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European Journal of Medical Research
Published in: European Journal of Medical Research 1/2023

Open Access 01-12-2023 | Research

PRP significantly promotes the adhesion and migration of vascular smooth muscle cells on stent material

Authors: Yin-Di Wu, Hong-Jing Jiang, Hao-Hao Zhou, Jian-Yi Xu, Qing Liu, Xu-Heng Sun, Yue-Heng Wu, Zhan-Yi Lin

Published in: European Journal of Medical Research | Issue 1/2023

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Abstract

Background

The adhesion and survival state of cells on scaffold material is a major problem in tissue-engineered blood vessel (TEBV) culture. Platelet-rich plasma (PRP) contains a large amount of biologically active factors and fibrin, which is expected to play an important role in TEBV culture.

Purpose

To combine PRP with cells and scaffold material to promote cell adhesion and biological activity on the scaffold material.

Methods

The adhesion status and migration of SMCs under the optimal concentration suitable for SMC growth and the optimal concentration of PRP were examined by scanning electron microscopy, HE staining, CCK-8 assays, qPCR, WB, and other experimental methods and compared with those under the conventional culture (20% FBS); finally, the effect of PRP on the deposition of ECM in vascular tissue engineering culture was verified by three-dimensional culture.

Results

PRP at 20% is a suitable concentration for SMCs. Compared with the control group, the 20% PRP group had better migration, and the number of SMC adhesions was significantly higher than that of the control group. In addition, collagen deposition in the experimental group was significantly higher than that in the control group.

Conclusion

PRP (20%) can promote SMC adhesion, migration, and collagen deposition on the scaffold material.
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Metadata
Title
PRP significantly promotes the adhesion and migration of vascular smooth muscle cells on stent material
Authors
Yin-Di Wu
Hong-Jing Jiang
Hao-Hao Zhou
Jian-Yi Xu
Qing Liu
Xu-Heng Sun
Yue-Heng Wu
Zhan-Yi Lin
Publication date
01-12-2023
Publisher
BioMed Central
Published in
European Journal of Medical Research / Issue 1/2023
Electronic ISSN: 2047-783X
DOI
https://doi.org/10.1186/s40001-023-01541-5

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