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Clinical & Experimental Metastasis
Published in: Clinical & Experimental Metastasis 1/2014

01-01-2014 | Research Paper

Pre-treatment of mice with tumor-conditioned media accelerates metastasis to lymph nodes and lungs: a new spontaneous breast cancer metastasis model

Authors: Esak Lee, Niranjan B. Pandey, Aleksander S. Popel

Published in: Clinical & Experimental Metastasis | Issue 1/2014

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Abstract

Current spontaneous metastasis models require a long period of observation after establishment of primary tumors to see significant metastatic progression. The degree of metastasis is not consistent among animals: this is problematic since it requires the use of a large number of animals to obtain reliable statistics. Here we report that pre-treatment of animals with tumor-conditioned media (TCM) consistently accelerates spontaneous metastasis in breast cancer. An inguinal breast tumor model facilitated by TCM showed robust anterior metastasis to the axillary and brachial lymph nodes (LN), and the lungs compared to the serum-free media treated group. The LN in TCM-treated animals showed enhanced angiogenesis and lymphangiogenesis. Primary tumors and lungs in TCM-treated animals showed enhanced lymphangiogenesis with no significant change in angiogenesis. TCM-treated animals also showed metastatic dissemination to abdomen from the primary injection site: this would generally enhance metastasis to other organs. In sum, the addition of TCM pre-treatment to current metastasis models results in accelerated and robust metastasis which would enable more efficient evaluation of anti-metastatic agents.
Literature
1.
Maehara Y, Tomisaki S, Oda S et al (1997) Lymph node metastasis and relation to tumor growth potential and local immune response in advanced gastric cancer. Int J Cancer 74(2):224–228PubMedCrossRef
2.
Mori A, Arii S, Furutani M et al (1999) Vascular endothelial growth factor-induced tumor angiogenesis and tumorigenicity in relation to metastasis in a HT1080 human fibrosarcoma cell model. Int J Cancer 80(5):738–743PubMedCrossRef
3.
4.
Petrella BL (2009) Assessment of local proteolytic milieu as a factor in tumor invasiveness and metastasis formation: in vitro collagen degradation and invasion assays. Methods Mol Biol 511:75–84PubMedCrossRef
5.
Carlini MJ, De Lorenzo MS, Puricelli L (2011) Cross-talk between tumor cells and the microenvironment at the metastatic niche. Curr Pharm Biotechnol 12(11):1900–1908PubMedCrossRef
6.
Tammela T, Alitalo K (2010) Lymphangiogenesis: molecular mechanisms and future promise. Cell 140(4):460–476PubMedCrossRef
7.
Alitalo K, Carmeliet P (2002) Molecular mechanisms of lymphangiogenesis in health and disease. Cancer Cell 1(3):219–227PubMedCrossRef
8.
Lee E, Koskimaki JE, Pandey NB et al (2013) Inhibition of lymphangiogenesis and angiogenesis in breast tumor xenografts and lymph nodes by a peptide derived from transmembrane protein 45A. Neoplasia 15(2):112–124PubMedCentralPubMed
9.
Hirakawa S, Kodama S, Kunstfeld R et al (2005) VEGF-A induces tumor and sentinel lymph node lymphangiogenesis and promotes lymphatic metastasis. J Exp Med 201(7):1089–1099PubMedCentralPubMedCrossRef
10.
Wong CC, Zhang H, Gilkes DM et al (2012) Inhibitors of hypoxia-inducible factor 1 block breast cancer metastatic niche formation and lung metastasis. J Mol Med (Berl) 90(7):803–815CrossRef
11.
12.
Yamamoto M, Kikuchi H, Ohta M et al (2008) TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche. Cancer Res 68(23):9754–9762PubMedCrossRef
13.
Kim IS, Baek SH (2010) Mouse models for breast cancer metastasis. Biochem Biophys Res Commun 394(3):443–447PubMedCrossRef
14.
Francia G, Cruz-Munoz W, Man S et al (2011) Mouse models of advanced spontaneous metastasis for experimental therapeutics. Nat Rev Cancer 11(2):135–141PubMedCrossRef
15.
Jenkins DE, Hornig YS, Oei Y et al (2005) Bioluminescent human breast cancer cell lines that permit rapid and sensitive in vivo detection of mammary tumors and multiple metastases in immune deficient mice. Breast Cancer Res 7(4):R444–R454PubMedCentralPubMedCrossRef
16.
17.
Padera TP, Kadambi A, di Tomaso E et al (2002) Lymphatic metastasis in the absence of functional intratumor lymphatics. Science 296(5574):1883–1886PubMedCrossRef
18.
Hayashida T, Takahashi F, Chiba N et al (2010) HOXB9, a gene overexpressed in breast cancer, promotes tumorigenicity and lung metastasis. Proc Natl Acad Sci USA 107(3):1100–1105PubMedCrossRef
19.
Paget S (1989) The distribution of secondary growths in cancer of the breast. 1889. Cancer Metastasis Rev 8(2):98–101PubMed
20.
Hood JL, San RS, Wickline SA (2011) Exosomes released by melanoma cells prepare sentinel lymph nodes for tumor metastasis. Cancer Res 71(11):3792–3801PubMedCrossRef
21.
Alderton GK (2012) Metastasis. Exosomes drive premetastatic niche formation. Nat Rev Cancer 12(7):447PubMedCrossRef
22.
Shih YJ, Hsu KF, Yu JC et al (2012) Synchronous hepatocellular carcinoma and sigmoid colon metastasis presenting as liver and intra-abdominal abscesses. Acta Gastroenterol Belg 75(2):278–279PubMed
23.
Rouhanimanesh Y, Vanderstighelen Y, Vanderputte S et al (2001) Intra-abdominal metastases from primary carcinoma of the lung. Acta Chir Belg 101(6):300–303PubMed
24.
Wason D, Richkind KE (1992) The use of giant cell tumor conditioned media in cytogenetic studies of hematologic malignancies. Cancer Genet Cytogenet 61(2):126–130PubMedCrossRef
25.
Festuccia C, Bologna M, Gravina GL et al (1999) Osteoblast conditioned media contain TGF-beta1 and modulate the migration of prostate tumor cells and their interactions with extracellular matrix components. Int J Cancer 81(3):395–403PubMedCrossRef
26.
Michielsen AJ, O’Sullivan JN, Ryan EJ (2012) Tumor conditioned media from colorectal cancer patients inhibits dendritic cell maturation. Oncoimmunology 1(5):751–753PubMedCentralPubMedCrossRef
27.
Burgess LC, Hall JO (2001) Conditioned media from solid tumor cell lines treated with retinoic acids both decreases and increases proliferation of capillary endothelial cells. Life Sci 69(24):2819–2831PubMedCrossRef
28.
Kaplan RN, Riba RD, Zacharoulis S et al (2005) VEGFR1-positive haematopoietic bone marrow progenitors initiate the pre-metastatic niche. Nature 438(7069):820–827PubMedCentralPubMedCrossRef
29.
Mayorca-Guiliani AE, Yano H, Nakashiro K et al (2012) Premetastatic vasculogenesis in oral squamous cell carcinoma xenograft-draining lymph nodes. Oral Oncol 48(8):663–670PubMedCrossRef
30.
Guerin E, Man S, Xu P et al (2013) A model of postsurgical advanced metastatic breast cancer more accurately replicates the clinical efficacy of antiangiogenic drugs. Cancer Res 73(9):2743–2748PubMedCrossRef
Metadata
Title
Pre-treatment of mice with tumor-conditioned media accelerates metastasis to lymph nodes and lungs: a new spontaneous breast cancer metastasis model
Authors
Esak Lee
Niranjan B. Pandey
Aleksander S. Popel
Publication date
01-01-2014
Publisher
Springer Netherlands
Published in
Clinical & Experimental Metastasis / Issue 1/2014
Print ISSN: 0262-0898
Electronic ISSN: 1573-7276
DOI
https://doi.org/10.1007/s10585-013-9610-9

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