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Medical Oncology
Published in: Medical Oncology 4/2018

01-04-2018 | Original Paper

Protective effects of purple carrot extract (Daucus carota) against rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide

Authors: Glaucia Resende Soares, Carolina Foot Gomes de Moura, Marcelo Jose Dias Silva, Wagner Vilegas, Aline Boveto Santamarina, Luciana Pellegrini Pisani, Debora Estadella, Daniel Araki Ribeiro

Published in: Medical Oncology | Issue 4/2018

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Abstract

The aim of this study was to evaluate the chemopreventive potential of purple carrot extract following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO). For this purpose, histopathological analysis, proliferative status, antioxidant activity and inflammatory status were investigated in this setting. A total of 20 male rats were distributed into four groups as follows (n = 5 per group): Group 1—free access to water and commercial diet for 12 weeks; Group 2—received 4NQO at 50 ppm dose in drinking water daily and commercial diet for 12 weeks; Group 3—free access to water and received diet supplemented with purple carrot extract (0.1 g/kg) for 12 weeks; and Group 4—received 4NQO at 50 ppm dose in drinking water daily and diet supplemented with purple carrot extract (0.1 g/kg) for 12 weeks. Histopathological analysis revealed that animals treated with purple carrot extract reduced the oral lesions such as dysplasia and squamous cell carcinoma. Animals with oral pre-neoplastic lesions and treated with purple carrot extract decreased ki-67 and 8-OHdG immunoexpression. Moreover, pNFκBp50 and MyD88 protein expressions were decreased after purple carrot treatment associated or not with 4NQO exposure. SOD-Mn mRNA levels increased with treatment with purple carrot extract as well. In conclusion, our results demonstrated that purple carrot extract was able to protect oral lesions induced by 4NQO in Wistar rats as a result of antioxidant activity, anti-inflammatory potential and antiproliferative and antimutagenic actions.
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Metadata
Title
Protective effects of purple carrot extract (Daucus carota) against rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide
Authors
Glaucia Resende Soares
Carolina Foot Gomes de Moura
Marcelo Jose Dias Silva
Wagner Vilegas
Aline Boveto Santamarina
Luciana Pellegrini Pisani
Debora Estadella
Daniel Araki Ribeiro
Publication date
01-04-2018
Publisher
Springer US
Published in
Medical Oncology / Issue 4/2018
Print ISSN: 1357-0560
Electronic ISSN: 1559-131X
DOI
https://doi.org/10.1007/s12032-018-1114-7

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