
Céline Lété
Alain Vanderplasschen
Laurent Gillet
Publication date
01-12-2011
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2011
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-8-406

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