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Journal of Ophthalmic Inflammation and Infection
Published in: Journal of Ophthalmic Inflammation and Infection 1/2017

Open Access 01-12-2017 | Original research

Prevention of Bacterial Biofilm Formation on Soft Contact Lenses Using Natural Compounds

Authors: Amira M. El-Ganiny, Ghada H. Shaker, Abeer A. Aboelazm, Heba A. El-Dash

Published in: Journal of Ophthalmic Inflammation and Infection | Issue 1/2017

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Abstract

Purpose

In eye care field, contact lenses (CL) have a great impact on improving vision, but their use can be limited by ocular infection. CL- associated infections can be reduced by good attention to CL storage case practice. CL-care solutions should be able to control microbial growth on CL.
The aim of the study was to evaluate and compare the efficacy of CL-care solutions (found in Egyptian market) with some natural compounds in removal and inhibition of bacterial biofilm formed on soft CL.
Clinical isolates were recovered from patients having conjunctivitis from Benha University Hospital and identified microbiologically. Quantification of biofilm was done using microtiter plate assay. Three multipurpose CL-care solutions were examined for their ability to remove and inhibit biofilm. Also four natural extracts having antibacterial activity and are safe on eye were tested for their anti-biofilm activity.

Results

The major bacterial isolates from eye infections were Pseudomonas aeruginosa (36%) and Staphylococcus spp. (37.8%). Only 33.3% of isolates showed ability to produce weak to moderate biofilm. The tested multi-purpose CL-care solutions showed moderate ability to remove preformed biofilm. Among the tested natural compounds, Calendula officinalis and Buddleja salviifolia extracts showed an excellent efficacy in inhibition of biofilm and also removal of preformed biofilm.

Conclusion

This study demonstrated that isolates from infected eye and CL-cases showed weak to moderate biofilm formation. Calendula officinalis and Buddleja salviifolia extracts showed excellent effect on inhibition and removal of biofilm, these extracts could be added into CL-care solutions which could markedly reduce eye-infections during CL-wear.
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Metadata
Title
Prevention of Bacterial Biofilm Formation on Soft Contact Lenses Using Natural Compounds
Authors
Amira M. El-Ganiny
Ghada H. Shaker
Abeer A. Aboelazm
Heba A. El-Dash
Publication date
01-12-2017
Publisher
Springer Berlin Heidelberg
Published in
Journal of Ophthalmic Inflammation and Infection / Issue 1/2017
Electronic ISSN: 1869-5760
DOI
https://doi.org/10.1186/s12348-017-0129-0

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