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European Journal of Clinical Microbiology & Infectious Diseases
Published in: European Journal of Clinical Microbiology & Infectious Diseases 6/2005

01-06-2005 | Concise Article

Rapid detection of methicillin-resistant Staphylococcus aureus from screening enrichment broths by real-time PCR

Authors: K. Levi, K. J. Towner

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 6/2005

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Abstract

The aim of this study was to evaluate real-time PCR (LightCycler) kits for the detection of methicillin-resistant Staphylococcus aureus in enrichment broth using 200 sets of patient screening swab samples. One hundred sets were processed using a lysostaphin/lysozyme extraction method and a further 100 were tested using a column extraction kit. The PCR kits with lysostaphin/lysozyme lysis showed 95.7% sensitivity, 90.8% specificity, and negative and positive predictive values of 98.6% and 75.9%, respectively, compared with 88%, 95.9%, 84.6% and 95.9%, respectively, with the column extraction kit.
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Metadata
Title
Rapid detection of methicillin-resistant Staphylococcus aureus from screening enrichment broths by real-time PCR
Authors
K. Levi
K. J. Towner
Publication date
01-06-2005
Publisher
Springer-Verlag
Published in
European Journal of Clinical Microbiology & Infectious Diseases / Issue 6/2005
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI
https://doi.org/10.1007/s10096-005-1336-4

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Keynote webinar | Spotlight on medication adherence

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WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

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