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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

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Open Access 06-07-2021 | Ranitidine | Original Article

Effects of histamine and various histamine receptor antagonists on gene expression profiles of macrophages during compressive strain

Authors: PD. Dr. rer. nat. Agnes Schröder, Catharina Petring, Dr. med. dent. Anna Damanaki, Professor Dr. med. Jonathan Jantsch, Professor Dr. Dr. Peter Proff, PhD, MD, DDS, PD. Dr. Dr. Christian Kirschneck, PhD, DDS

Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Special Issue 1/2022

Abstract

Purpose

Tissue hormone histamine can accumulate locally within the periodontal ligament via nutrition or may be released during allergic reactions by mast cells, which may have an impact on orthodontic tooth movement. In addition to periodontal ligament fibroblasts, cells of the immune system such as macrophages are exposed to compressive strain. The aim of this study was thus to investigate the impact of histamine on the gene expression profile of macrophages in the context of simulated orthodontic compressive strain.

Methods

Macrophages were incubated with different histamine concentrations (50, 100, 200 µM) for 24 h and then either left untreated or compressed for another 4 h. To assess the role of different histamine receptors, we performed experiments with antagonists for histamine 1 receptor (cetirizine), histamine 2 receptor (ranitidine) and histamine 4 receptor (JNJ7777120) under control and pressure conditions. We tested for lactate dehydrogenase release and analyzed the expression of genes involved in inflammation and bone remodeling by reverse transcription quantitative polymerase chain reaction (RT-qPCR).

Results

Histamine elevated gene expression of tumor necrosis factor under control conditions and in combination with pressure application. Increased prostaglandin-endoperoxide synthase‑2 mRNA was observed when histamine was combined with compressive force. Interleukin‑6 gene expression was not affected by histamine treatment. In macrophages, compressive strain increased osteoprotegerin gene expression. Histamine further elevated this effect. Most of the observed histamine effects were blocked by the histamine 1 receptor antagonist cetirizine.

Conclusions

Histamine has an impact on the gene expression profile of macrophages during compressive strain in vitro, most likely having an impairing effect on orthodontic tooth movement by upregulation of osteoprotegerin expression.

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Title: Effects of histamine and various histamine receptor antagonists on gene expression profiles of macrophages during compressive strain
Authors: PD. Dr. rer. nat. Agnes Schröder
Catharina Petring
Dr. med. dent. Anna Damanaki
Professor Dr. med. Jonathan Jantsch
Professor Dr. Dr. Peter Proff, PhD, MD, DDS
PD. Dr. Dr. Christian Kirschneck, PhD, DDS
Publication date: 06-07-2021
Publisher: Springer Medizin
Keywords: Ranitidine
Cytokines
Cetirizine
Published in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Issue Special Issue 1/2022
Print ISSN: 1434-5293
Electronic ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-021-00318-x

At a glance: The STEP trials

Springer Medicine

Effects of histamine and various histamine receptor antagonists on gene expression profiles of macrophages during compressive strain

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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