Published in:
01-12-2019 | Brief Report
Quantification of ovine herpesvirus 2 by digital PCR in an outbreak of malignant catarrhal fever
Authors:
Tatiana Flávia Pinheiro de Oliveira, Mateus Laguardia-Nascimento, Fabiana Galtarossa Xavier, Carla do Amaral Pinto, Luciana Rabello Ferreira, Ivy de Castro Campos de Souza, Márcia Elisa Hammerschmitt, Ronaldo Michel Bianchi, Júlia Gabriela Wronski, Rodrigo Nestor Etges, Grazziane Maciel Rigon, Marcelo Fernandes Camargos, Anselmo Vasconcelos Rivetti Júnior, Antonio Augusto Fonseca Junior
Published in:
Archives of Virology
|
Issue 12/2019
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Abstract
Infection with ovine gammaherpesvirus 2 (OvHV-2) is generally asymptomatic in sheep; however, when it crosses the species barrier, it causes malignant catarrhal fever (MCF) in cattle. In the present study, we developed a real-time PCR assay and a droplet digital PCR assay and use both methods to study an outbreak caused by OvHV-2. Both PCR methods showed high sensitivity and specificity and were able to detect low copy numbers of OvHV-2 in sheep and cattle. The present study describes the first digital PCR quantification of OvHV-2 genome copies in samples collected from sheep and cattle.