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BMC Complementary Medicine and Therapies

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01-12-2021 | Rifampicin | Research

Ethanolic extract of Mucuna pruriens leaves ameliorates carbon tetrachloride and rifampicin-induced hepatotoxicity and nephrotoxicity in wistar albino rat

Authors: Temidayo Ogunmoyole, Ayomide Micheal Ola-Awe, Omotola Grace Fatile

Published in: BMC Complementary Medicine and Therapies | Issue 1/2021

Abstract

Background

Mucuna pruriens (L.) has been used for the treatment of several ailments in folkloric medicine. The present study therefore investigates the hepatoprotective and nephroprotective potentials of its leaves extract with a view to providing a potent alternative in the management of liver and kidney diseases.

Methodology

Forty male albino rats were randomly placed into eight groups comprising five animals each. Animals in group I were administered with the distilled water, while groups II and VI were exposed to CCl₄ and rifampicin respectively. Animals in groups III and IV were initially exposed CCl₄ and treated with 50 and 100 mg/kg bw M. pruriens respectively. Similarly, groups VII and VIII animals were exposed to rifampicin and treated with 50 and 100 mg/kg bw M. pruriens respectively. Animals in group V were treated with 100 mg/kg bw silymarin by oral gavage after an initial exposure to CCl₄. Selected biomarkers of liver and kidney damage were determined in the serum and organs homogenate. Liver and kidney slices of experimental animals were also stained for histopathological examination.

Results

Exposure to CCl₄ and rifampicin respectively resulted in marked distortion in lipid profile, inhibition of antioxidant enzymes and a surge in ALT, AST, ALP, urea, uric acid, bilirubin and creatine kinase. Treatment with M. pruriens extract reversed all deranged biochemical and histopathological parameters in a dose-dependent manner.

Conclusion

Extract of M. pruriens leaves restored deranged biochemical and histopathological parameters in the liver and kidney with similar potency to silymarin. Hence, leaf extract of M. pruriens is a potential hepatoprotective and nephroprotective agent that can be exploited in the management of liver and kidney diseases.

Azab AE, Albasha MO, Elsayed AS. Prevention of nephropathy by some natural sources of antioxidants. Yangtze Med. 2017;1:235–66.

Cichoż-Lach H, Michalak A. Oxidative stress as a crucial factor in liver diseases. World J Gastroenterol. 2014;20(25):8082–91.PubMedPubMedCentral

Shahat AA, Ullah R, Alqahtani AS, Alsaid MS, Husseiny HA, Al-Meanazel O. Hepatoprotective effect of Eriobotrya japonica leaf extract and its various fractions against carbon tetra chloride induced hepatotoxicity in rats. Evid Based Complement Alternat Med. 2018:3782768. https://doi.org/10.1155/2018/3782768.

Ogunmoyole T, Awodooju M, Idowu S, Daramola O. Phyllanthus amarus extract restored deranged biochemical parameters in rat model of hepatotoxicity and nephrotoxicity. Heliyon. 2020:e05670. https://doi.org/10.1016/j.heliyon.2020.e05670.

Anandhi KV, Pakkiyaraj RM, Geraldine P. Protective effect of Chrysion on carbon tetrachloride (CCl₄)-induced tissue injury in male wistar rats. Toxicol Ind Health. 2011;27(10):923–33.PubMed

Chen YJ, Chou P, Hsu CL, Hung JY, Wu J. Lin, fermented citrus lemon reduces liver injury induced by carbon tetrachloride in rats. Evid Based Complement Alternat Med. 2018:1–10.

Yue-Ming W, Sergio CC, Christopher TB, Taoshen C. Pregnane X receptor and drug-induced liver injury expert Opin. Drug Metab Toxicol. 2014;10(11):1521–32. https://doi.org/10.1517/17425255.2014.963555.CrossRef

Tilburt JC, Kaptchuk TJ. Herbal medicine research and global health: an ethical analysis. Bull World Health Organ. 2008;86(8):594–9.PubMedPubMedCentral

Wichtl M. Herbal drugs and phytopharmaceuticals: a handbook for practice on a scientific basis. Boca Raton: CRC press; 2004.

10.

Yarnell E, Abascal K. Dilemmas of traditional botanical research. Herbal Gram. 2002;55:46–54.

11.

Harvey AL. Natural products in drug discovery. Drug Discov Today. 2008;13(19–20):894–901. https://doi.org/10.1016/j.drudis.2008.07.004.CrossRefPubMed

12.

Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect. 2001;109(1):69–75.PubMedPubMedCentral

13.

Rajeshwar Y, Kumar S, Gupta M, Mazumder UK. Studies on in vitro antioxidant activities of methanol extract of Mucuna pruriens (Fabaceae) seeds. Eur Bull Drug Res Firenze. 2005;13(1):31–9.

14.

Vaidya RA, Allorkar SD, Seth AR, Panday SK. The inhibitory effect of Cowhage plant Mucuna pruriens and L-DOPA in chlorpromazine induced hyperprolacteinaemia in man. Neurol India. 1978;26(4):177–8.PubMed

15.

Vaidya AB, Rajagopalan TG, Mankodi NA, Antarkar DS, Tated PS, Purohit AV, et al. Treatment of Parkinson's disease with the cowhage plant- Mucuna pruriens Bak. Neurol India. 1978;26(4):171–6.PubMed

16.

Hussain G, Manyam BV. Mucuna pruriens proves more effective than L-DOPA in Parkinson’s disease animal model. Phytother Res. 1997;11(6):419–23.

17.

Natasha FM, Harshvadan M, Momodou C, Gianni P, Richard W, Roberto C. Could Mucuna pruriens be the answer to Parkinson's disease management in sub-Saharan Africa and other low-income countries worldwide? Parkinsonism Relat Disord. 2020;73:3–7.

18.

Pant MC, Uddin I, Bhardwaj UR, Tewari RD. Blood sugar and total cholesterol lowering effect of glycine Soja, Mucuna pruriens (D.C.) and Dolichos Diflorus (Linn.) seed diets in normal fasting albino rats. Ind J Med Res. 1968;56(12):1808–11.

19.

Gupta M, Mazumder UK, Chakraborti S, Rath N, Bhawal SR. Antiepieptic and anticancer activity of some indigenous plants. Indian J Physiol Allied Sci. 1997;51(2):53–6.

20.

Rajeshwar Y, Gupta M, Mazumder UK. Antitumour activity and in vitro antioxidant status of Mucuna pruriens (Fabaceae) against Ehrlich ascites carcinoma in Swiss albino mice. Iran J Pharmacol Ther. 2005;4(1):46–53.

21.

Rajeshwar Y, Gupta M, Mazumder UK. In vitro lipid peroxidation and antimicrobial activity of M. pruriens seeds. Iran J Pharmacol Ther. 2005;4(1):32–5.

22.

Manyam BV, Dhanasekaran M, Hare TA. Neuroprotective effects of the antiparkinson drug Mucuna pruriens. Phytother Res. 2004;18(9):706–12.PubMed

23.

Poornachandra MN, Salma K, Shivananda BG, Shivananda TN, Dris R. Mucuna pruriens (L.) DC – a novel drug for learning and memory retrieval. J Food Agric Environ. 2005;3(3&4):13–5.

24.

Tsutsui H, Cai X, Hayashi S. Interleukin-1 family cytokines in liver diseases. Mediat Inflamm. 2015:630265. https://doi.org/10.1155/2015/630265.

25.

Dinarello CA. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 2018;281:8–27. https://doi.org/10.1111/imr.12621.CrossRefPubMedPubMedCentral

26.

Mantovani A, Dinarello CA, Molgora M, Garlanda C. Interleukin-1 and related cytokines the regulation of inflammation and immunity. Immunity. 2019;50:778–95. https://doi.org/10.1016/j.immuni.2019.03.012.CrossRefPubMedPubMedCentral

27.

Hishikar R, Shastry S, Shinde S, Gupta SS. Preliminary phytochemical and anti-inflammator activity of seeds of Mucuna pruriens. Indian J Pharmacol. 1981;13(1):97–8.

28.

Lauk L, Galatti EM, Kirjavainen S, Korestieri AM, Trovato A. Analgesic and antipyretic effects of Mucuna pruriens. Int J Pharmacol. 1993;31(3):213–6.

29.

Guerranti R, Aguiyi JC, Neri S, Leoncini R, Pagani R, Marinello E. Proteins from Mucuna pruriens and enzymes from Echis carinatus venom: characterization and cross-reactions. J Biol Chem. 2002;277(19):17072–8.PubMed

30.

Mattenheimer H. Urinary enzyme measurements in the diagnosis of renal disorders. Ann Clin Lab Sci. 1991;11(3):189–201.

31.

Reitman S, Frankel S. Glutamic – pyruvate transaminase assay by colorimetric method. Am J Clin Path. 1957;28:56–60.PubMed

32.

Englehardt A. Measurement of alkaline phosphatase. Aerztl Labor. 1970;16:42.

33.

Trinder H. A simple Turbidimetric method for the determination of serum cholesterol. Ann Din. 1969;6:165.

34.

Tietz NW. Clinical guide to laboratory tests. 3rd ed. Philadelphia: W.B. Saunders; 1995.

35.

Grove TH. Effect of reagent pH on determination of high-density lipoprotein cholesterol by precipitation with sodium phosphotungstate-magnesium. Clin Chem. 1979;25(4):560–4.PubMed

36.

Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972;18:499–502.PubMed

37.

Sinha AK. Colorimetric assay of catalase. Anal Biochem. 1972;47:389–94.PubMed

38.

Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972;247(15):3170–5.PubMed

39.

Beutler ED. Kelly BM improved method for the determination of blood glutathione. J Lab Clin Med. 1963;61:882–90.PubMed

40.

Weichselbaum TE. An accurate and rapid method for the determination of protein in small amount of blood, serum. Am J Clin Pathol. 1995;16:40.

41.

Ohkawa H, Ohishi H, Yagi K. Assay for lipid peroxide in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95:351–8.PubMed

42.

Halliwell B. Biochemistry of oxidative stress. Biochem Soc Trans. 2007;35:1147–50.PubMed

43.

Almokhtar AA, Ata SE, Azab EA, Fawzia AQ. Oxidative stress and antioxidant mechanisms in human body. J Appl Biotechnol Bioeng. 2019;6(1):43–7.

44.

Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong C-W, et al. The role of oxidative stress and antioxidants in liver diseases. Int J Mol Sci. 2015;16:26087–124 https://doi.org/10.3390/ijms161125942.PubMedPubMedCentral

45.

Agbafor KN, Nwachukwu N. Phytochemical analysis and antioxidant property of leaf extracts of Vitex doniana and Mucuna pruriens. Biochem Res Int. 2011;5:459839. https://doi.org/10.1155/2011/459839.CrossRef

46.

Yang Y, Chen J, Yang L, Song J. Combination of metformin and luteolin synergistically protects carbon tetrachloride-induced hepatotoxicity: mechanism involves antioxidant, anti-inflammatory, antiapoptotic, and Nrf2/HO-1signaling pathway. Biofactors. 2019:1–9. https://doi.org/10.1002/biof.1521.

47.

Enechi OC, Ozougwu VEO. Effects of ethanol extract of Mucuna pruriens leaves on lipid profile and serum electrolytes of rats. J Pharm Biol Sci. 2014;9(2):18–23.

48.

Thyaga K, Divya BJ, Suman J, Venkataswamy M. The Traditional uses and Pharmacological Activities of Mucuna Pruriens (L) Dc: A Comprehensive Review. Indo Am J Pharm Res. 2017;7(01).

49.

Mukesh KY, Priyanka P, Prabhat U, Bina P. Phytochemistry and pharmacological activity of Mucuna pruriens: A review; 2017. https://doi.org/10.22377/ijgp.v11i02.916.CrossRef

50.

Ezim OE, Alagbe OV, Idih FM. Antimalarial activity of ethanol extract of Mucuna pruriens leaves on Nk65 Chloroquine sensitive strain of plasmodium berghei. J Complement Altern Med Res. 2021;13(4):1–7.

51.

Gaamoussi F, Israili ZH, Lyoussi B. Hypoglycemic and hypolipidemic effects of an aqueous extract of Chamaerops humilis leaves in obese, hyperglycemic and hyperlipidemic Meriones shawi rats. Pak J Pharm Sci. 2010;23:212–9.PubMed

52.

Subramani S, Casmir CA. Flavonoids and Antioxidant Capacity of Georgia-Grown Vidalia Onions. J Agric Food Chem. 2002. https://doi.org/10.1021/jf020333a.

53.

Oalienfill D, Siddha GS. Could saponins be a useful treatment for hypercholesterolaemia? Eur J Clin Nutr. 1990;44(1):79–88.

54.

Ann FW, Georgios M, Andrew PM, Paul AR. Promising hypotensive effect of hawthorn extract: a randomized double-blind pilot study of mild, essential hypertension. Phytother Res. 2002;16(1):48–54. https://doi.org/10.1002/ptr.947.CrossRef

55.

Jiang X, Zhang H, Mehmood K. Protective effects of Herpetospermum caudigerum extracts against liver injury induced by carbon tetrachloride in mouse. J Biol Regul Homeost Agents. 2018;32:699–704.PubMed

56.

Dutta S, Chakraborty AK, Dey P. Amelioration of CCl₄ induced liver injury in swiss albino mice by antioxidant rich leaf extract of Croton bonplandianus Baill. PLoS One. 2018:e0196411. https://doi.org/10.1371/journal.pone.0196411.

57.

Benjamin LW, Hartmut J. Mechanisms of inflammatory liver injury and drug-induced hepatotoxicity. Curr Pharmacol Rep. 2018;4:346–57.

58.

Muhammad YB, Adam AA, Jamil DU, Lukman OA, Opke JM. Effect of aqueous extract of mucuna pruriens leaves on liver and kidney function markers in wistar albino rats. Am J Innovative Res Appl Sci. 2015;1(10):379–83.

59.

Sylvester EG, Israel EU, Olajumoke AD. The effect of Gongronema latifolium leaf extract on blood biochemical assay in diabetic rats. J Sci Res Rep. 2015;6(7):514–22.

60.

Kota VR, Srivastava S, Singhal SS. Lipid Peroxidation Products in Human Health and Disease. Oxid Med Cell Longev. 2019:Article ID 7147235 https://doi.org/10.1155/2019/7147235.

61.

Sherlock S. Biochemical investigations in liver disease; some correlations with hepatic histology. J Pathol Bacteriol. 1946;58:523–44.PubMed

62.

Hardison WG, Lee FI. Prognosis in acute liver disease of the alcoholic patient. N Engl J Med. 1966;275:61–6.PubMed

63.

Kim WR, Wiesner RH, Therneau TM, et al. Optimal timing of liver transplantation for primary biliary cirrhosis. Hepatology. 1998;28:33–8.PubMed

64.

Devarbhavi H, Singh R, Patil M, Sheth K, Adarsh CK, Balaraju G. Outcome and determinants of mortality in 269 patients with combination anti-tuberculosis drug-induced liver injury. J Gastroenterol Hepatol. 2013;28:161–7.PubMed

65.

Almeras C, Argiles A. The general picture of uremia. Semin Dial. 2009;22:329–33.PubMed

66.

Safi AJ, Mahmood R, Khan MA, Haq AU. Association of Serum Uric Acid with type 2 diabetes mellitus. J Pak Med Ins. 2004;18(1):59–63.

67.

Huda N, Mosadik A, Awal A, Rahman S. Hepatoprotective Activity of Sharbat deenar against carbon tetrachloride induced hepatotoxicity in rats. Int J Pharmacol Pharm Sci. 2014;8(12). https://doi.org/10.5281/zenodo.1316253.

68.

Zhu R, Yajing W, Zhang L, Qinglong G. Oxidative stress and liver disease. Hepatol Res. 2012;42(8):741–9. https://doi.org/10.1111/j.1872-034x.2012.00996.x.CrossRefPubMed

69.

Jadeja RN, Ranjitsinh VD, Srinivas N. Oxidative Stress in Liver Diseases: Pathogenesis, Prevention, and Therapeutics. Oxid Med Cell Long. 2017. https://doi.org/10.1155/2017/8341286.

70.

Sundaram IK, Deepika S, Vignesh S, Shinomol G, Sahabudeen SM. Poly herbal formulation with anti-elastase and anti-oxidant properties for skin anti-aging. BMC Compl Alt Med. 2018;18:33. https://doi.org/10.1186/s12906-018-2097-9.CrossRef

Title: Ethanolic extract of Mucuna pruriens leaves ameliorates carbon tetrachloride and rifampicin-induced hepatotoxicity and nephrotoxicity in wistar albino rat
Authors: Temidayo Ogunmoyole
Ayomide Micheal Ola-Awe
Omotola Grace Fatile
Publication date: 01-12-2021
Publisher: BioMed Central
Keyword: Rifampicin
Published in: BMC Complementary Medicine and Therapies / Issue 1/2021
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-021-03455-3

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Ethanolic extract of Mucuna pruriens leaves ameliorates carbon tetrachloride and rifampicin-induced hepatotoxicity and nephrotoxicity in wistar albino rat

Abstract

Background

Methodology

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methodology

Results

Conclusion

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