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Inflammation Research

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01-01-2017 | Original Research Paper

Regulation of the NLRP3 inflammasome in Porphyromonas gingivalis-accelerated periodontal disease

Authors: Yohei Yamaguchi, Tomoko Kurita-Ochiai, Ryoki Kobayashi, Toshihiko Suzuki, Tomohiro Ando

Published in: Inflammation Research | Issue 1/2017

Abstract

Objective

Porphyromonas gingivalis is involved in the pathogenesis of chronic inflammatory periodontal disease. Recent studies have suggested that the NLRP3 inflammasome plays an important role in the development of chronic inflammation. We investigated a possible association between the inflammasome in gingival inflammation and bone loss induced by P. gingivalis infection using NLRP3-deficient mice.

Methods

Wild-type and NLRP3-deficient mice were injected orally with P. gingivalis. We assessed alveolar bone loss, expression of pro-interleukin (IL)-1β, pro-IL-18, receptor activator of nuclear factor kappa-B ligand (RANKL), and osteoprotegerin (OPG) in gingival tissue, as well as IL-1β, IL-18, and IL-6 production and caspase-1 activity in peritoneal macrophages.

Results

Porphyromonas gingivalis challenge significantly increased alveolar bone loss; gingival gene expression of pro-IL-1β, pro-IL-18, and RANKL; production of IL-1β, IL-18, and IL-6; and caspase-1 activity in peritoneal macrophages of wild-type mice, but did not affect NLRP3-deficient mice. Meanwhile, OPG mRNA expression in gingival tissue and peritoneal IL-6 production were significantly higher in NLRP3-knockout mice.

Conclusions

Porphyromonas gingivalis activated innate immune cells via the NLRP3 inflammasome. These results suggest that the NLRP3 inflammasome, followed by a response from the IL-1 family, is critical in periodontal disease induced by wild-type P. gingivalis challenge via sustained inflammation.

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Title: Regulation of the NLRP3 inflammasome in Porphyromonas gingivalis-accelerated periodontal disease
Authors: Yohei Yamaguchi
Tomoko Kurita-Ochiai
Ryoki Kobayashi
Toshihiko Suzuki
Tomohiro Ando
Publication date: 01-01-2017
Publisher: Springer International Publishing
Published in: Inflammation Research / Issue 1/2017
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-016-0992-4

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Regulation of the NLRP3 inflammasome in Porphyromonas gingivalis-accelerated periodontal disease

Abstract

Objective

Methods

Results

Conclusions

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Abstract

Objective

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Conclusions

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