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01-05-2017 | Original Article

Post-absorptive muscle protein turnover affects resistance training hypertrophy

Authors: Paul T. Reidy, Michael S. Borack, Melissa M. Markofski, Jared M. Dickinson, Christopher S. Fry, Rachel R. Deer, Elena Volpi, Blake B. Rasmussen

Published in: European Journal of Applied Physiology | Issue 5/2017

Abstract

Purpose

Acute bouts of resistance exercise and subsequent training alters protein turnover in skeletal muscle. The mechanisms responsible for the changes in basal post-absorptive protein turnover and its impact on muscle hypertrophy following resistance exercise training are unknown. Our goal was to determine whether post-absorptive muscle protein turnover following 12 weeks of resistance exercise training (RET) plays a role in muscle hypertrophy. In addition, we were interested in determining potential molecular mechanisms responsible for altering post-training muscle protein turnover.

Methods

Healthy young men (n = 31) participated in supervised whole body progressive RET at 60–80% 1 repetition maximum (1-RM), 3 days/week for 3 months. Pre- and post-training vastus lateralis muscle biopsies and blood samples taken during an infusion of ¹³C₆ and ¹⁵N phenylalanine and were used to assess skeletal muscle protein turnover in the post-absorptive state. Lean body mass (LBM), muscle strength (determined by dynamometry), vastus lateralis muscle thickness (MT), myofiber type-specific cross-sectional area (CSA), and mRNA were assessed pre- and post-RET.

Results

RET increased strength (12–40%), LBM (~5%), MT (~15%) and myofiber CSA (~20%) (p < 0.05). Muscle protein synthesis (MPS) increased 24% while muscle protein breakdown (MPB) decreased 21%, respectively. These changes in protein turnover resulted in an improved net muscle protein balance in the basal state following RET. Further, the change in basal MPS is positively associated (r = 0.555, p = 0.003) with the change in muscle thickness.

Conclusion

Post-absorptive muscle protein turnover is associated with muscle hypertrophy during resistance exercise training.

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Title: Post-absorptive muscle protein turnover affects resistance training hypertrophy
Authors: Paul T. Reidy
Michael S. Borack
Melissa M. Markofski
Jared M. Dickinson
Christopher S. Fry
Rachel R. Deer
Elena Volpi
Blake B. Rasmussen
Publication date: 01-05-2017
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Applied Physiology / Issue 5/2017
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-017-3566-4

At a glance: The STEP trials

Springer Medicine

Post-absorptive muscle protein turnover affects resistance training hypertrophy

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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