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BMC Oral Health
Published in: BMC Oral Health 1/2021

Open Access 01-12-2021 | Research article

Quaternary ammonium silane (k21) based intracanal medicament triggers biofilm destruction

Authors: Esther Sook Kuan Kok, Xian Jin Lim, Soo Xiong Chew, Shu Fen Ong, Lok Yin See, Siao Hua Lim, Ling Ang Wong, Fabian Davamani, Venkateshbabu Nagendrababu, Amr Fawzy, Umer Daood

Published in: BMC Oral Health | Issue 1/2021

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Abstract

Background

Compare antimicrobial efficacy of a quarternary ammonium silane (QAS)/k21 as an intracanal medicament against E. faecalis and C. albicans biofilms formed on root dentin.

Methodology

Dentin blocks were sterilized and E. faecalis and C. albicans microbial colonies were counted for colony-forming-units against 2%k21, 2%CHX and Ca(OH)2 medicaments. Biofilm colonies after 7 days on dentin were analysed using confocal laser scanning microscopy with live/dead bacterial viability staining. TEM was done to study dentin collagen matrix. Dentin discs from 3rd day and 7th day well plate was used for Raman spectra and observed under fluorescent-microscope. Docking studies were carried out on MMP-2 S1 binding-domain with k21.

Results

There was reduction of E. faecalis/C. albicans when k21, chlorhexidine and calcium hydroxide were used with highest percentage in 2%k21 treated specimens. 2%k21 showed dense and regular collagen network with intact cross-banding and decreased Raman intensity for 2%k21 on 3rd day. NaOCl + k21 showed least adherence, whereas saline groups showed highest adherence of E. faecalis and C. albicans to root-canal dentin. Alizarin red staining of hDPSCs revealed calcium deposition in all groups with significant difference seen amongst 2%k21 groups. MMP-2 ligand binding was seen accurately indicating possible target sites for k21 intervention.

Conclusion

2%k21 can be considered as alternative intracanal medicament.
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Metadata
Title
Quaternary ammonium silane (k21) based intracanal medicament triggers biofilm destruction
Authors
Esther Sook Kuan Kok
Xian Jin Lim
Soo Xiong Chew
Shu Fen Ong
Lok Yin See
Siao Hua Lim
Ling Ang Wong
Fabian Davamani
Venkateshbabu Nagendrababu
Amr Fawzy
Umer Daood
Publication date
01-12-2021
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2021
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-021-01470-x

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