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Annals of Surgical Oncology
Published in: Annals of Surgical Oncology 13/2012

01-12-2012 | Breast Oncology

Prognostic Significance of cyclin D1, β-catenin, and MTA1 in Patients with Invasive Ductal Carcinoma of the Breast

Authors: Chun-Wen Cheng, PhD, Yu-Fan Liu, PhD, Jyh-Cherng Yu, MD, Hsiao-Wei Wang, MSc, Shian-Ling Ding, PhD, Chia-Ni Hsiung, MSc, Huan-Ming Hsu, MD, Jia-Ching Shieh, PhD, Pei-Ei Wu, MSc, Chen-Yang Shen, PhD

Published in: Annals of Surgical Oncology | Issue 13/2012

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Abstract

Background

To investigate markers for predicting breast cancer progression, we performed a candidate gene-based study that assessed expression change of three genes, cyclin D1, β-catenin, and metastasis-associated protein-1 (MTA1), involving in aggressive phenotypes of cancerous cells, namely hyperproliferation, epithelial-mesenchymal transition, and global transcriptional regulation.

Methods

Specimens were from 150 enrolled female patients, with invasive ductal carcinoma, followed up for more than 10 years. mRNA expression of cyclin D1, β-catenin, and MTA1 in cancerous and noncancerous cells microdissected from the primary tumor site was determined by quantitative real-time PCR. The relationship between mRNA expression levels of the genes and clinicopathologic features was assessed by statistical analysis. Disease-free and overall survival (DFS and OS) were analyzed by Kaplan–Meier analysis with log-rank test and a multivariate Cox regression model.

Results

Cyclin D1 was shown to be overexpressed in late-stage breast cancer (stage III/IV). Breast cancer with lymph node metastasis (LNM) showed significantly higher frequency of overexpressed cyclin D1, β-catenin, and MTA1 (P < 0.05). Patients carrying greater numbers of overexpressed genes had joint effects on increased risk in tumors of advanced stages (P trend  = 0.03) and LNM (P trend  < 0.01). In the LNM-negative group, patients whose tumors with greater number of cyclin D1, β-catenin, and MTA1 overexpressions were associated with poorer clinical outcomes, with hazard ratio of 14.79 for OS (P = 0.015) and 7.54 for DFS (P = 0.015) using multivariate Cox regression analysis during the 10-year follow-up.

Conclusions

Higher expression of cyclin D1, β-catenin, and MTA1 mRNAs in breast cancers may prove effective in predicting unfavorable outcomes of breast cancer.
Appendix
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Metadata
Title
Prognostic Significance of cyclin D1, β-catenin, and MTA1 in Patients with Invasive Ductal Carcinoma of the Breast
Authors
Chun-Wen Cheng, PhD
Yu-Fan Liu, PhD
Jyh-Cherng Yu, MD
Hsiao-Wei Wang, MSc
Shian-Ling Ding, PhD
Chia-Ni Hsiung, MSc
Huan-Ming Hsu, MD
Jia-Ching Shieh, PhD
Pei-Ei Wu, MSc
Chen-Yang Shen, PhD
Publication date
01-12-2012
Publisher
Springer-Verlag
Published in
Annals of Surgical Oncology / Issue 13/2012
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI
https://doi.org/10.1245/s10434-012-2541-x

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