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Archives of Gynecology and Obstetrics

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01-06-2017 | Gynecologic Oncology

Regulation of NEAT1/miR-214-3p on the growth, migration and invasion of endometrial carcinoma cells

Authors: Jian Wang, Xiangzhai Zhao, Zhaojun Guo, Xiaolin Ma, Yueqing Song, Ying Guo

Published in: Archives of Gynecology and Obstetrics | Issue 6/2017

Abstract

Objective

To investigate the function and mechanism of lnc NEAT1 in regulating the growth, migration and invasion of endometrial carcinoma (EC) cells.

Materials and methods

NEAT1 and miR-214-3p levels were measured by qRT-PCR. The protein levels of HMGA1, β-catenin, c-myc and MMP9 were evaluated by Western blot. The effects of NEAT1, HMGA1, miR-214-3p on the viability, migration and invasion of HEC-1A cells were accessed by WST-1 assay and transwell migration/invasion assay. The effect of miR-214-3p on Wnt signaling activity was tested by luciferase reporter assay.

Results

NEAT1, HMGA1 and β-catenin were significantly upregulated in EC tissues, and miR-214-3p was significantly downregulated. NEAT1 promoted the growth, migration and invasion of HEC-1A cells, and mRNA level of Wnt/β-catenin downstream genes c-myc and MMP9. In addition, HMGA1 upregualted the protein and mRNA levels of Wnt/β-catenin downstream genes c-myc and MMP9, and could improve cell viability, and increase numbers of migration and invasion of HEC-1A cells. miR-214-3p overexpression inhibited the proliferation, migration and invasion of HEC-1A cells, while NEAT1 overexpression reversed these effects. miR-214-3p overexpression inhibited the activity of Wnt/β-catenin pathway, while NEAT1 overexpression reversed this effect. Then, si-HMGA1 reduced the activity of Wnt/β-catenin pathway. Moreover, we found NEAT1 and HMGA1 bound to miR-214-3p by luciferase reporter assay, and NEAT1 and HMGA1 expression were negatively correlated with miR-214-3p.

Conclusion

NEAT1 regulates HMGA1 via miR-214-3p to regulate Wnt/β-catenin pathway, thus promotes the growth, migration and invasion of HEC-1A cells.

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Title: Regulation of NEAT1/miR-214-3p on the growth, migration and invasion of endometrial carcinoma cells
Authors: Jian Wang
Xiangzhai Zhao
Zhaojun Guo
Xiaolin Ma
Yueqing Song
Ying Guo
Publication date: 01-06-2017
Publisher: Springer Berlin Heidelberg
Published in: Archives of Gynecology and Obstetrics / Issue 6/2017
Print ISSN: 0932-0067
Electronic ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-017-4365-1

At a glance: The STEP trials

Springer Medicine

Regulation of NEAT1/miR-214-3p on the growth, migration and invasion of endometrial carcinoma cells

Abstract

Objective

Materials and methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Materials and methods

Results

Conclusion

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