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Virology Journal
Published in: Virology Journal 1/2010

Open Access 01-12-2010 | Research

Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae

Authors: Jing Li, Xiao Wang, Juan Liu, Hong Wang, Xiao-Li Zhang, Wei Tang, Yun-Dong Sun, Xin Wang, Xiu-Ping Yu, Wei-Ming Zhao

Published in: Virology Journal | Issue 1/2010

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Abstract

Background

To establish a convenient system for the study of human papillomavirus (HPV), we inserted a Saccharomyces cerevisiae selectable marker, Ura, into HPV58 genome and transformed it into yeast.

Results

HPV58 genome could replicate extrachromosomally in yeast, with transcription of its early and late genes. However, with mutation of the viral E2 gene, HPV58 genome lost its mitotic stability, and the transcription levels of E6 and E7 genes were upregulated.

Conclusions

E2 protein could participate in viral genome maintenance, replication and transcription regulation. This yeast model could be used for the study of certain aspects of HPV life cycle.
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Literature
1.
Doorbar J: The papillomavirus life cycle. J Clin Virol 2005, 32: 7-15. 10.1016/j.jcv.2004.12.006CrossRef
2.
Wu Y, Chen Y, Li L, Yu G, Zhang Y, He Y: Associations of high-risk HPV types and viral load with cervical cancer in China. J Clin Virol 2005, 35: 264-269. 10.1016/j.jcv.2005.07.011PubMedCrossRef
3.
Chan PK, Cheung TH, Li WW, Lo KW, Chan MY, Cheung JL, Cheng AF: Association of Human Papillomavirus Type 58 Variant With the Risk of Cervical Cancer. J Natl Cancer Inst 2002, 94: 1249-1253.PubMedCrossRef
4.
Liaw KL, Hsing AW, Schiffman MH, You SL, Zhang T, Burk R, Chen CJ: Human papillomavirus types 52 and 58 are prevalent in cervical cancers from Chinese women. Int J Cancer 1997, 73: 775-776. 10.1002/(SICI)1097-0215(19971127)73:5<775::AID-IJC27>3.0.CO;2-3PubMedCrossRef
5.
Bielinsky AK, Gerbi SA: Where it all starts: eukaryotic origins of DNA replication. J Cell Sci 2001, 114: 643-651.PubMed
6.
Alves-Rodrigues I, Galão RP, Meyerhans A, Díez J: Saccharomyces cerevisiae: A useful model host to study fundamental biology of viral replication. Virus Research 2006, 120: 49-56. 10.1016/j.virusres.2005.11.018PubMedCrossRef
7.
Angeletti PC, Kim K, Fernandez FJ, Lambert PF: Stable replication of papillomavirus genomes in Saccharomyces cerevisiae. J Virol 2002, 76: 3350-3358. 10.1128/JVI.76.7.3350-3358.2002PubMedPubMedCentralCrossRef
8.
Chattopadhyay A, Schmidt MC, Khan SA: Identification of a 450-bp region of human papillomavirus type 1 that promotes episomal replication in Saccharomyces cerevisiae. Virology 2005, 340: 133-142. 10.1016/j.virol.2005.06.029PubMedCrossRef
9.
Kushinirov VV: Rapid and reliable protein extraction from yeast. Yeast 2000, 16: 857-860. 10.1002/1097-0061(20000630)16:9<857::AID-YEA561>3.0.CO;2-BCrossRef
10.
Kapoor P, Shire K, Frappier L: Reconstitution of Epstein-Barr virus-based plasmid partitioning in budding yeast. EMBO J 2001, 20: 222-230. 10.1093/emboj/20.1.222PubMedPubMedCentralCrossRef
11.
Li J, Liu J, Wang X, Zhao L, Chen Q, Zhao WM: A water bath method for preparation of RNA from Saccharomyces cerevisiae. Analy Bioch 2009, 384: 189-190. 10.1016/j.ab.2008.09.021CrossRef
12.
Rogers AJ, Loggen M, Lee K, Angeletti PC: Varying efficiency of long term replication of papillomaviruses in Saccharomyces cerevisiae. Virology 2008, 381: 6-10. 10.1016/j.virol.2008.08.038PubMedPubMedCentralCrossRef
13.
Hsiao CL, Carbon J: High-frequency transformation of yeast by plasmids containing the cloned yeast. ARG4 gene. Proc Natl Acad Sci 1979, 76: 3829-3833. 10.1073/pnas.76.8.3829PubMedCrossRef
14.
Stinchcomb DT, Struhl K, Davis RW: Isolation and characterisation of a yeast chromosomal replicator. Nature 1979, 282: 39-43. 10.1038/282039a0PubMedCrossRef
15.
Maraherns Y, Stillman B: A yeast chromosomal origin of DNA replication defined by multiple functional elements. Science 1992, 255: 817-823. 10.1126/science.1536007CrossRef
16.
Rabson MS, Yee C, Yang YC, Howley PM: Bovine papillomavirus type 1 3' early region transformation and plasmid maintenance functions. J Virol 1986, 60: 626-634.PubMedPubMedCentral
17.
Ustav M, Ustav E, Szymanski P, Stenlund A: Identification of the origin of replication of bovine papillomavirus and characterization of the viral origin recognition factor E1. EMBO J 1991, 10: 4321-4329.PubMedPubMedCentral
18.
Abroi A, Ilves I, Kivi S, Ustav M: Analysis of chromatin attachment and partitioning functions of bovine papillomavirus type 1 E2 protein. J Virol 2004, 78: 2100-2113. 10.1128/JVI.78.4.2100-2113.2004PubMedPubMedCentralCrossRef
19.
Lehman CW, King DS, Botchan MR: A papillomavirus E2 phosphorylation mutant exhibits normal transient replication and transcription but is defective in transformation and plasmid retention. J Virol 1997, 71: 3652-3665.PubMedPubMedCentral
20.
Kim K, Angeletti PC, Hassebroek EC, Lambert PF: Identification of cis -Acting Elements That Mediate the Replication and Maintenance of Human Papillomavirus Type 16 genomes in Saccharomyces cerevisiae. J Virol 2005, 79: 5933-5942. 10.1128/JVI.79.10.5933-5942.2005PubMedPubMedCentralCrossRef
21.
Pittayakhajonwut D, Angeletti PC: Analysis of cis -elements that facilitate extrachromosomal persistence of human papillomavirus genomes. Virology 2008, 374: 304-314. 10.1016/j.virol.2008.01.013PubMedPubMedCentralCrossRef
22.
Bechtold V, Beard P, Raj K: Human Papillomavirus Type 16 E2 Protein Has No Effect on transcription from episomal viral DNA. J Virol 2003, 77: 2012-2028. 10.1128/JVI.77.3.2021-2028.2003CrossRef
23.
Bouvard V, Storey A, Pim D, Banks L: Characterization of the human papillomavirus E2 protein: evidence of trans-activation and trans-repression in cervical keratinocytes. EMBO J 1994, 13: 5451-5459.PubMedPubMedCentral
24.
Rosenstierne MW, Vinther J, Hansen CN, Prydsoe M, Norrild B: Identification and characterization of a cluster of transcription start sites located in the E6 ORF of human papillomavirus type 16. J Gen Virol 2003, 84: 2909-2920. 10.1099/vir.0.19332-0PubMedCrossRef
25.
Demeret C, Desaintes C, Yaniv M, Thierry F: Different mechanisms contribute to the E2-mediated transcriptional repression of human papillomavirus type18 viral oncogenes. J Virol 1997, 71: 9343-9349.PubMedPubMedCentral
26.
Sanchez IE, Dellarole M, Gaston K, de Prat Gay G: Comprehensive comparison of the interaction of the E2 master regulator with its cognate target DNA sites in 73 human papillomavirus types by sequence statistics. Nucleic Acids Res 2008, 36: 756-769. 10.1093/nar/gkm1104PubMedPubMedCentralCrossRef
Metadata
Title
Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae
Authors
Jing Li
Xiao Wang
Juan Liu
Hong Wang
Xiao-Li Zhang
Wei Tang
Yun-Dong Sun
Xin Wang
Xiu-Ping Yu
Wei-Ming Zhao
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2010
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-7-368

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