Published in:
Open Access 01-12-2013 | Research
RETRACTED ARTICLE: Changes in the electron paramagnetic resonance spectra of albumin-associated spin-labeled stearic acid as a diagnostic parameter of colorectal cancer
Published in: World Journal of Surgical Oncology | Issue 1/2013
Login to get accessAbstract
Background
With more than 940,000 new colorectal cancer cases worldwide each year, there
is no better way for colorectal cancer routine screening. The aim of this study
was to investigate whether the fatty acid binding to albumin is detectably and
significantly altered in colorectal cancer patients when compared with healthy
people, in order to find a better way for colorectal cancer diagnosis.
Methods
One hundred and forty-one patients operatively treated for colorectal cancer
were included in the examination, and 180 healthy people were also enrolled as
controls. Commercial 16-doxyl stearic acid was used as spin probe. Serum albumin
was analyzed by electron paramagnetic resonance (EPR) with spin probe.
Discriminant analysis was carried out using the measured EPR spectra by SPSS
20.0.
Results
Of the original grouped cases, 89.4% were correctly classified. Of the
cross-validated grouped cases, 86.9% were correctly classified. Using Fisher
linear discriminant analysis we were able to develop a mathematical model allowing
for identification of colorectal cancer patients based on five values (both
relative intensity and peak width) which are obtained from the EPR
spectrum.
Conclusions
Cancer-associated alterations to albumin can be assessed by spin-label EPR.
The potential applications for this diagnostic technique are significant and
represent a cost-effective means for screening patients with cancer. Spin probe
for diagnosis of colorectal cancer might be a useful tool and further studies
should take place in order to investigate all stages of colorectal cancer
patients.