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Open Access 01-12-2014 | Research

Prognostic significance of WT1 expression in soft tissue sarcoma

Authors: Ahrong Kim, Eun Young Park, Kyungbin Kim, Jeong Hee Lee, Dong Hoon Shin, Jee Yeon Kim, Do Youn Park, Chang Hun Lee, Mee Young Sol, Kyung Un Choi, Jeung Il Kim, In Sook Lee

Published in: World Journal of Surgical Oncology | Issue 1/2014

Abstract

Background

Soft tissue sarcomas (STS) are rare. We evaluated the WT1 protein expression level in various types of STS and elucidated the value of WT1 as a prognostic factor and a possible therapeutic target.

Methods

Immunohistochemical staining for WT1 was performed in 87 cases of STS using formalin-fixed, paraffin-embedded blocks. The correlation between WT1 expression and clinicopathological factors was analyzed. Survival analysis was conducted in 67 patients. We assessed the validity of WT1 immunohistochemistry as an index of WT1 protein expression using Western blot analysis.

Results

WT1 expression was noted in 47 cases (54.0%). Most rhabdomyosarcomas and malignant peripheral nerve sheath tumors showed WT1 expression (91.7% and 71.4%, respectively; P = 0.005). WT1 expression was related to higher FNCLCC histologic grade and AJCC tumor stage. In the group with high grade STS, strong WT1 expression was correlated with better survival (P = 0.025). The immunohistochemical results were correlated quantitatively with the staining score and the concentration of the Western blot band.

Conclusions

This study demonstrates that various types of STS show positive immunostaining for WT1 and that WT1 expression has a prognostic significance. So STS should be considered candidates for WT1 peptide--based immunotherapy.

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Title: Prognostic significance of WT1 expression in soft tissue sarcoma
Authors: Ahrong Kim
Eun Young Park
Kyungbin Kim
Jeong Hee Lee
Dong Hoon Shin
Jee Yeon Kim
Do Youn Park
Chang Hun Lee
Mee Young Sol
Kyung Un Choi
Jeung Il Kim
In Sook Lee
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: World Journal of Surgical Oncology / Issue 1/2014
Electronic ISSN: 1477-7819
DOI: https://doi.org/10.1186/1477-7819-12-214

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Prognostic significance of WT1 expression in soft tissue sarcoma

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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