Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2019

Open Access 01-12-2019 | Pseudomonas Aeruginosa | Research article

Quercus infectoria gall extracts reduce quorum sensing-controlled virulence factors production and biofilm formation in Pseudomonas aeruginosa recovered from burn wounds

Authors: Akhter Ahmed Ahmed, Fraidoon Abdulqadir Salih

Published in: BMC Complementary Medicine and Therapies | Issue 1/2019

Login to get access

Abstract

Background

Quercus gall extracts’ ability to kill pathogens in vitro and even removal of chronic drug-resistant infections has been reported by several studies. The current investigation is focused on the action of extracts of Quercus infectoria gall in their sub-inhibitory concentrations on the corresponding bacterial behaviours instead of killing them.

Methods

The effect of gall extracts on the quorum sensing (QS) associated virulence of multiple drug resistant Pseudomonas aeruginosa recovered from burns wounds was studied. The influence of different extracts on the production of bacterial virulence and biofilm, and expression of the genes encoding quorum sensing and exotoxin A were investigated. Quorum sensing is a crucial regulator of virulence and biofilm development in Pseudomonas aeruginosa and other medical related microbes.

Results

Experiments to characterise and quantify Q. infectoria gall extracts impact on the quorum sensing networks of P.aeruginosa revealed that the expression of las, rhl, and exotoxin A (ETA) genes levels including the associated virulence were reduced by the extracts at their subinhibitory concentrations.

Conclusions

The obtained results indicated that extracts of Q. infectoria galls fight infections either by their inhibitory constituents, which vigorously eradicate cells or by disruption of the pathogens quorum sensing system through weakening the virulence and bacterial coordination.
Literature
1.
Geddes A. Infection in the twenty-first century: predictions and postulates. J Antimicrob Chemoth. 2000;46:873–7.CrossRef
2.
Bjarnsholt T, Jensen PO, Rasmussen TB, Christophersen L, Calum H, Hentzer M, et al. Garlic blocks quorum sensing and promotes rapid clearing of pulmonary Pseudomonas aeruginosa infections. Microbiology. 2005;151:3873–80.PubMedCrossRef
3.
Rasmussen TB, Bjarnsholt T, Skindersoe ME, Hentzer M, Kristoffersen P, Kote M, et al. Screening for quorum-sensing inhibitors (QSI) by use of a novel genetic system, the QSI selector. J Bacteriol. 2005;187(5):1799–814.PubMedPubMedCentralCrossRef
4.
Musthafa KS, Ravi AV, Annapoorani A, Packiavathy IS, Pandian SK. Evaluation of anti-quorum-sensing activity of edible plants and fruits through inhibition of the N-acyl-homoserine lactone system in Chromobacterium violaceum and Pseudomonas aeruginosa. Chemotherapy. 2010;56(4):333–9.PubMedCrossRef
5.
Bouyahya A, Dakka N, Et-Touys A, Abrini J, Bakri Y. Medicinal plant products targeting quorum sensing for combating bacterial infections. Asia Pac J Trop Med. 2017;10(8):729–43.CrossRef
6.
Rasmussen TB, Givskov M. Quorum sensing inhibitors: a bargain of effects. Microbiology. 2006;152(Pt 4):895–904.PubMedCrossRef
7.
Hong KW, Koh CL, Sam CK, Yin WF, Chan KG. Quorum quenching revisited--from signal decays to signalling confusion. Sensors (Basel). 2012;12(4):4661–96.CrossRef
8.
Yang Q, Scheie AA, Benneche T, Defoirdt T. Specific quorum sensing-disrupting activity (a QSI) of thiophenones and their therapeutic potential. Sci Rep. 2015;5:18033.PubMedPubMedCentralCrossRef
9.
Fu B, Wu Q, Dang M, Bai D, Guo Q, Shen L, et al. Inhibition of Pseudomonas aeruginosa biofilm formation by traditional Chinese medicinal herb Herba patriniae. BioMed Res Intern. 2017;2017:1–10.
10.
Smith RS, Iglewski BH. P. aeruginosa quorum-sensing systems and virulence. Curr Opin Microbiol. 2003;6(1):56–60.PubMedCrossRef
11.
Antunes LC, Ferreira RB, Buckner MM, Finlay BB. Quorum sensing in bacterial virulence. Microbiology. 2010;156(Pt 8):2271–82.PubMedCrossRef
12.
Jadaun V, Prateeksha P, Singh BR, Paliya BS, Upreti DK, Rao CV, et al. Honey enhances the anti-quorum sensing activity and anti-biofilm potential of curcumin. RSC Adv. 2015;5(87):71060–70.CrossRef
13.
Upadhyaya I, Upadhyay A, Kollanoor-Johny A, Darre MJ, Venkitanarayanan K. Effect of plant derived antimicrobials on Salmonella enteritidis adhesion to and invasion of primary chicken oviduct epithelial cells in vitro and virulence gene expression. Intern J Mol Sci. 2013;14(5):10608–25.CrossRef
14.
Asfour HZ. Anti-quorum sensing natural compounds. J Microscopy Ultrastruc. 2018;6(1):1–10.CrossRef
15.
Stone GN, Schonrogge K, Atkinson RJ, Bellido D, Pujade-Villar J. The population biology of oak gall wasps(hymenoptera: Cynipidae). Annu Rev Entomol. 2002;47:633–68.PubMedCrossRef
16.
Hwang JK, Shim JS, Chung JY. Anticariogenic activity of some tropical medicinal plants against Streptococcus mutans. Fitoterapia. 2004;75(6):596–8.PubMedCrossRef
17.
Kaur G, Athar M, Alam MS. Quercus infectoria galls possess antioxidant activity and abrogates oxidative stress-induced functional alterations in murine macrophages. Chem Biol Interact. 2008;171(3):272–82.PubMedCrossRef
18.
Dar MS, Ikram M, Fakouhi T. Pharmacology of Quercus infectoria. J Pharm Sci. 1976;65(121976):1791–4.PubMedCrossRef
19.
20.
Mohammadi-Sichani M, Vajihe K, Dokhaharani SC. Evaluation of biofilm removal activity of Quercus infectoria galls against Streptococcus mutans. Dent Res J. 2016;13:46–51.CrossRef
21.
Harborne JB. Phytochemical methods a guide to modern techniques of plant analysis, third ed. London: Chapman & Hall; 1998.
22.
Tille PM. Bailey & Scott’s diagnostic microbiology. 13th ed. Elsevier/Mosby: China; 2014.
23.
CLSI. Methods for dilution antimicrobial susceptibility tests for Bacteria that grow aerobically; approved standard-ninth edition CLSI document M07-A9. Wayne: Clinical and Laboratory Standards Institute; 2012.
24.
Issac ASV, Palani A, Ramaswamy BR, Shunmugiah KP, Arumugam VR. Antiquorum sensing and antibiofilm potential of Capparis spinosa. Arch Med Res. 2011;42(8):658–68.CrossRef
25.
Kessler E, Safrin M, Olson JC, Ohman DE. Secreted LasA of Pseudomonas aeruginosa is a Staphylolytic protease*. J Biol Chem. 1993;268(10):7503–8.PubMed
26.
Chu W, Zhou S, Jiang Y, Zhu W, Zhuang X, Fu J. Effect of traditional Chinese herbal medicine with Antiquorum sensing activity on Pseudomonas aeruginosa. Evid-Based Complem Altern Med. 2013;2013:1–7.CrossRef
27.
Li H, Li X, Wang Z, Fu Y, Ai Q, Dong Y, et al. Autoinducer-2 regulates Pseudomonas aeruginosa PAO1 biofilm formation and virulence production in a dose-dependent manner. BMC Microbiol. 2015;15:192.PubMedPubMedCentralCrossRef
28.
Deziel E, Comeau Y, Villemur R. Initiation of biofilm formation by Pseudomonas aeruginosa 57RP correlates with emergence of hyperpiliated and highly adherent phenotypic variants deficient in swimming, swarming, and twitching motilities. J Bacteriol. 2001;183(4):1195–204.PubMedPubMedCentralCrossRef
29.
Limban C, Marutescu L, Chifiriuc MC. Synthesis, spectroscopic properties and Antipathogenic activity of new Thiourea derivatives. Molecules. 2011;16:7593–607.PubMedPubMedCentralCrossRef
30.
Al-Shabib NA, Husain FM, Ahmed F, Khan RA, Ahmad I, Alsharaeh E, et al. Biogenic synthesis of zinc oxide nanostructures from Nigella sativa seed: prospective role as food packaging material inhibiting broad-spectrum quorum sensing and biofilm. Sci Rep. 2016;6:36761.PubMedPubMedCentralCrossRef
31.
Schaber JA, Carty NL, McDonald NA, Graham ED, Cheluvappa R, Griswold JA, et al. Analysis of quorum sensing-deficient clinical isolates of Pseudomonas aeruginosa. J Med Microbiol. 2004;53:841–53.PubMedCrossRef
32.
Cotar A, Dinu S, Chifiriuc M-C, Banu O, Iordache C, Larion C, et al. Screening of molecular markers of quorum sensing in Pseudomonas aeruginosa strains isolated from clinical infections. Roum Biotechnol Lett. 2008;13(3):3765–71.
33.
Qin X, Emerson J, Stapp J, Stapp L, Abe P, Burns JL. Use of real-time PCR with multiple targets to identify Pseudomonas aeruginosa and other nonfermenting gram-negative bacilli from patients with cystic fibrosis. J Clin Microbiol. 2003;41(9):4312–7.PubMedPubMedCentralCrossRef
34.
Song KP, Chan TK, Ji ZL, Wong SW. Rapid identification of Pseudomonas aeruginosa from ocular isolates by PCR using exotoxin A-specific primers. Mol Cell Probes. 2000;14(4):199–204.PubMedCrossRef
35.
Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 2001;25(4):402–8.PubMedCrossRef
36.
Hazan R, He J, Xiao G, Dekimpe V, Apidianakis Y, Lesic B, et al. Homeostatic interplay between bacterial cell-cell signaling and iron in virulence. PLoS Pathog. 2010;6(3):1–14.CrossRef
37.
Hentzer M, Wu H, Andersen JB, Riedel K, Rasmussen TB, Bagge N, et al. Attenuation of Pseudomonas aeruginosa virulence by quorum sensing inhibitors. EMBO J. 2003;22(15):3803–15.PubMedPubMedCentralCrossRef
38.
Bassetti M, Vena A, Croxatto A, Righi E, Guery B. How to manage Pseudomonas aeruginosa infections. Drugs in Context. 2018;7:1–18.CrossRef
39.
World Health Organisation: Global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. 2017.
40.
Jiang Q, Chen J, Yang C, Yin Y, Yao K. Quorum sensing: a prospective therapeutic target for bacterial diseases. BioMed Res Intern. 2019;2019:1–15.
41.
Lai BM, Yan HC, Wang MZ, Li N, Shen DS. A common evolutionary pathway for maintaining quorum sensing in Pseudomonas aeruginosa. J Microbiol. 2018;56(2):83–9.PubMedCrossRef
42.
Fothergill JL, Panagea S, Hart CA, Walshaw MJ, Pitt TL, Winstanley C. Widespread pyocyanin over-production among isolates of a cystic fibrosis epidemic strain. BMC Microbiol. 2007;7(45):1–10.
43.
Wang R, Starkey M, Hazan R, Rahme LG. Honey’s ability to counter bacterial infections arises from both bactericidal compounds and QS inhibition. Front Microbiol. 2012;3:144.PubMedPubMedCentral
44.
De Vincenti L, Glasenapp Y, Catto C, Villa F, Cappitelli F, Papenbrock J. Hindering the formation and promoting the dispersion of medical biofilms: non-lethal effects of seagrass extracts. BMC Complem Altern Med. 2018;18(1):168.CrossRef
45.
Costerton JW, Philip S. Stewart, Greenberg EP: bacterial biofilms: a common cause of persistent infections. Science. 1999;284:1318–22.PubMedCrossRef
46.
Hayat S, Sabri AN, McHugh TD. Chloroform extract of turmeric inhibits biofilm formation, EPS production and motility in antibiotic resistant bacteria. J Gen Appl Microbiol. 2017;63(6):325–38.PubMedCrossRef
47.
Mattmann ME, Blackwell HE. Small molecules that modulate quorum sensing and control virulence in Pseudomonas aeruginosa. J Org Chem. 2010;75(20):6737–46.PubMedPubMedCentralCrossRef
48.
Adonizio A, Kong KF, Mathee K. Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa by South Florida plant extracts. Antimicrob Agents Ch. 2008;52(1):198–203.CrossRef
49.
Deziel E, FoL L, Milot S, He J, Mindrinos MN, Tompkins RG, et al. Analysis of Pseudomonas aeruginosa 4-hydroxy-2alkylquinolines (HAQs) reveals a role for 4-hydroxy-2heptylquinoline in cell-to-cell communication PNAS. Proc Natl Acad Sci U S A. 2004;101:1339–44.PubMedPubMedCentralCrossRef
50.
51.
Hessler JL, Kreitman RJ. An early step in Pseudomonas exotoxin action is removal of the terminal lysine residue, which allows binding to the KDEL receptor. Biochemistry. 1997;36:14577–82.PubMedCrossRef
52.
Ahmed AA, Salih FA. Low concentrations of local honey modulate ETA expression, and quorum sensing related virulence in drug-resistant Pseudomonas aeruginosa recovered from infected burn wounds. Iran J Basic Med Sci. 2019;22:1–8.
53.
54.
Reimmann C, Beyeler M, Latifi A, Winteler H, Foglino M, Lazdunski A, et al. The global activator GacA of Pseudomonas aeruginosa PAO positively controls the production of the autoinducer N-butyryl-homoserine lactone and the formation of the virulence factors pyocyanin, cyanide, and lipase. Mol Microbiol. 1997;24(2):309–19.PubMedCrossRef
55.
Evd W, Pretorius JC. Purification and identification of active antibacterial components in Carpobrotus edulis L. J Ethnopharmacol. 2001;76(2001):87–91.
Metadata
Title
Quercus infectoria gall extracts reduce quorum sensing-controlled virulence factors production and biofilm formation in Pseudomonas aeruginosa recovered from burn wounds
Authors
Akhter Ahmed Ahmed
Fraidoon Abdulqadir Salih
Publication date
01-12-2019
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2019
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-019-2594-5

Other articles of this Issue 1/2019

BMC Complementary Medicine and Therapies 1/2019 Go to the issue

Research article

Clinical evidence-guided network pharmacology analysis reveals a critical contribution of β1-adrenoreceptor upregulation to bradycardia alleviation by Shenxian-Shengmai

Research article

The synergistic antitumor effect of Huaier combined with 5-Florouracil in human cholangiocarcinoma cells

Research article

Use of alternative medicine, ginger and licorice among Danish pregnant women – a prospective cohort study

Research article

Anti-borreliae efficacy of selected organic oils and fatty acids

Research article

Comparative efficacy of pharmacological and nonpharmacological treatments for chronic idiopathic constipation in China: a Bayesian network meta-analysis

Research article

Herbal or traditional medicine consumption in a Thai worker population: pattern of use and therapeutic control in chronic diseases