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Journal of Cardiovascular Translational Research

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01-06-2019 | Original Article

Release of Mitochondrial and Nuclear DNA During On-Pump Heart Surgery: Kinetics and Relation to Extracellular Vesicles

Authors: Anton Baysa, Anton Fedorov, Kirill Kondratov, Arno Ruusalepp, Sarkis Minasian, Michael Galagudza, Maxim Popov, Dmitry Kurapeev, Alexey Yakovlev, Guro Valen, Anna Kostareva, Jarle Vaage, Kåre-Olav Stensløkken

Published in: Journal of Cardiovascular Translational Research | Issue 3/2019

Abstract

During heart surgery with cardiopulmonary bypass (CPB), the release of mitochondrial (mtDNA) and nuclear DNA (nDNA) and their association to extracellular vesicles were investigated. In patients undergoing elective coronary artery bypass grafting (CABG, n = 12), blood was sampled before, during, and after surgery from peripheral artery, pulmonary artery, and the coronary sinus. Plasma was separated in three fractions: microvesicles, exosomes, and supernatant. mtDNA and nDNA were measured by qPCR. mtDNA and nDNA levels increased after start of surgery, but before CPB, and increased further during CPB. mtDNA copy number was about 1000-fold higher than nDNA. mtDNA was predominantly localized to the vesicular fractions in plasma, whereas nDNA was predominantly in the supernatant. The amount of free mtDNA increased after surgery. There was no net release or disappearance of DNAs across the pulmonary, systemic, or coronary circulation. Extracellular DNAs, in particular mtDNA, may be important contributors to the whole-body inflammation during CPB.

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Title: Release of Mitochondrial and Nuclear DNA During On-Pump Heart Surgery: Kinetics and Relation to Extracellular Vesicles
Authors: Anton Baysa
Anton Fedorov
Kirill Kondratov
Arno Ruusalepp
Sarkis Minasian
Michael Galagudza
Maxim Popov
Dmitry Kurapeev
Alexey Yakovlev
Guro Valen
Anna Kostareva
Jarle Vaage
Kåre-Olav Stensløkken
Publication date: 01-06-2019
Publisher: Springer US
Published in: Journal of Cardiovascular Translational Research / Issue 3/2019
Print ISSN: 1937-5387
Electronic ISSN: 1937-5395
DOI: https://doi.org/10.1007/s12265-018-9848-3

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