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Virology Journal
Published in: Virology Journal 1/2010

Open Access 01-12-2010 | Short report

Production and purification of VP2 protein of porcine parvovirus expressed in an insect-baculovirus cell system

Authors: Hongchao Zhou, Guizhe Yao, Shangjin Cui

Published in: Virology Journal | Issue 1/2010

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Abstract

The porcine parvovirus (PPV) VP2 protein was expressed in an insect-baculovirus cell system and was purified using Ni-NTA affinity column chromatography. The recombinant 6-His-tagged VP2 protein with molecular mass (Mr) of about 64 kDa was detected by anti-his antibody and anti-PPV serum. Electron microscopy showed that the purified VP2 protein assembled into spherical particles with diameters ranging from 20 to 22 nm. The expressed VP2 was antigenically similar to the native capsid protein according to HA and a Western blotting assay performed with polyclonal antibodies collected from an outbreak of PPV in one farm. This study provides a foundation for the application of VP2 protein in the clinical diagnosis of PPV or in the vaccination against PPV in the future.
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Metadata
Title
Production and purification of VP2 protein of porcine parvovirus expressed in an insect-baculovirus cell system
Authors
Hongchao Zhou
Guizhe Yao
Shangjin Cui
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Virology Journal / Issue 1/2010
Electronic ISSN: 1743-422X
DOI
https://doi.org/10.1186/1743-422X-7-366

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