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International Ophthalmology
Published in: International Ophthalmology 10/2020

01-10-2020 | Retinoblastoma | Original Paper

Curcumin suppressed proliferation and migration of human retinoblastoma cells through modulating NF-κB pathway

Authors: Ying-Tao Mu, Huan-Huan Feng, Jin-Qiang Yu, Zhen-Kai Liu, Yan Wang, Jie Shao, Ran-Hui Li, De-Kun Li

Published in: International Ophthalmology | Issue 10/2020

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Abstract

Purpose

To study the effect of curcumin on proliferation and invasion of the human retinoblastoma cells and its potential mechanism.

Methods

A cell line of retinoblastoma (WERI-Rb-1) was treated with various concentrations of curcumin (0–40 µM). Cell number was counted with CCK8 kit, and cell migration was assessed using the Transwell assay. Immunoblotting was performed to detect the proteins of metalloproteinase-2 (MMP-2), MMP-9 and vascular endothelial growth factor (VEGF) as well as nuclear translocation of nuclear factor-κB (NF-κB, p65).

Results

Proliferation and migration of WERI-Rb-1 cells were significantly inhibited by curcumin in a concentration-dependent manner (0–40 µM). Protein expressions of MMP-2, MMP-9 and VEGF in the WERI-Rb-1 cells were also significantly inhibited by curcumin in a concentration-dependent manner (0–40 µM). Furthermore, nuclear translocation of NF-κB (p65) was significantly inhibited by curcumin in time-dependent manner (6–24 h).

Conclusion

Curcumin inhibited proliferation and migration of WERI-Rb-1 cells, a cell line of human retinoblastoma, which might be through modulating NF-κB and its downstream proteins including VEGF, MMP-2, and MMP-9.
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Metadata
Title
Curcumin suppressed proliferation and migration of human retinoblastoma cells through modulating NF-κB pathway
Authors
Ying-Tao Mu
Huan-Huan Feng
Jin-Qiang Yu
Zhen-Kai Liu
Yan Wang
Jie Shao
Ran-Hui Li
De-Kun Li
Publication date
01-10-2020
Publisher
Springer Netherlands
Published in
International Ophthalmology / Issue 10/2020
Print ISSN: 0165-5701
Electronic ISSN: 1573-2630
DOI
https://doi.org/10.1007/s10792-020-01406-4

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