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Medical Microbiology and Immunology
Published in: Medical Microbiology and Immunology 3-4/2018

01-08-2018 | Original Investigation

Prevalence and genetic diversity of norovirus genogroup II in children less than 5 years of age with acute gastroenteritis in Tehran, Iran

Authors: Mahsa Farsi, Fatemeh Roodbari, Behrooz Nejati, Arash Arashkia, Somayeh Jalilvand, Alireza Nateghian, Aliakbar Rahbarimanesh, Sayed Mahdi Marashi, Zabihollah Shoja

Published in: Medical Microbiology and Immunology | Issue 3-4/2018

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Abstract

Viral gastroenteritis is a major public health problem worldwide. In Iran, very limited studies have been performed with regard to the epidemiology of noroviruses. This study aimed to evaluate the prevalence and molecular epidemiology of GII noroviruses in hospitalized children less than 5 years of age with acute gastroenteritis (AGE). A total of 210 stool specimens were collected from Ali Asghar Children’s Hospital and Bahrami Children’s Hospital in Tehran, from June 2015 to June 2016. The samples were screened by real-time RT-PCR for genogroup II (GII). Positive samples were genotyped by semi-nested PCR followed by Sanger sequencing and phylogenetic analysis. Norovirus was identified in 36 (17.1%) of 210 specimens. Based on genetic analysis of RdRp and capsid sequences, the strains were clustered into eight RdRp–capsid genotypes: GII.P4–GII.4 Sydney_2012 (41.7%), GII.Pe–GII.4 Sydney_2012 (30.6%), GII.P21–GII.3 (13.9%), GII.P16–GII.4 Sydney_2012 (2.8%), GII.P16–GII.12 (2.8%), GII.P2–GII.4 Sydney_2012 (2.8%), GII.P7–GII.7 (2.8%) and GII.P2–GII.2 (2.8%). We determined several different co-circulating norovirus genotypes in children < 5 years of age with AGE in our hospital in Tehran, Iran. Continued molecular surveillance of noroviruses, including typing of both RdRp and capsid genes, is important for monitoring emerging strains in our continued efforts to reduce the overall burden of norovirus disease.
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Metadata
Title
Prevalence and genetic diversity of norovirus genogroup II in children less than 5 years of age with acute gastroenteritis in Tehran, Iran
Authors
Mahsa Farsi
Fatemeh Roodbari
Behrooz Nejati
Arash Arashkia
Somayeh Jalilvand
Alireza Nateghian
Aliakbar Rahbarimanesh
Sayed Mahdi Marashi
Zabihollah Shoja
Publication date
01-08-2018
Publisher
Springer Berlin Heidelberg
Published in
Medical Microbiology and Immunology / Issue 3-4/2018
Print ISSN: 0300-8584
Electronic ISSN: 1432-1831
DOI
https://doi.org/10.1007/s00430-018-0541-6

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