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BMC Infectious Diseases
Published in: BMC Infectious Diseases 1/2014

Open Access 01-12-2014 | Research article

Pulmonary immune responses to 2009 pandemic influenza A (H1N1) virus in mice

Authors: Jin Lv, Dan Wang, Yan-Hong Hua, Shi-Jia Pei, Jin Wang, Wen-Wei Hu, Xi-Liang Wang, Na Jia, Qi-Sheng Jiang

Published in: BMC Infectious Diseases | Issue 1/2014

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Abstract

Background

Well-characterized mice models will afford a cheaper, easy-handling opportunity for a more comprehensive understanding of 2009 influenza A (H1N1) virus’s pathogenesis potential. We aimed to provide a robust description of pulmonary immune responses in the mice infected by the virus.

Methods

BALB/c mice were inoculated intranasally with A/Beijing/501/2009(H1N1) (BJ501) and A/PR/8/34(H1N1) (PR8) viruses and compared for survival rate, viral replication, and kinetics of pulmonary immune responses.

Results

BJ501 virus replicated less efficiently in the lungs than PR8, and both caused lethal illness in the mice. The transient increases of pulmonary TNF-α 2 days post infection for BJ501 and of INF-γ and IL-10 at 6 days post infection for PR8 were observed. IL-2+ and IL-4+ secreting cells showed significant increase 12 days post infection, while IFN-γ+, IgG+ and IgA+ secreting cells increased 6 days post infection. The different patterns of pulmonary immunological parameters between two viruses were at most seen in IL-6, IL-17 secretion and IgG1/IgG2a ratio.

Conclusions

The BALB/c mouse is evaluated as a good pathogenic model for studying BJ501 2009 H1N1 virus. The work provided some basic and detailed data, which might be referred when further evaluating innate and adapted pulmonary immune responses and local viral load in mice.
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Metadata
Title
Pulmonary immune responses to 2009 pandemic influenza A (H1N1) virus in mice
Authors
Jin Lv
Dan Wang
Yan-Hong Hua
Shi-Jia Pei
Jin Wang
Wen-Wei Hu
Xi-Liang Wang
Na Jia
Qi-Sheng Jiang
Publication date
01-12-2014
Publisher
BioMed Central
Published in
BMC Infectious Diseases / Issue 1/2014
Electronic ISSN: 1471-2334
DOI
https://doi.org/10.1186/1471-2334-14-197

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