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European Journal of Clinical Microbiology & Infectious Diseases

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Open Access 01-03-2022 | Septicemia | Brief Report

Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures

Authors: Katharina Bach, Birgit Edel, Steffen Höring, Lucie Bartoničkova, Stefan Glöckner, Bettina Löffler, Christina Bahrs, Jürgen Rödel

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 3/2022

Abstract

The LAMP-based eazyplex® BloodScreen GN was evaluated for the detection of frequent Gram-negatives directly from positive blood culture (BC) bottles. A total of 449 BCs were analyzed. Sensitivities and specificities were 100% and 100% for Escherichia coli, 95.7% and 100% for Klebsiella pneumoniae, 100% and 100% for bla_CTX-M, 100% and 100% for Klebsiella oxytoca, 100% and 99% for Proteus mirabilis, and 100% and 99.8% for Pseudomonas aeruginosa, respectively. The time to result ranged from 8 to 16 min, plus about 6 min for sample preparation. The eazyplex® BloodScreen GN is a reliable molecular assay for rapid BC testing.

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Title: Performance of the eazyplex® BloodScreen GN as a simple and rapid molecular test for identification of Gram-negative bacteria from positive blood cultures
Authors: Katharina Bach
Birgit Edel
Steffen Höring
Lucie Bartoničkova
Stefan Glöckner
Bettina Löffler
Christina Bahrs
Jürgen Rödel
Publication date: 01-03-2022
Publisher: Springer Berlin Heidelberg
Keywords: Septicemia
Septicemia
Piperacillin
Meropenem
Pseudomonas Aeruginosa
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 3/2022
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-021-04383-3

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