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European Journal of Nutrition
Published in: European Journal of Nutrition 5/2015

01-08-2015 | Original Contribution

Rapid down-regulation of hepatic lipid metabolism by phenolic fraction from extra virgin olive oil

Authors: Paola Priore, Donatella Caruso, Luisa Siculella, Gabriele V. Gnoni

Published in: European Journal of Nutrition | Issue 5/2015

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Abstract

Purpose

Regular consumption of extra virgin olive oil (EVOO) is associated with a low incidence of atherosclerotic diseases. The phenolic component contributes to the hypolipidemic action of EVOO, although the biochemical mechanisms leading this beneficial outcome are not fully understood. Since liver plays a pivotal role in the whole body lipid homeostasis, we investigated the short-term effects of EVOO extract, with a high phenol content (HPE), on lipid synthesis in primary rat hepatocytes. Refined olive oil extract, with a low phenol content, was used throughout this study as a control.

Methods

Olive oil phenols isolated with methanolic extractions were subsequently analyzed by high performance liquid chromatography, electrospray ionization tandem mass spectrometry, and gas chromatography mass spectrometry. Rat hepatocytes were obtained from collagenase perfusion of liver. A colorimetric assay was performed to exclude cytotoxicity of the extracts. Radioenzymatic methods were used in order to investigate hepatic lipid metabolism.

Results

HPE, dose- (0.1–50 μg/mL) and time-dependently (0.5–4 h) inhibited both lipogenesis and cholesterogenesis (n = 6, P < 0.05), as well as triglycerides synthesis (n = 5, P < 0.05). We showed that these effects are attributable to a short-term modulation by HPE of the key enzymes implicated in the abovementioned pathways (n = 5, P < 0.05).

Conclusions

The decrease in hepatic lipid synthesis may represent a potential mechanism underlying the hypolipidemic effect of EVOO phenols.
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Metadata
Title
Rapid down-regulation of hepatic lipid metabolism by phenolic fraction from extra virgin olive oil
Authors
Paola Priore
Donatella Caruso
Luisa Siculella
Gabriele V. Gnoni
Publication date
01-08-2015
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Nutrition / Issue 5/2015
Print ISSN: 1436-6207
Electronic ISSN: 1436-6215
DOI
https://doi.org/10.1007/s00394-014-0761-5

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