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01-02-2008 | Original Article

Proteomic identification of heat shock protein 27 as a differentiation and prognostic marker in neuroblastoma but not in Ewing’s sarcoma

Authors: Cristina Zanini, Francesco Pulerà, Franco Carta, Giuliana Giribaldi, Giorgia Mandili, Milena Maria Maule, Marco Forni, Franco Turrini

Published in: Virchows Archiv | Issue 2/2008

Abstract

Neuroblastoma (NB) and Ewing’s sarcoma (ES) cell lines were analysed by two-dimensional gel electrophoresis (2-DE) searching for new diagnostic/prognostic markers. Protein expression profiles displayed a high degree of similarity with the exception of marked heat shock protein (HSP) 27 and less marked HSP60 and HSP70 family up-modulations in NB cells. HSP27, which showed peculiar variability in different NB cell preparations, responded to all trans-retinoic acid treatment in NB cells but not in ES cells at gene and protein expression levels. Immunohistochemistry studies showed different behaviours of HSP27 and HSP70 expression in NB and ES biopsies. HSP27 was less expressed, whereas HSP70 was more expressed in the immature areas of NB. HSP27 expression showed positive and statistically significant correlation with favourable prognosis, and HSP27 expression also negatively correlated with increasing aggressiveness of histological type. In ES, both chaperones were expressed without characteristic patterns. Our results suggest that HSP27, after further clinical validations, could be used as a marker of neuronal differentiation in vivo for the assessment of the biological behaviour of NB and for the risk stratification of patients.

Armstrong BK, White E, Saracci R (1992) Principles exposure measurement in epidemiology. Oxford University Press, New York, pp 66–67

Bando Y, Katayama T, Kasai K, Taniguchi M, Tamatani M, Tohyama M (2003) GRP94 (94 kDa glucose-regulated protein) suppresses ischemic neuronal cell death against ischemia/reperfusion injury. Eur J Neurosi 18:829–40CrossRef

Biedler JL, Roffler-Tarlov S, Shachner M, Freedman LS (1978) Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res 38:3751–3757PubMed

Biedler JL, Spengler BA, Chang TD, Ross RA (1988) Transdifferentiation of human neuroblastoma cells results in coordinate loss of neuronal and malignant properties. Prog Clin Biol Res 271:265–276PubMed

Brodeur GM, Maris JM (2002) Neuroblastoma. In: Pizzo PA, Poplack DG (eds) Principles and practice of pediatric oncology. Lippincott Williams & Wilkins, Philadhelphia, pp 895–937

Bruey JM, Ducasse C, Bonniaud P, Ravagnan L, Susin SA, Diaz-Latoud C, Gurbuxani S, Arrigo AP, Kroemer G, Solary E, Garrido C (2000) Hsp27 negatively regulates cell death by interacting with cytochrome c. Nat Cell Biol 2:645–652PubMedCrossRef

Carta F, Demuro PP, Zanini C, Santona A, Castiglia D, D’Atri S, Ascierto PA, Napoletano M, Cossu A, Tavolini B, Turrini F, Manca A, Sini MC Palmieri G, Rozzo AC (2005) Analysis of candidate genes through a proteomics-based approach in primary cell lines from malignant melanomas and their metastases. Melanoma Res 15:235–244PubMedCrossRef

Charette SJ, Lavoie JN, Lambert H, Landry J (2000) Inhibition of Daxx-mediated apoptosis by heat shock protein 27. Mol Cell Biol 20:7602–7612PubMedCrossRef

Cimmino F, Spano D, Capasso M, Zambrano N, Russo R, Zollo M, Iascolan A (2007) Comparative proteomic expression profile in all-trans retinoic acid differentiated neuroblastoma cell line. J Proteome Res 6:2550–2564PubMedCrossRef

10.

Ginsberg JP, Woo SY, Johnson ME, Hicks MJ, Horowitz ME (2002) Ewing’s sarcoma family of tumors: Ewing’s sarcoma of bone and soft tissue and the peripheral primitive neuroectodermal tumors. In: Pizzo PA, Poplack DG (eds) Principles and practice of pediatric oncology. Lippincott Williams & Wilkins, Philadhelphia, pp 937–1016

11.

Giribaldi G, Ulliers D, Mannu F, Arese P, Turrini F (2001) Growth of Plasmodium falciparum induces stage-dependent haemichrome formation, oxidative aggregation of band 3, membrane deposition of complement and antibodies, and phagocytosis of parasitized erythrocytes. Br J Haematol 13:492–499CrossRef

12.

Harris MN, Ozpolat B, Abdi F, Gu S, Legler A, Mawuenyega KG, Tirado-Gomez M, Lopez-Berestein G, Chen X (2004) Comparative proteomic analysis of all-trans-retinoic acid treatment reveals systematic posttranscriptional control mechanisms in acute promyelocytic leukemia. Blood 104:1314–1323PubMedCrossRef

13.

Hasegawa SL, Davison JM, Rutten A, Fletcher JA, Fletcher CD (1998) Primary cutaneous Ewing’s sarcoma: immunophenotypic and molecular cytogenetic evaluation of five cases. Am J Surg Pathol 22:310–8PubMedCrossRef

14.

Hoang AT, Huang J, Rudra-Ganguly N, Zheng J, Powell WC, Rabindran SK, Wu C, Roy-Burman P (2000) A novel association between the human heat shock transcription factor 1 (HSF1) and prostate adenocarcinoma. Am J Pathol 156:857–864PubMed

15.

Ishiguro Y, Kato K, Akatsuka H, Iwata H, Nagaya M (1997) Chemotherapy-induced expression of alpha B-crystallin in neuroblastoma. Med Pediatr Oncol 29:11–5PubMedCrossRef

16.

Jolly C, Morimoto RI (2000) Role of the heat shock response and molecular chaperones in oncogenesis and cell death. J Natl Cancer Inst 92:1564–1572PubMedCrossRef

17.

Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402–408PubMedCrossRef

18.

Miyazaki T, Kato H, Faried A, Sohda M, Nakajima M, Fukai Y, Masuda R, Fukuchi M, Ojima H, Tsukada K, Kuwano H (2005) Predictors of response to chemo-radiotherapy and radiotherapy for esophageal squamous cell carcinoma. Anticancer Res 25:2749–2755PubMed

19.

Oakley BR, Kirsch DR, Morris NR (1980) A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem 105:361–363PubMedCrossRef

20.

Peuchmaur M, d’Amore SG, Joshi VV, Hata J, Roald B, Dehner LP, Gerbing RB, Stram DO, Lukens JN, Matthay KK, Shimada H (2003) Revision of the International Neuroblastoma Pathology Classification: confirmation of favorable and unfavorable prognostic subsets in ganglioneuroblastoma, nodular. Cancer 98:2274–2281PubMedCrossRef

21.

Ponthan F, Borgstrom P, Hassan M, Wassberg E, Redfem CP, Kogner P (2001) The vitamin A analogues: 13-cis retinoic acid, 9-cis retinoic acid, and Ro 13-6307 inhibit neuroblastoma tumour growth in vivo. Med Pediatr Oncol 36:127–131PubMedCrossRef

22.

Rane MJ, Pan Y, Singh S, Powell DW, Wu R, Cummins T, Chen Q, McLeish KR, Klein JB (2003) Heat shock protein 27 controls apoptosis by regulating Akt activation. J Biol Chem 278:27828–27835PubMedCrossRef

23.

Rettig WJ, Splenger BA, Chesa PG, Old LJ, Biedler JL (1987) Coordinate changes in neuronal phenotype and surface antigen expression in human neuroblastoma cell variants. Cancer Res 47:1383–1389PubMed

24.

Rocchi P, Ferreri AM, Simone G, Prodi G (1987) Epirubicin-induced differentiation of human neuroblastoma cells in vitro. Anticancer Res 7:247–250PubMed

25.

Sarto C, Valsecchi C, Magni F, Tremolada L, Arizzi C, Cordani N, Cesellato S, Doro G, Favoni P, Perego RA, Raimondo F, Ferrero S, Mocarelli P, Galli-Kienle M (2004) Expression of heat shock protein 27 in human renal cell carcinoma. Proteomics 4:2252–2260PubMedCrossRef

26.

Schlesinger HR, Gerson JM, Moorhead PS, Maguire H, Hummeler K (1976) Establishment and characterization of human neuroblastoma cell lines. Cancer Res 36:3094–3100PubMed

27.

Schwab M, Shimada H, Joshi V, Brodeur GM (2000) Neuroblastic tumours of adrenal gland and sympathetic nervous system. In: Kleihues P, Canavee WK (eds) Pathology and genetics of tumours of the nervous system. IARC, Lyon, pp 153–161

28.

SIOP (2004) Europe Neuroblastoma AIEOP LNESG2 Study: guidelines for the treatment of patients with localized resectable neuroblastoma and analysis of prognostic factors. Ist. G. Gaslini

29.

Thanner F, Sutterlin MW, Kapp M, Rieger L, Morr AK, Kristen P, Dietl J, Gassel AM, Muller T (2005) Heat shock protein 27 is associated with decreased survival in node-negative breast cancer patients. Anticancer Res 25:1649–1653PubMed

30.

Thiele CJ, McKeon C, Triche TJ, Ross RA Reynolds CP, Israel MA (1987) Differential protooncogene expression characterizes histopathologically indistinguishable tumors of the peripheral nervous system. J Clin Invest 80:804–811PubMedCrossRef

31.

Thomas X, Campos L, Mounier C, Cornillon J, Flandrin P, Le QH, Piselli S, Guyotat D (2005) Expression of heat-shock proteins is associated with major adverse prognostic factors in acute myeloid leukemia. Leuk Res 29:1049–1058PubMedCrossRef

32.

Triche T, Askin FB, Kissane JM (1986) Neuroblastoma, Ewing Sarcoma and the differential diagnosis of small- round- blue-cell tumor. In: Finegold M (ed) Pathology of neoplasia in children and adolescents. Sauders, Philadelphia, pp 145–195

33.

Ungar DR, Hailat N Strahler JR, Kuick RD, Brodeur GM, Seeger RC, Reynolds CP, Hanash SM (1994) Hsp27 expression in neuroblastoma: correlation with disease stage. J Nat Can Inst 86:780–4CrossRef

34.

Ushigome S, Machinami R, Soresens PH (2002) Ewing sarcoma/primitive neuroectodermal tumour (PNET). In: Fletcher CDM, Unni KK, Mertens F (eds) Pathology and genetics of tumours of soft tissue and bone. IARC, Lyon, pp 298–300

35.

Voight A, Zintl F (2003) Effects of retinoic acid on proliferation, apoptosis, cytotoxicity, migration, and invasion of neuroblastoma cells. Med Pediatr Oncol 40:205–213CrossRef

36.

Woolson RF, Clarke WR (2002) Statistical methods for the analysis of biomedical data, 2nd edn. Wiley, New York, pp 226–232, 286–288

37.

Yenari MA (2002) Heat shock proteins and neuroprotection. Adv Exp Med Biol 513:281–289PubMed

38.

Zanini C, Giribaldi G, Mandili G, Carta F, Crescenzio N, Bisaro B, Doria A, Foglia L, di Montezemolo LC, Timeus F, Turrini F (2007) Inhibition of heat shock proteins (HSP) expression by quercetin and differential doxorubicin sensitization in neuroblastoma and Ewing’s sarcoma cell lines. J Neurochem 103:1344–1354PubMedCrossRef

Title: Proteomic identification of heat shock protein 27 as a differentiation and prognostic marker in neuroblastoma but not in Ewing’s sarcoma
Authors: Cristina Zanini
Francesco Pulerà
Franco Carta
Giuliana Giribaldi
Giorgia Mandili
Milena Maria Maule
Marco Forni
Franco Turrini
Publication date: 01-02-2008
Publisher: Springer-Verlag
Published in: Virchows Archiv / Issue 2/2008
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-007-0549-6

At a glance: The STEP trials

Springer Medicine

Proteomic identification of heat shock protein 27 as a differentiation and prognostic marker in neuroblastoma but not in Ewing’s sarcoma

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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