Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

Keynote webinar | Spotlight on medication adherence

LIVE: Thursday 27th June 2024, 18:00-19:30 (CEST)
Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.
ADVANCED SEARCH
Log in
Top
Archives of Virology
Published in: Archives of Virology 3/2018

01-03-2018 | Original Article

Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells

Authors: Yuanxing Gu, Yingshan Zhou, Xinfeng Shi, Yongping Xin, Ying Shan, Cong Chen, Tong Cao, Weihuan Fang, Xiaoliang Li

Published in: Archives of Virology | Issue 3/2018

Login to get access

Abstract

Autophagy is a homeostatic process that has been shown to be vital in the innate immune defense against pathogens. However, little is known about the regulatory role of autophagy in porcine teschovirus 2 (PTV-2) replication. In this study, we found that PTV-2 infection induces a strong increase in GFP-LC3 punctae and endogenous LC3 lipidation. However, PTV-2 infection did not enhance autophagic protein degradation. When cellular autophagy was pharmacologically inhibited by wortmannin or 3-methyladenine, PTV-2 replication increased. The increase in virus yield via autophagy inhibition was further confirmed by silencing atg5, which is required for autophagy. Furthermore, PTV-2 replication was suppressed when autophagy was activated by rapamycin. Together, the results suggest that PTV-2 infection activates incomplete autophagy and that autophagy then inhibits further PTV-2 replication.
Appendix
Available only for authorised users
Literature
1.
Knowles NJ, Hovi T, Hyypiä T (2012) Family Picornaviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Classification and nomenclature of viruses: ninth report of the international committee on taxonomy of viruses. Elsevier, San Diego, pp 855–880
2.
Chiu SC, Hu SC, Chang CC, Chang CY, Huang CC, Pang VF, Wang FI (2012) The role of porcine teschovirus in causing diseases in endemically infected pigs. Vet Microbiol 161:88–95CrossRefPubMed
3.
Tsai ATH, Kuo CC, Kuo YC, Yang JL, Chang CY, Wang FI (2016) The urinary shedding of porcine teschovirus in endemic field situations. Vet Microbiol 182:150–155CrossRefPubMed
4.
Chiu SC, Yang CL, Chen YM, Hu SC, Chiu KC, Lin YC, Chang CY, Wang FI (2014) Multiple models of porcine teschovirus pathogenesis in endemically infected pigs. Vet Microbiol 168:69–77CrossRefPubMed
5.
Jones TH, Muehlhauser V (2017) F-coliphages, porcine adenovirus and porcine teschovirus as potential indicator viruses of fecal contamination for pork carcass processing. Int J Food Microbiol 241:237–243CrossRefPubMed
6.
Wang B, Tian Z-J, Gong D-Q, Li D-Y, Wang Y, Chen J-Z, An T-Q, Peng J-M, Tong G-Z (2010) Isolation of serotype 2 porcine teschovirus in China: evidence of natural recombination. Vet Microbiol 146:138–143CrossRefPubMed
7.
8.
Orvedahl A, MacPherson S, Sumpter R, Tallóczy Z, Zou Z, Levine B (2010) Autophagy protects against Sindbis virus infection of the central nervous system. Cell Host Microbe 7:115–127CrossRefPubMedPubMedCentral
9.
Orvedahl A, Alexander D, Tallóczy Z, Sun Q, Wei Y, Zhang W, Burns D, Leib DA, Levine B (2007) HSV-1 ICP34. 5 confers neurovirulence by targeting the Beclin 1 autophagy protein. Cell Host Microbe 1:23–35CrossRefPubMed
10.
11.
12.
Zhu B, Xu F, Li J, Shuai J, Li X, Fang W (2012) Porcine circovirus type 2 explores the autophagic machinery for replication in PK-15 cells. Virus Res 163:476–485CrossRefPubMed
13.
Hu B, Zhang Y, Jia L, Wu H, Fan C, Sun Y, Ye C, Liao M, Zhou J (2015) Binding of the pathogen receptor HSP90AA1 to avibirnavirus VP2 induces autophagy by inactivating the AKT-MTOR pathway. Autophagy 11:503–515CrossRefPubMedPubMedCentral
14.
Levine B, Klionsky DJ (2017) Autophagy wins the 2016 Nobel Prize in Physiology or Medicine: Breakthroughs in baker’s yeast fuel advances in biomedical research. Proc Natl Acad Sci USA 114:201–205CrossRefPubMed
15.
Shibutani ST, Saitoh T, Nowag H, Münz C, Yoshimori T (2015) Autophagy and autophagy-related proteins in the immune system. Nat Immunol 16:1014CrossRefPubMed
16.
17.
Cheng J-H, Sun Y-J, Zhang F-Q, Zhang X-R, Qiu X-S, Yu L-P, Wu Y-T, Ding C (2016) Newcastle disease virus NP and P proteins induce autophagy via the endoplasmic reticulum stress-related unfolded protein response. Sci Rep 6:24721CrossRefPubMedPubMedCentral
18.
Sun Y, Yu S, Ding N, Meng C, Meng S, Zhang S, Zhan Y, Qiu X, Tan L, Chen H, Song C, Ding C (2014) Autophagy Benefits the Replication of Newcastle Disease Virus in Chicken Cells and Tissues. J Virol 88:525–537CrossRefPubMedPubMedCentral
19.
Green AM, Beatty PR, Hadjilaou A, Harris E (2014) Innate immunity to dengue virus infection and subversion of antiviral responses. J Mol Biol 426:1148–1160CrossRefPubMed
20.
Jin R, Zhu W, Cao S, Chen R, Jin H, Liu Y, Wang S, Wang W, Xiao G (2013) Japanese encephalitis virus activates autophagy as a viral immune evasion strategy. PLoS One 8:e52909CrossRefPubMedPubMedCentral
21.
Li J-K, Liang J-J, Liao C-L, Lin Y-L (2012) Autophagy is involved in the early step of Japanese encephalitis virus infection. Microbes Infect 14:159–168CrossRefPubMed
22.
Chan ST, Lee J, Narula M, Ou J-HJ (2016) Suppression of Host Innate Immune Response by Hepatitis C Virus via Induction of Autophagic Degradation of TRAF6. J Virol 90:10928–10935CrossRefPubMedPubMedCentral
23.
Dreux M, Chisari F (2009) Autophagy proteins promote hepalitis C virus replication. Autophagy 5:1224–1225CrossRefPubMed
24.
Tallóczy Z, Virgin I, Herbert Levine B (2006) PKR-dependent xenophagic degradation of herpes simplex virus type 1. Autophagy 2:24–29CrossRefPubMed
25.
Liu Y, Schiff M, Czymmek K, Tallóczy Z, Levine B, Dinesh-Kumar S (2005) Autophagy regulates programmed cell death during the plant innate immune response. Cell 121:567–577CrossRefPubMed
26.
Mateo R, Nagamine CM, Spagnolo J, Méndez E, Rahe M, Gale M, Yuan J, Kirkegaard K (2013) Inhibition of cellular autophagy deranges dengue virion maturation. J Virol 87:1312–1321CrossRefPubMedPubMedCentral
27.
28.
Huang SC, Chang CL, Wang PS, Tsai Y, Liu HS (2009) Enterovirus 71-induced autophagy detected in vitro and in vivo promotes viral replication. J Med Virol 81:1241–1252CrossRefPubMed
29.
O’donnell V, Pacheco JM, LaRocco M, Burrage T, Jackson W, Rodriguez LL, Borca MV, Baxt B (2011) Foot-and-mouth disease virus utilizes an autophagic pathway during viral replication. Virology 410:142–150CrossRefPubMed
30.
Berryman S, Brooks E, Burman A, Hawes P, Roberts R, Netherton C, Monaghan P, Whelband M, Cottam E, Elazar Z (2012) Foot-and-mouth disease virus induces autophagosomes during cell entry via a class III phosphatidylinositol 3-kinase-independent pathway. J Virol 86:12940–12953CrossRefPubMedPubMedCentral
31.
Zhang Y, Li Z, Ge X, Guo X, Yang H (2011) Autophagy promotes the replication of encephalomyocarditis virus in host cells. Autophagy 7:613–628CrossRefPubMed
32.
33.
Quiner CA, Jackson WT (2010) Fragmentation of the Golgi apparatus provides replication membranes for human rhinovirus 1A. Virology 407:185–195CrossRefPubMed
34.
Delorme-Axford E, Morosky S, Bomberger J, Stolz DB, Jackson WT, Coyne CB (2014) BPIFB3 regulates autophagy and coxsackievirus B replication through a noncanonical pathway independent of the core initiation machinery. MBio 5:e02114–e02147CrossRef
35.
Taylor MP, Kirkegaard K (2008) Potential subversion of autophagosomal pathway by picornaviruses. Autophagy 4:286–289CrossRefPubMed
36.
Münz C (2016) Autophagy beyond intracellular MHC class II antigen presentation. Trends Immunol 37:755–763CrossRefPubMed
37.
Pankiv S, Clausen TH, Lamark T, Brech A, Bruun J-A, Outzen H, Øvervatn A, Bjørkøy G, Johansen T (2007) p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy. J Biol Chem 282:24131–24145CrossRefPubMed
38.
39.
Gladue D, O’donnell V, Baker-Branstetter R, Holinka L, Pacheco J, Fernandez-Sainz I, Lu Z, Brocchi E, Baxt B, Piccone M (2012) Foot-and-mouth disease virus nonstructural protein 2C interacts with Beclin1, modulating virus replication. J Virol 86:12080–12090CrossRefPubMedPubMedCentral
40.
41.
Jackson WT, Giddings TH Jr, Taylor MP, Mulinyawe S, Rabinovitch M, Kopito RR, Kirkegaard K (2005) Subversion of cellular autophagosomal machinery by RNA viruses. PLoS Biol 3:e156CrossRefPubMedPubMedCentral
42.
Cherry S, Kunte A, Wang H, Coyne C, Rawson RB, Perrimon N (2006) COPI activity coupled with fatty acid biosynthesis is required for viral replication. PLoS Pathog 2:e102CrossRefPubMedPubMedCentral
43.
Levine B, Deretic V (2007) Unveiling the roles of autophagy in innate and adaptive immunity. Nat Rev Immunol 7:767–777CrossRefPubMed
44.
Nardacci R, Ciccosanti F, Marsella C, Ippolito G, Piacentini M, Fimia GM (2017) Role of autophagy in HIV infection and pathogenesis. J Intern Med 281:422–432CrossRefPubMed
45.
Shoji-Kawata S, Sumpter R Jr, Leveno M, Campbell GR, Zou Z, Kinch L, Wilkins AD, Sun Q, Pallauf K, MacDuff D (2013) Identification of a candidate therapeutic autophagy–inducing peptide. Nature 494:201CrossRefPubMedPubMedCentral
Metadata
Title
Porcine teschovirus 2 induces an incomplete autophagic response in PK-15 cells
Authors
Yuanxing Gu
Yingshan Zhou
Xinfeng Shi
Yongping Xin
Ying Shan
Cong Chen
Tong Cao
Weihuan Fang
Xiaoliang Li
Publication date
01-03-2018
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 3/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-017-3652-2

Other articles of this Issue 3/2018

Archives of Virology 3/2018 Go to the issue

Review

Pharmacophoric characteristics of dengue virus NS2B/NS3pro inhibitors: a systematic review of the most promising compounds

Annotated Sequence Record

Complete genome sequence of Paris mosaic necrosis virus, a distinct member of the genus Potyvirus

Correction

Correction to: Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR

Annotated Sequence Record

Complete genome sequence of a new strain of Lagenaria siceraria endornavirus from China

Annotated Sequence Record

Completed sequence and corrected annotation of the genome of maize Iranian mosaic virus

Original Article

Detection of hepatitis B virus DNA and HBsAg from postmortem blood and bloodstains
Live Webinar | 27-06-2024 | 18:00 (CEST)

Keynote webinar | Spotlight on medication adherence

Reserve your place

Live: Thursday 27th June 2024, 18:00-19:30 (CEST)

WHO estimates that half of all patients worldwide are non-adherent to their prescribed medication. The consequences of poor adherence can be catastrophic, on both the individual and population level.

Join our expert panel to discover why you need to understand the drivers of non-adherence in your patients, and how you can optimize medication adherence in your clinics to drastically improve patient outcomes.

Prof. Kevin Dolgin
Prof. Florian Limbourg
Prof. Anoop Chauhan
Developed by: Springer Medicine
Obesity Clinical Trial Summary

At a glance: The STEP trials

Read more

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.

Developed by: Springer Medicine

Highlights from the ACC 2024 Congress

Year in Review: Pediatric cardiology

Pediatric Cardiology Video

Watch Dr. Anne Marie Valente present the last year's highlights in pediatric and congenital heart disease in the official ACC.24 Year in Review session.

Year in Review: Pulmonary vascular disease

Cardiopulmonary Comorbidity Video

The last year's highlights in pulmonary vascular disease are presented by Dr. Jane Leopold in this official video from ACC.24.

Year in Review: Valvular heart disease

Valvular Heart Disease Video

Watch Prof. William Zoghbi present the last year's highlights in valvular heart disease from the official ACC.24 Year in Review session.

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Heart Failure Video

Watch this official video from ACC.24. Dr. Biykem Bozkurt discusses last year's major advances in heart failure and cardiomyopathies.

Watch now

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

16-04-2024 Type 2 Diabetes News

Incentivised to exercise

11-04-2024 Lifestyle Modifications News

New intervention for STEMI-related cardiogenic shock

10-04-2024 Myocardial Infarction News

» View more highlights on the ACC 2024 congress hub page

Image Credits