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01-05-2018 | Original Article

Prevalence and antifungal susceptibility of mycotic agents isolated from skin lesions of horses in Enugu State, Nigeria

Authors: Chioma Inyang Aneke, Iniobong Chukwuebuka Ikenna Ugochukwu, Josephine Okafor, Kennedy Foinkfu Chah

Published in: Comparative Clinical Pathology | Issue 3/2018

Abstract

The study was conducted at Obollo-Afor horse market to determine the occurrence and the antifungal susceptibility profile of mycotic agents in skin lesions of trade horses. The samples were collected by plucking the hair and scraping the epidermal scales using sterile scapel blades. Each sample was planted onto the surface of Sabouraud dextrose agar (SDA) media plate. Inoculated plates was incubated at 25–30 °C and examined daily for fungal growth for 4 weeks. After obtaining pure cultures, they were subcultured on the Potato dextrose agar (PDA) for proper sporulation. Identification of each of the fungal isolates were based on cultural characteristics, microscopic examinations, slide culture technique and biochemical tests. 150 (30.18%) of the examined horses had skin lesions, 97 (64.7%) of which were males while 53 (35.3%) were females. In the wet season, 140 (93.3%) of the horses with skin lesions were observed and 10 (6.6%) were observed to have lesions during the dry season. The overall isolation rates were as follows the genera Aspergillus (24.5%), Trichophyton (14.3%), Microsporum (6.1%), Penicillium (8.2%), Curvularia (6.1%) and Fusarium (4.1%) from skin lesions of horses. All the isolates apart of those of Penicillium spp. were resistant to fluconazole while none of the respective isolates were resistant to voriconazole and nystatin. This study has provided baseline information which includes sex and seasonal distributions of the fungal agents associated with skin lesions in trade horses in Obollo-Afor area of Enugu State. Antifungal susceptibility results shows that voriconazole and nystatin are the drugs of choice in the treatment of dermatomycoses of horses in Nigeria.

Al-Ani FK (1999) Epizootic lymphangitis in horses; a review of the literature. Sci Tech Rev Off Int Epizoot 18:691–699CrossRefPubMed

Alexander BD, Perfect JP (1997) Antifungal resistance trends toward the year 2000. Implications for therapy and new approaches. Drugs 54(5):657–678. https://doi.org/10.2165/00003495-199754050-00002 CrossRefPubMed

Al-Rubiay KK, Al-Rubiay LK (2006) Dermatoepidemiology. A household survey among two urban areas in Basra, city Iraq. Int J Dermatol 4:1–4

Andriole VT (2000) Current and future antifungal therapy. New target for antifungal therapy. Int J Antimicrob Agnts 16(3):317–322. https://doi.org/10.1016/S0924-8579(00)00258-2 CrossRef

Baron EJ, Murray PR, Jorgensen JH, Pfaller MA, Yolken RH (2003) Manual of clinical microbiology, 8th edn. SM press, Washington

Bernado F, Lanca AM, Guerra M, Martins HM (2005) Dermatophytes isolated from pet dogs and cats in Lisbon, Portugal (2000-2004). Rep Port Coll Vet 100(553–554):85–88

Blench R, Chapman R, Slaymeker T (2004) A study of the role of livestock in poverty reduction. Strat Pap 1:1–57

Blocksdorf K (2005) How long do horses live. http://horses.about.com/od/understandinghorses/qt/horseage.html. Accessed 3rd February 2014

Bodey GP (1993) Antifungal agents. In: Bodey GP (ed) Candidiasis, pathogenesis, diagnosis and treatment. Raven Press, New York, pp 371–406

Brilhante RSN, Cordeiro RA, Gomes JMF, Sidrim JJC, Rocha MFG (2003) Canine dermatophytosis caused by an anthropophilic species: molecular and phenotypical characterization of Trichophyton tonsurans. J Med Microbiol 55:1583–1586CrossRef

Bush K, Marczak J (2005) The principles of teaching riding: the official manual of British riding schools. David & Charles, London, London

Cafarchia C (2013) Fungal diseases of horses. Vet Microbiol 167(1-2):215–234. https://doi.org/10.1016/j.vetmic.2013.01.015 CrossRefPubMed

Calderone RA (1989) Immunoregulation of dermatophytosis. Crit Rev Microbiol 16:338–368CrossRef

Center for Food Security and Public Health (CFSPH) (2013) Dermatophytosis: ringworm, tinea and dermatomycosis. College of Veterinary Medicine, Iowa State University, Ames

Chah KF, Majiagbe KA, Kazeem HM, Ezeanyika O, Agbo IC (2012) Dermatophytes from skin lesions of domestic animals in Nsukka, Enugu State, Nigeria. Vet Dermatol 23(6):522–e104. https://doi.org/10.1111/j.1365-3164.2012.01089.x CrossRefPubMed

Cormican MG, Pfaller MA (1996) Standardization of antifungal susceptibility of testing. J Antimicrob Chemoth 38(4):561–578. https://doi.org/10.1093/jac/38.4.561 CrossRef

Edwards EH (1994) The Encyclopaedia of the horse. Dorling Kindersley, London

Ellis D, Stephen D, Helen A, Rosemary H, Robyn B (2000) Descriptions of Medical Fungi, 2nd edn. Mycology Unit, Women‘s And Children‘s Hospital School Of Molecular & Biomedical Science University Of Adelaide, Australia

El-Yazeed HA (1990) Microbiological studies on fungal skin infection of the skin of different animals and birds. M. V. Sc. thesis. Faculty of Veterinary Medicine, Zagazig University, Egypt

Ezeonu IM, Okafor JI, Ogbonna JC (2011) Laboratory exercises in microbiology. A practical manual for students of tertiary institution, 1st edn. Ephrata Printing and Publishing Company, Nsukka

Fisher MC, Henk DA, Briggs CJ, Brownstein JS, Madoff LC, McCraw SL, Gurr SJ (2012) Emerging fungal threats to animal, plant and ecosystem health. Nature 484(7393):186–194. https://doi.org/10.1038/nature10947 CrossRefPubMed

Halley LD, Standard PG (1973) Laboratory methods in medical mycology, 3rd edn. US Department of Health, Education and Welfare, Center of Disease Control, Atlanta

Hassan MM (2011) Antifungal drug susceptibility of fungi. MVSc thesis. Faculty of Veterinary Medicine, Cairo University, Giza

Hedge J, Wagoner M (2004) Horse conformation: structure, soundness and performance. Globe Pequot 7:111–113

Ibrahim S, Muhammed H (2004) Incidence of ringworm (Tinea capitis) among elementary school children in Gwale and Tarauni local government areas, Kano state, Nigeria. Biol Envir Sci J Tropics 1:25–31

Kabir M, Iliyasu Z, Abubakar IS, Ahmad DZ (2004) Medico-social problems itinerant Almajiris. Niger J Paediatr 32(1):15–18

Khosravi AR, Mahmoudi M (2003) Dermatophytes isolated from domestic animals in Iran. Mycoses 46(5-6):222–225. https://doi.org/10.1046/j.1439-0507.2003.00868.x CrossRefPubMed

Kirby-Bauer AW (1977) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 36:493–496

Moriello KA (2004) Treatment of dermatophytosis in dogs and cats: review of published studies. Vet Dermatol 15(2):99–107. https://doi.org/10.1111/j.1365-3164.2004.00361.x CrossRefPubMed

Norris HA, Elewski BE, Ghannoum MA (1999) Optimal growth conditions for the determination of the antifungal susceptibility of three species of dermatophytes with the use of a microdilution method. J Amer Acad Dermatol 40(6):S9–S13. https://doi.org/10.1016/S0190-9622(99)70392-0 CrossRef

Nwadiaro PO, Ogbonna CIC (1998) The incidence of dermatomycosis among a rural population in Plateau State. Afr J Natural Sci 2:20–21

Nweze EI (2001) Aetiology of Dermatophytosis among school children in north eastern Nigeria. Med Mycol 39:181–184CrossRefPubMed

Nweze EI (2011) Dermatophytosis in domesticated animals. Rev Inst Med Trop Sao Paulo 53(2):95–99. https://doi.org/10.1590/S0036-46652011000200007 CrossRef

Pal M (1987) Dermatophytosis in cattle, clinical and mycological studies. Indian J Anim Sci 57:856–857

Pilsworth RC, Knottenbelt D (2007) Dermatophytosis (ringworm). Equine Vet Ed 19(3):151–154. https://doi.org/10.2746/095777307X186929 CrossRef

Radostits OM, Blood DC, Gay CC (1997) Veterinary medicine: a textbook of diseases of cattle, sheep, pigs, goats and horses, 8th edn. Bailliere Tindall, London

Raymond R, Pihet M (2008) Conventional methods for the diagnosis of dermatophytes. Mycopathologia 166:295–306CrossRef

Santos DA, Barros MES, Hamdan JS (2006) Establishing a method of inoculum preparation for susceptibility testing of Trichophyton rubrum and Trichophyton mentagrophytes. J Clin Microbiol 44(1):98–101. https://doi.org/10.1128/JCM.44.1.98-101.2006 CrossRefPubMedPubMedCentral

Sephavard A, Abdi J, Shirkhani Y, Soleimannejad S (2009) Dermatophytosis in western part of Iran, Khorramabad. Asian J Biol Sci 2:58–65CrossRef

Shah RL, Mall MP, Mohanty GG (1988) Cutaneous candidiasis in Japanese quail (Cortunix cortunix japonica). Mycopathologia 80:33–37CrossRef

Starkey P, Starkey M (2004) Regional and world trends in donkey populations. Anim Tract Netwk East South Africa (ATNESA) 4:312–313

Title: Prevalence and antifungal susceptibility of mycotic agents isolated from skin lesions of horses in Enugu State, Nigeria
Authors: Chioma Inyang Aneke
Iniobong Chukwuebuka Ikenna Ugochukwu
Josephine Okafor
Kennedy Foinkfu Chah
Publication date: 01-05-2018
Publisher: Springer London
Published in: Comparative Clinical Pathology / Issue 3/2018
Print ISSN: 1618-5641
Electronic ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-017-2632-y

At a glance: The STEP trials

Springer Medicine

Prevalence and antifungal susceptibility of mycotic agents isolated from skin lesions of horses in Enugu State, Nigeria

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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