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Malaria Journal
Published in: Malaria Journal 1/2018

Open Access 01-12-2018 | Research

Self-assembling functional programmable protein array for studying protein–protein interactions in malaria parasites

Authors: Gabriela Arévalo-Pinzón, María González-González, Carlos Fernando Suárez, Hernando Curtidor, Javier Carabias-Sánchez, Antonio Muro, Joshua LaBaer, Manuel Alfonso Patarroyo, Manuel Fuentes

Published in: Malaria Journal | Issue 1/2018

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Abstract

Background

Plasmodium vivax is the most widespread malarial species, causing significant morbidity worldwide. Knowledge is limited regarding the molecular mechanism of invasion due to the lack of a continuous in vitro culture system for these species. Since protein–protein and host–cell interactions play an essential role in the microorganism’s invasion and replication, elucidating protein function during invasion is critical when developing more effective control methods. Nucleic acid programmable protein array (NAPPA) has thus become a suitable technology for studying protein–protein and host–protein interactions since producing proteins through the in vitro transcription/translation (IVTT) method overcomes most of the drawbacks encountered to date, such as heterologous protein production, stability and purification.

Results

Twenty P. vivax proteins on merozoite surface or in secretory organelles were selected and successfully cloned using gateway technology. Most constructs were displayed in the array expressed in situ, using the IVTT method. The Pv12 protein was used as bait for evaluating array functionality and co-expressed with P. vivax cDNA display in the array. It was found that Pv12 interacted with Pv41 (as previously described), as well as PvMSP142kDa, PvRBP1a, PvMSP8 and PvRAP1.

Conclusions

NAPPA is a high-performance technique enabling co-expression of bait and query in situ, thereby enabling interactions to be analysed rapidly and reproducibly. It offers a fresh alternative for studying protein–protein and ligand–receptor interactions regarding a parasite which is difficult to cultivate (i.e. P. vivax).
Appendix
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Literature
1.
Mueller I, Galinski MR, Baird JK, Carlton JM, Kochar DK, Alonso PL, et al. Key gaps in the knowledge of Plasmodium vivax, a neglected human malaria parasite. Lancet Infect Dis. 2009;9:555–66.CrossRefPubMed
2.
Noulin F, Borlon C, Van Den Abbeele J, D’Alessandro U, Erhart A. 1912-2012: a century of research on Plasmodium vivax in vitro culture. Trends Parasitol. 2013;29:286–94.CrossRefPubMed
3.
Weiss GE, Gilson PR, Taechalertpaisarn T, Tham WH, de Jong NW, Harvey KL, et al. Revealing the sequence and resulting cellular morphology of receptor–ligand interactions during Plasmodium falciparum invasion of erythrocytes. PLoS Pathog. 2015;11:e1004670.CrossRefPubMedPubMedCentral
4.
Lopaticki S, Maier AG, Thompson J, Wilson DW, Tham WH, Triglia T, et al. Reticulocyte and erythrocyte binding-like proteins function cooperatively in invasion of human erythrocytes by malaria parasites. Infect Immun. 2011;79:1107–17.CrossRefPubMed
5.
Stubbs J, Simpson KM, Triglia T, Plouffe D, Tonkin CJ, Duraisingh MT, et al. Molecular mechanism for switching of P. falciparum invasion pathways into human erythrocytes. Science. 2005;309:1384–7.CrossRefPubMed
6.
Lin CS, Uboldi AD, Marapana D, Czabotar PE, Epp C, Bujard H, et al. The merozoite surface protein 1 complex is a platform for binding to human erythrocytes by Plasmodium falciparum. J Biol Chem. 2014;289:25655–69.CrossRefPubMedPubMedCentral
7.
Kauth CW, Woehlbier U, Kern M, Mekonnen Z, Lutz R, Mucke N, et al. Interactions between merozoite surface proteins 1, 6, and 7 of the malaria parasite Plasmodium falciparum. J Biol Chem. 2006;281:31517–27.CrossRefPubMed
8.
Ranjan R, Chugh M, Kumar S, Singh S, Kanodia S, Hossain MJ, et al. Proteome analysis reveals a large merozoite surface protein-1 associated complex on the Plasmodium falciparum merozoite surface. J Proteome Res. 2011;10:680–91.CrossRefPubMed
9.
Wanaguru M, Crosnier C, Johnson S, Rayner JC, Wright GJ. Biochemical analysis of the Plasmodium falciparum erythrocyte-binding antigen-175 (EBA175)-glycophorin-A interaction: implications for vaccine design. J Biol Chem. 2013;288:32106–17.CrossRefPubMedPubMedCentral
10.
Batchelor JD, Malpede BM, Omattage NS, DeKoster GT, Henzler-Wildman KA, Tolia NH. Red blood cell invasion by Plasmodium vivax: structural basis for DBP engagement of DARC. PLoS Pathog. 2014;10:e1003869.CrossRefPubMedPubMedCentral
11.
Li X, Chen H, Oo TH, Daly TM, Bergman LW, Liu SC, Chishti AH, et al. A co-ligand complex anchors Plasmodium falciparum merozoites to the erythrocyte invasion receptor band 3. J Biol Chem. 2004;279:5765–71.CrossRefPubMed
12.
Lin CS, Uboldi AD, Epp C, Bujard H, Tsuboi T, Czabotar PE, et al. Multiple Plasmodium falciparum merozoite surface protein 1 complexes mediate merozoite binding to human erythrocytes. J Biol Chem. 2016;291:7703–15.CrossRefPubMedPubMedCentral
13.
Baldwin MR, Li X, Hanada T, Liu SC, Chishti AH. Merozoite surface protein 1 recognition of host glycophorin A mediates malaria parasite invasion of red blood cells. Blood. 2015;125:2704–11.CrossRefPubMedPubMedCentral
14.
15.
Patarroyo MA, Calderon D, Moreno-Perez DA. Vaccines against Plasmodium vivax: a research challenge. Expert Rev Vaccines. 2012;11:1249–60.CrossRefPubMed
16.
17.
Mehlin C, Boni E, Buckner FS, Engel L, Feist T, Gelb MH, et al. Heterologous expression of proteins from Plasmodium falciparum: results from 1000 genes. Mol Biochem Parasitol. 1000;2006(148):144–60.
18.
Gardner MJ, Hall N, Fung E, White O, Berriman M, Hyman RW, et al. Genome sequence of the human malaria parasite Plasmodium falciparum. Nature. 2002;419:498–511.CrossRefPubMed
19.
Hillebrecht JR, Chong S. A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based. BMC Biotechnol. 2008;8:58.CrossRefPubMedPubMedCentral
20.
Hino M, Kataoka M, Kajimoto K, Yamamoto T, Kido J, Shinohara Y, et al. Efficiency of cell-free protein synthesis based on a crude cell extract from Escherichia coli, wheat germ, and rabbit reticulocytes. J Biotechnol. 2008;133:183–9.CrossRefPubMed
21.
Diez P, Dasilva N, Gonzalez-Gonzalez M, Matarraz S, Casado-Vela J, Orfao A, et al. Data analysis strategies for protein microarrays. Microarrays (Basel). 2012;1:64–83.CrossRef
22.
Manzano-Roman R, Dasilva N, Diez P, Diaz-Martin V, Perez-Sanchez R, Orfao A, et al. Protein arrays as tool for studies at the host–pathogen interface. J Proteomics. 2013;94:387–400.CrossRefPubMed
23.
Tsuboi T, Takeo S, Iriko H, Jin L, Tsuchimochi M, Matsuda S, et al. Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates. Infect Immun. 2008;76:1702–8.CrossRefPubMedPubMedCentral
24.
Doolan DL, Mu Y, Unal B, Sundaresh S, Hirst S, Valdez C, et al. Profiling humoral immune responses to P. falciparum infection with protein microarrays. Proteomics. 2008;8:4680–94.CrossRefPubMedPubMedCentral
25.
Mu J, Awadalla P, Duan J, McGee KM, Keebler J, Seydel K, et al. Genome-wide variation and identification of vaccine targets in the Plasmodium falciparum genome. Nat Genet. 2007;39:126–30.CrossRefPubMed
26.
Yadavalli R, Sam-Yellowe T. HeLa Based cell free expression systems for expression of Plasmodium rhoptry proteins. J Vis Exp. 2015:e52772.
27.
Zemella A, Thoring L, Hoffmeister C, Kubick S. Cell-free protein synthesis: pros and cons of prokaryotic and eukaryotic systems. ChemBioChem. 2015;16:2420–31.CrossRefPubMedPubMedCentral
28.
Chen JH, Jung JW, Wang Y, Ha KS, Lu F, Lim CS, et al. Immunoproteomics profiling of blood stage Plasmodium vivax infection by high-throughput screening assays. J Proteome Res. 2010;9:6479–89.CrossRefPubMed
29.
Ramachandran N, Raphael JV, Hainsworth E, Demirkan G, Fuentes MG, Rolfs A, et al. Next-generation high-density self-assembling functional protein arrays. Nat Methods. 2008;5:535–8.CrossRefPubMedPubMedCentral
30.
Rui E, Fernandez-Becerra C, Takeo S, Sanz S, Lacerda MV, Tsuboi T, et al. Plasmodium vivax: comparison of immunogenicity among proteins expressed in the cell-free systems of Escherichia coli and wheat germ by suspension array assays. Malar J. 2011;10:192.CrossRefPubMedPubMedCentral
31.
Dent AE, Nakajima R, Liang L, Baum E, Moormann AM, Sumba PO, et al. Plasmodium falciparum protein microarray antibody profiles correlate with protection from symptomatic malaria in Kenya. J Infect Dis. 2015;212:1429–38.CrossRefPubMedPubMedCentral
32.
Molina DM, Finney OC, Arevalo-Herrera M, Herrera S, Felgner PL, Gardner MJ, et al. Plasmodium vivax pre-erythrocytic-stage antigen discovery: exploiting naturally acquired humoral responses. Am J Trop Med Hyg. 2012;87:460–9.CrossRefPubMedPubMedCentral
33.
Carlson ED, Gan R, Hodgman CE, Jewett MC. Cell-free protein synthesis: applications come of age. Biotechnol Adv. 2012;30:1185–94.CrossRefPubMed
34.
Montor WR, Huang J, Hu Y, Hainsworth E, Lynch S, Kronish JW, et al. Genome-wide study of Pseudomonas aeruginosa outer membrane protein immunogenicity using self-assembling protein microarrays. Infect Immun. 2009;7:4877–86.CrossRef
35.
Henjes F, Lourido L, Ruiz-Romero C, Fernandez-Tajes J, Schwenk JM, Gonzalez-Gonzalez M, et al. Analysis of autoantibody profiles in osteoarthritis using comprehensive protein array concepts. J Proteome Res. 2014;13:5218–29.CrossRefPubMed
36.
Yu X, Decker KB, Barker K, Neunuebel MR, Saul J, Graves M, et al. Host-pathogen interaction profiling using self-assembling human protein arrays. J Proteome Res. 2015;14:1920–36.CrossRefPubMedPubMedCentral
37.
Manzano-Roman R, Diaz-Martin V, Gonzalez-Gonzalez M, Matarraz S, Alvarez-Prado AF, LaBaer J, et al. Self-assembled protein arrays from an Ornithodoros moubata salivary gland expression library. J Proteome Res. 2012;11:5972–82.CrossRefPubMed
38.
Yap A, Azevedo MF, Gilson PR, Weiss GE, O’Neill MT, Wilson DW, et al. Conditional expression of apical membrane antigen 1 in Plasmodium falciparum shows it is required for erythrocyte invasion by merozoites. Cell Microbiol. 2014;16:642–56.CrossRefPubMedPubMedCentral
39.
Williams AR, Douglas AD, Miura K, Illingworth JJ, Choudhary P, Murungi LM, et al. Enhancing blockade of Plasmodium falciparum erythrocyte invasion: assessing combinations of antibodies against PfRH5 and other merozoite antigens. PLoS Pathog. 2012;8:e1002991.CrossRefPubMedPubMedCentral
40.
41.
42.
Gonzalez-Gonzalez M, Bartolome R, Jara-Acevedo R, Casado-Vela J, Dasilva N, Matarraz S, et al. Evaluation of homo- and hetero-functionally activated glass surfaces for optimized antibody arrays. Anal Biochem. 2014;50:37–45.CrossRef
43.
Katzen F. Gateway((R)) recombinational cloning: a biological operating system. Expert Opin Drug Discov. 2007;2:571–89.CrossRefPubMed
44.
Hunt I. From gene to protein: a review of new and enabling technologies for multi-parallel protein expression. Protein Expr Purif. 2005;40:1–22.CrossRefPubMed
45.
Hostetler JB, Sharma S, Bartholdson SJ, Wright GJ, Fairhurst RM, Rayner JC. A library of Plasmodium vivax recombinant merozoite proteins reveals new vaccine candidates and protein–protein interactions. PLoS Negl Trop Dis. 2015;9:e0004264.CrossRefPubMedPubMedCentral
46.
Moreno-Perez DA, Areiza-Rojas R, Florez-Buitrago X, Silva Y, Patarroyo ME, Patarroyo MA. The GPI-anchored 6-Cys protein Pv12 is present in detergent-resistant microdomains of Plasmodium vivax blood stage schizonts. Protist. 2013;164:37–48.CrossRefPubMed
47.
Sanders PR, Cantin GT, Greenbaum DC, Gilson PR, Nebl T, Moritz RL, et al. Identification of protein complexes in detergent-resistant membranes of Plasmodium falciparum schizonts. Mol Biochem Parasitol. 2007;154:148–57.CrossRefPubMed
48.
49.
Wells JA, McClendon CL. Reaching for high-hanging fruit in drug discovery at protein–protein interfaces. Nature. 2007;450:1001–9.CrossRefPubMed
50.
Srinivasan P, Yasgar A, Luci DK, Beatty WL, Hu X, Andersen J, et al. Disrupting malaria parasite AMA1–RON2 interaction with a small molecule prevents erythrocyte invasion. Nat Commun. 2013;4:2261.CrossRefPubMedPubMedCentral
51.
Moreno-Perez DA, Degano R, Ibarrola N, Muro A, Patarroyo MA. Determining the Plasmodium vivax VCG-1 strain blood stage proteome. J Proteomics. 2014;113C:268–80.PubMed
52.
Lu F, Li J, Wang B, Cheng Y, Kong DH, Cui L, et al. Profiling the humoral immune responses to Plasmodium vivax infection and identification of candidate immunogenic rhoptry-associated membrane antigen (RAMA). J Proteomics. 2014;102:66–82.CrossRefPubMed
53.
Acharya P, Pallavi R, Chandran S, Dandavate V, Sayeed SK, Rochani A, et al. Clinical proteomics of the neglected human malarial parasite Plasmodium vivax. PLoS ONE. 2011;6:e26623.CrossRefPubMedPubMedCentral
54.
Acharya P, Pallavi R, Chandran S, Chakravarti H, Middha S, Acharya J, et al. A glimpse into the clinical proteome of human malaria parasites Plasmodium falciparum and Plasmodium vivax. Proteomics Clin Appl. 2009;3:1314–25.CrossRefPubMed
55.
Roobsoong W, Roytrakul S, Sattabongkot J, Li J, Udomsangpetch R, Cui L. Determination of the Plasmodium vivax schizont stage proteome. J Proteomics. 2011;74:1701–10.CrossRefPubMedPubMedCentral
56.
Beeson JG, Drew DR, Boyle MJ, Feng G, Fowkes FJ, Richards JS. Merozoite surface proteins in red blood cell invasion, immunity and vaccines against malaria. FEMS Microbiol Rev. 2016;40:343–72.CrossRefPubMedPubMedCentral
57.
Perez-Leal O, Sierra AY, Barrero CA, Moncada C, Martinez P, Cortes J, et al. Identifying and characterising the Plasmodium falciparum merozoite surface protein 10 Plasmodium vivax homologue. Biochem Biophys Res Commun. 2005;331:1178–84.CrossRefPubMed
58.
Perez-Leal O, Sierra AY, Barrero CA, Moncada C, Martinez P, Cortes J, et al. Plasmodium vivax merozoite surface protein 8 cloning, expression, and characterisation. Biochem Biophys Res Commun. 2004;324:1393–9.CrossRefPubMed
59.
Black CG, Barnwell JW, Huber CS, Galinski MR, Coppel RL. The Plasmodium vivax homologues of merozoite surface proteins 4 and 5 from Plasmodium falciparum are expressed at different locations in the merozoite. Mol Biochem Parasitol. 2002;120:215–24.CrossRefPubMed
60.
Lopez C, Yepes-Perez Y, Hincapie-Escobar N, Diaz-Arevalo D, Patarroyo MA. What is known about the immune response induced by Plasmodium vivax malaria vaccine candidates? Front Immunol. 2017;8:126.PubMedPubMedCentral
61.
Singh S, Miura K, Zhou H, Muratova O, Keegan B, Miles A, et al. Immunity to recombinant Plasmodium falciparum merozoite surface protein 1 (MSP1): protection in Aotus nancymai monkeys strongly correlates with anti-MSP1 antibody titer and in vitro parasite-inhibitory activity. Infect Immun. 2006;74:4573–80.CrossRefPubMedPubMedCentral
62.
Arredondo SA, Cai M, Takayama Y, MacDonald NJ, Anderson DE, Aravind L, et al. Structure of the Plasmodium 6-cysteine s48/45 domain. Proc Natl Acad Sci USA. 2012;109:6692–7.CrossRefPubMed
63.
Taechalertpaisarn T, Crosnier C, Bartholdson SJ, Hodder AN, Thompson J, Bustamante LY, et al. Biochemical and functional analysis of two Plasmodium falciparum blood-stage 6-cys proteins: P12 and P41. PLoS ONE. 2012;7:e41937.CrossRefPubMedPubMedCentral
64.
Han JH, Lee SK, Wang B, Muh F, Nyunt MH, Na S, et al. Identification of a reticulocyte-specific binding domain of Plasmodium vivax reticulocyte-binding protein 1 that is homologous to the PfRh4 erythrocyte-binding domain. Sci Rep. 2016;6:26993.CrossRefPubMedPubMedCentral
65.
66.
Vulliez-Le Normand B, Tonkin ML, Lamarque MH, Langer S, Hoos S, Roques M, et al. Structural and functional insights into the malaria parasite moving junction complex. PLoS Pathog. 2012;8:e1002755.CrossRefPubMedPubMedCentral
67.
Zuccala ES, Gout AM, Dekiwadia C, Marapana DS, Angrisano F, Turnbull L, et al. Subcompartmentalisation of proteins in the rhoptries correlates with ordered events of erythrocyte invasion by the blood stage malaria parasite. PLoS ONE. 2012;7:e46160.CrossRefPubMedPubMedCentral
68.
Ridley RG, Takacs B, Etlinger H, Scaife JG. A rhoptry antigen of Plasmodium falciparum is protective in Saimiri monkeys. Parasitology. 1990;101(Pt 2):187–92.CrossRefPubMed
69.
70.
Moreno-Perez DA, Montenegro M, Patarroyo ME, Patarroyo MA. Identification, characterization and antigenicity of the Plasmodium vivax rhoptry neck protein 1 (PvRON1). Malar J. 2011;10:314.CrossRefPubMedPubMedCentral
71.
Mongui A, Angel DI, Moreno-Perez DA, Villarreal-Gonzalez S, Almonacid H, Vanegas M, et al. Identification and characterization of the Plasmodium vivax thrombospondin-related apical merozoite protein. Malar J. 2010;9:283.CrossRefPubMedPubMedCentral
72.
Gibbs PE, Zouzias DC, Freedberg IM. Differential post-translational modification of human type I keratins synthesized in a rabbit reticulocyte cell-free system. Biochim Biophys Acta. 1985;824:247–55.CrossRefPubMed
73.
Safer B, Jagus R. Control of eIF-2 phosphatase activity in rabbit reticulocyte lysate. Proc Natl Acad Sci USA. 1979;76:1094–8.CrossRefPubMed
74.
Shields D, Blobel G. Efficient cleavage and segregation of nascent presecretory proteins in a reticulocyte lysate supplemented with microsomal membranes. J Biol Chem. 1978;253:3753–6.PubMed
75.
Fakruddin JM, Biswas S, Sharma YD. Metalloprotease activity in a small heat shock protein of the human malaria parasite Plasmodium vivax. Infect Immun. 2000;68:1202–6.CrossRefPubMedPubMedCentral
76.
Yu X, LaBaer J. High-throughput identification of proteins with AMPylation using self-assembled human protein (NAPPA) microarrays. Nat Protoc. 2015;10:756–67.CrossRefPubMedPubMedCentral
77.
Bartholdson SJ, Crosnier C, Bustamante LY, Rayner JC, Wright GJ. Identifying novel Plasmodium falciparum erythrocyte invasion receptors using systematic extracellular protein interaction screens. Cell Microbiol. 2013;15:1304–12.CrossRefPubMedPubMedCentral
78.
Crosnier C, Bustamante LY, Bartholdson SJ, Bei AK, Theron M, Uchikawa M, et al. Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum. Nature. 2011;480:534–7.CrossRefPubMedPubMedCentral
79.
Galaway F, Drought LG, Fala M, Cross N, Kemp AC, Rayner JC, et al. P113 is a merozoite surface protein that binds the N terminus of Plasmodium falciparum RH5. Nat Commun. 2017;8:14333.CrossRefPubMedPubMedCentral
80.
LaCount DJ, Vignali M, Chettier R, Phansalkar A, Bell R, Hesselberth JR, et al. A protein interaction network of the malaria parasite Plasmodium falciparum. Nature. 2005;438:103–7.CrossRefPubMed
81.
Templin MF, Stoll D, Schrenk M, Traub PC, Vohringer CF, Joos TO. Protein microarray technology. Trends Biotechnol. 2002;20:160–6.CrossRefPubMed
82.
Bozdech Z, Mok S, Hu G, Imwong M, Jaidee A, Russell B, et al. The transcriptome of Plasmodium vivax reveals divergence and diversity of transcriptional regulation in malaria parasites. Proc Natl Acad Sci USA. 2008;105:16290–5.CrossRefPubMed
Metadata
Title
Self-assembling functional programmable protein array for studying protein–protein interactions in malaria parasites
Authors
Gabriela Arévalo-Pinzón
María González-González
Carlos Fernando Suárez
Hernando Curtidor
Javier Carabias-Sánchez
Antonio Muro
Joshua LaBaer
Manuel Alfonso Patarroyo
Manuel Fuentes
Publication date
01-12-2018
Publisher
BioMed Central
Published in
Malaria Journal / Issue 1/2018
Electronic ISSN: 1475-2875
DOI
https://doi.org/10.1186/s12936-018-2414-2

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