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01-02-2018 | Brief Report

Quantitative analysis of senecavirus A in tissue samples from naturally infected newborn piglets

Authors: Alais Maria Dall Agnol, Flavia Megumi Miyabe, Raquel Arruda Leme, Thalita Evani Silva Oliveira, Selwyn Arlington Headley, Amauri Alcindo Alfieri, Alice Fernandes Alfieri

Published in: Archives of Virology | Issue 2/2018

Abstract

In this study, we determined the distribution of senecavirus A (SVA) and viral RNA load in different organs and tissues of naturally infected piglets. A TaqMan-based qRT-PCR assay was performed using RNA extracted from brainstem, cerebellum, cerebrum, heart, kidney, liver, lungs, small intestine, spleen, urinary bladder, and tonsils of seven newborn piglets. SVA was detected in 57 out of 70 tissue samples (81.4%). Viral loads ranged from 4.07 to 10.38 log₁₀ genomic copies per g of tissue. The results show that SVA has tropism for various organs in naturally infected newborn piglets, especially for tonsils, spleen, lungs, and liver. Lymphoid organs had the highest viral loads and may be important sites for SVA replication.

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Title: Quantitative analysis of senecavirus A in tissue samples from naturally infected newborn piglets
Authors: Alais Maria Dall Agnol
Flavia Megumi Miyabe
Raquel Arruda Leme
Thalita Evani Silva Oliveira
Selwyn Arlington Headley
Amauri Alcindo Alfieri
Alice Fernandes Alfieri
Publication date: 01-02-2018
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 2/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-017-3630-8

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