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Archives of Virology
Published in: Archives of Virology 1/2013

01-01-2013 | Brief Report

Recombinant HCMV UL128 expression and functional identification of PBMC-attracting activity in vitro

Authors: Gao Hui-hui, Tao Ran, Zheng Qi, Xu Jun, Shang Shi-qiang

Published in: Archives of Virology | Issue 1/2013

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Abstract

Human cytomegalovirus (HCMV) has evolved several immune evasion strategies. One strategy is controlling the movement of peripheral blood mononuclear cells (PBMCs) by encoding homologues of chemokines. Our aim was to determine whether HCMV open reading frame (ORF) UL128 could encode a protein that attracts PBMCs like a β-chemokine. The recombinant UL128 protein was synthesized by construction of a stably transfected CHO-UL128 cell line, and a chemotaxis assay showed that UL128 was able to attract PBMCs with a potency equal to that of MIP-1α in vitro. We hypothesize that UL128 protein may act as a β-chemokine homologue in viral pathogenesis.
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Metadata
Title
Recombinant HCMV UL128 expression and functional identification of PBMC-attracting activity in vitro
Authors
Gao Hui-hui
Tao Ran
Zheng Qi
Xu Jun
Shang Shi-qiang
Publication date
01-01-2013
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 1/2013
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-012-1378-8

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