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Published in: Archives of Virology 10/2018

01-10-2018 | Brief Report

Phylogenetic analysis of porcine reproductive and respiratory syndrome virus isolates from Northern Ireland

Authors: Natalie Smith, Ultan F. Power, John McKillen

Published in: Archives of Virology | Issue 10/2018

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Abstract

To investigate the genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) in Northern Ireland, the ORF5 gene from nine field isolates was sequenced and phylogenetically analysed. The results revealed relatively high diversity amongst isolates, with 87.6-92.2% identity between farms at the nucleotide level and 84.1-93.5% identity at the protein level. Phylogenetic analysis confirmed that all nine isolates belonged to the European (type 1) genotype and formed a cluster within the subtype 1 subgroup. This study provides the first report on PRRSV isolate diversity in Northern Ireland.
Literature
2.
go back to reference Keffaber KK (1989) Reproductive failure of unknown etiology. Am Assoc Swine Pract Newsl 1(2):1–9 Keffaber KK (1989) Reproductive failure of unknown etiology. Am Assoc Swine Pract Newsl 1(2):1–9
3.
go back to reference Wensvoort G, Terpstra C, Pol JM et al (1991) Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Vet Q 13:121–130CrossRefPubMed Wensvoort G, Terpstra C, Pol JM et al (1991) Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Vet Q 13:121–130CrossRefPubMed
4.
go back to reference Allende R, Lewis TL, Lu Z et al (1999) North American and European porcine reproductive and respiratory syndrome viruses differ in non-structural protein coding regions. Gen Virol 80:307–315CrossRef Allende R, Lewis TL, Lu Z et al (1999) North American and European porcine reproductive and respiratory syndrome viruses differ in non-structural protein coding regions. Gen Virol 80:307–315CrossRef
5.
go back to reference Meng X (2000) Heterogeneity of porcine reproductive and respiratory syndrome virus: implications for current vaccine efficacy and future vaccine development. Vet Microbiol 74:309–329CrossRefPubMed Meng X (2000) Heterogeneity of porcine reproductive and respiratory syndrome virus: implications for current vaccine efficacy and future vaccine development. Vet Microbiol 74:309–329CrossRefPubMed
6.
go back to reference Fang Y, Schneider P, Zhang WP et al (2007) Diversity and evolution of a newly emerged North American Type 1 porcine arterivirus: analysis of isolates collected between 1999 and 2004. Arch Virol 152:1009–1017CrossRefPubMed Fang Y, Schneider P, Zhang WP et al (2007) Diversity and evolution of a newly emerged North American Type 1 porcine arterivirus: analysis of isolates collected between 1999 and 2004. Arch Virol 152:1009–1017CrossRefPubMed
8.
go back to reference Stadejek T, Stankevicius A, Murtaugh MP, Oleksiewicz MB (2013) Molecular evolution of PRRSV in Europe: current state of play. Vet Microbiol 165:21–28CrossRefPubMed Stadejek T, Stankevicius A, Murtaugh MP, Oleksiewicz MB (2013) Molecular evolution of PRRSV in Europe: current state of play. Vet Microbiol 165:21–28CrossRefPubMed
9.
go back to reference Brar MS, Shi M, Murtaugh MP, Leung FCC (2015) Evolutionary diversification of type 2 porcine reproductive and respiratory syndrome virus. J Gen Virol 96:1570–1580CrossRefPubMed Brar MS, Shi M, Murtaugh MP, Leung FCC (2015) Evolutionary diversification of type 2 porcine reproductive and respiratory syndrome virus. J Gen Virol 96:1570–1580CrossRefPubMed
10.
go back to reference Marozin S, Gregory V, Cameron K et al (2002) Antigenic and genetic diversity among swine influenza viruses in Europe. J Gen Virol 83:735–745CrossRefPubMed Marozin S, Gregory V, Cameron K et al (2002) Antigenic and genetic diversity among swine influenza viruses in Europe. J Gen Virol 83:735–745CrossRefPubMed
11.
go back to reference Midgley SE, Banyai K, Buesa J et al (2012) Diversity and zoonotic potential of rotaviruses in swine and cattle across Europe. Vet Microbiol 156:238–245CrossRefPubMed Midgley SE, Banyai K, Buesa J et al (2012) Diversity and zoonotic potential of rotaviruses in swine and cattle across Europe. Vet Microbiol 156:238–245CrossRefPubMed
12.
go back to reference Gunn L, Collins PJ, O’Connell MJ, O’Shea H (2015) Phylogenetic investigation of enteric bovine coronavirus in Ireland reveals partitioning between European and global strains. Ir Vet J 68:31CrossRefPubMedPubMedCentral Gunn L, Collins PJ, O’Connell MJ, O’Shea H (2015) Phylogenetic investigation of enteric bovine coronavirus in Ireland reveals partitioning between European and global strains. Ir Vet J 68:31CrossRefPubMedPubMedCentral
13.
go back to reference Pesente P, Rebonato V, Sandri G et al (2006) Phylogenetic analysis of ORF5 and ORF7 sequences of porcine reproductive and respiratory syndrome virus (PRRSV) from PRRS-positive Italian farms: a showcase for PRRSV epidemiology and its consequences on farm management. Vet Microbiol 114:214–224CrossRefPubMed Pesente P, Rebonato V, Sandri G et al (2006) Phylogenetic analysis of ORF5 and ORF7 sequences of porcine reproductive and respiratory syndrome virus (PRRSV) from PRRS-positive Italian farms: a showcase for PRRSV epidemiology and its consequences on farm management. Vet Microbiol 114:214–224CrossRefPubMed
14.
go back to reference Kearse M, Moir R, Wilson A et al (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649CrossRefPubMedPubMedCentral Kearse M, Moir R, Wilson A et al (2012) Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28:1647–1649CrossRefPubMedPubMedCentral
15.
go back to reference Saitou N, Nei M (1987) The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMed Saitou N, Nei M (1987) The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMed
17.
go back to reference Wei ZZ, Lin T, Sun LC et al (2012) N-linked glycosylation of GP5 of porcine reproductive and respiratory syndrome virus is critically important for virus replication in vivo. J Virol 86:9941–9951CrossRefPubMedPubMedCentral Wei ZZ, Lin T, Sun LC et al (2012) N-linked glycosylation of GP5 of porcine reproductive and respiratory syndrome virus is critically important for virus replication in vivo. J Virol 86:9941–9951CrossRefPubMedPubMedCentral
18.
go back to reference Ostrowski M, Galeota JA, Jar AM et al (2002) Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP. J Virol 76:4241–4250CrossRefPubMedPubMedCentral Ostrowski M, Galeota JA, Jar AM et al (2002) Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP. J Virol 76:4241–4250CrossRefPubMedPubMedCentral
19.
go back to reference Wissink EHJ, van Wijk HAR, Kroese MV et al (2003) The major envelope protein, GP5, of a European porcine reproductive and respiratory syndrome virus contains a neutralization epitope in its N-terminal ectodomain. J Gen Virol 84:1535–1543CrossRefPubMed Wissink EHJ, van Wijk HAR, Kroese MV et al (2003) The major envelope protein, GP5, of a European porcine reproductive and respiratory syndrome virus contains a neutralization epitope in its N-terminal ectodomain. J Gen Virol 84:1535–1543CrossRefPubMed
20.
go back to reference Ropp SL, Wees CEM, Fang Y et al (2004) Characterization of emerging European-like porcine reproductive and respiratory syndrome virus isolates in the United States. J Virol 78:3684–3703CrossRefPubMedPubMedCentral Ropp SL, Wees CEM, Fang Y et al (2004) Characterization of emerging European-like porcine reproductive and respiratory syndrome virus isolates in the United States. J Virol 78:3684–3703CrossRefPubMedPubMedCentral
21.
go back to reference Meng XJ, Paul PS, Halbur PG, Morozov I (1995) Sequence comparison of open reading frames 2–5 of low and high virulence United States isolates of porcine reproductive and respiratory syndrome virus. J Gen Virol 76:3181–3188CrossRefPubMed Meng XJ, Paul PS, Halbur PG, Morozov I (1995) Sequence comparison of open reading frames 2–5 of low and high virulence United States isolates of porcine reproductive and respiratory syndrome virus. J Gen Virol 76:3181–3188CrossRefPubMed
22.
go back to reference Weiland E, Wieczorek-Krohmer M, Kohl D et al (1999) Monoclonal antibodies to the GP5 of porcine reproductive and respiratory syndrome virus are more effective in virus neutralization than monoclonal antibodies to the GP4. Vet Microbiol 66:171–186CrossRefPubMed Weiland E, Wieczorek-Krohmer M, Kohl D et al (1999) Monoclonal antibodies to the GP5 of porcine reproductive and respiratory syndrome virus are more effective in virus neutralization than monoclonal antibodies to the GP4. Vet Microbiol 66:171–186CrossRefPubMed
23.
go back to reference Frossard J-P, Hughes GJ, Westcott DG et al (2013) Porcine reproductive and respiratory syndrome virus: genetic diversity of recent British isolates. Vet Microbiol 162:507–518CrossRefPubMed Frossard J-P, Hughes GJ, Westcott DG et al (2013) Porcine reproductive and respiratory syndrome virus: genetic diversity of recent British isolates. Vet Microbiol 162:507–518CrossRefPubMed
24.
go back to reference Kvisgaard LK, Hjulsager CK, Kristensen CS et al (2013) Genetic and antigenic characterization of complete genomes of type 1 porcine reproductive and respiratory syndrome viruses (PRRSV) isolated in Denmark over a period of 10 years. Virus Res 178:197–205CrossRefPubMed Kvisgaard LK, Hjulsager CK, Kristensen CS et al (2013) Genetic and antigenic characterization of complete genomes of type 1 porcine reproductive and respiratory syndrome viruses (PRRSV) isolated in Denmark over a period of 10 years. Virus Res 178:197–205CrossRefPubMed
25.
go back to reference Li Y, Wang X, Jiang P et al (2009) Genetic variation analysis of porcine reproductive and respiratory syndrome virus isolated in China from 2002 to 2007 based on ORF5. Vet Microbiol 138:150–155CrossRefPubMed Li Y, Wang X, Jiang P et al (2009) Genetic variation analysis of porcine reproductive and respiratory syndrome virus isolated in China from 2002 to 2007 based on ORF5. Vet Microbiol 138:150–155CrossRefPubMed
26.
go back to reference Stadejek T, Oleksiewicz MB, Scherbakov AV et al (2008) Definition of subtypes in the European genotype of porcine reproductive and respiratory syndrome virus: nucleocapsid characteristics and geographical distribution in Europe. Arch Virol 153:1479–1488CrossRefPubMed Stadejek T, Oleksiewicz MB, Scherbakov AV et al (2008) Definition of subtypes in the European genotype of porcine reproductive and respiratory syndrome virus: nucleocapsid characteristics and geographical distribution in Europe. Arch Virol 153:1479–1488CrossRefPubMed
27.
go back to reference Renukaradhya GJ, Meng X-J, Calvert JG et al (2015) Live porcine reproductive and respiratory syndrome virus vaccines: current status and future direction. Vaccine 33:4069–4080CrossRefPubMed Renukaradhya GJ, Meng X-J, Calvert JG et al (2015) Live porcine reproductive and respiratory syndrome virus vaccines: current status and future direction. Vaccine 33:4069–4080CrossRefPubMed
28.
go back to reference Charerntantanakul W (2012) Porcine reproductive and respiratory syndrome virus vaccines: immunogenicity, efficacy and safety aspects. World J Virol 1:23CrossRefPubMedPubMedCentral Charerntantanakul W (2012) Porcine reproductive and respiratory syndrome virus vaccines: immunogenicity, efficacy and safety aspects. World J Virol 1:23CrossRefPubMedPubMedCentral
29.
go back to reference Trus I, Bonckaert C, van der Meulen K, Nauwynck HJ (2014) Efficacy of an attenuated European subtype 1 porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in pigs upon challenge with the East European subtype 3 PRRSV strain lena. Vaccine 32:2995–3003CrossRefPubMed Trus I, Bonckaert C, van der Meulen K, Nauwynck HJ (2014) Efficacy of an attenuated European subtype 1 porcine reproductive and respiratory syndrome virus (PRRSV) vaccine in pigs upon challenge with the East European subtype 3 PRRSV strain lena. Vaccine 32:2995–3003CrossRefPubMed
30.
go back to reference Il Kim W, Yoon KJ (2008) Molecular assessment of the role of envelope-associated structural proteins in cross neutralization among different PRRS viruses. Virus Genes 37:380–391CrossRef Il Kim W, Yoon KJ (2008) Molecular assessment of the role of envelope-associated structural proteins in cross neutralization among different PRRS viruses. Virus Genes 37:380–391CrossRef
31.
go back to reference Plagemann PGW, Rowland RRR, Faaberg KS (2002) The primary neutralization epitope of porcine respiratory and reproductive syndrome virus strain VR-2332 is located in the middle of the GP5 ectodomain. Arch Virol 147:2327–2347CrossRefPubMed Plagemann PGW, Rowland RRR, Faaberg KS (2002) The primary neutralization epitope of porcine respiratory and reproductive syndrome virus strain VR-2332 is located in the middle of the GP5 ectodomain. Arch Virol 147:2327–2347CrossRefPubMed
32.
go back to reference Faaberg KS, Hocker JD, Erdman MM et al (2006) Neutralizing antibody responses of pigs infected with natural GP5 N-glycan mutants of porcine reproductive and respiratory syndrome virus. Viral Immunol 19:294–304CrossRefPubMed Faaberg KS, Hocker JD, Erdman MM et al (2006) Neutralizing antibody responses of pigs infected with natural GP5 N-glycan mutants of porcine reproductive and respiratory syndrome virus. Viral Immunol 19:294–304CrossRefPubMed
33.
go back to reference Ansari IH, Kwon B, Osorio FA, Pattnaik AK (2006) Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity, antigenicity, and ability to induce neutralizing antibodies. J Virol 80:3994–4004CrossRefPubMedPubMedCentral Ansari IH, Kwon B, Osorio FA, Pattnaik AK (2006) Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity, antigenicity, and ability to induce neutralizing antibodies. J Virol 80:3994–4004CrossRefPubMedPubMedCentral
34.
go back to reference Vu HLX, Kwon B, Yoon K-J et al (2011) Immune evasion of porcine reproductive and respiratory syndrome virus through glycan shielding involves both glycoprotein 5 as well as glycoprotein 3. J Virol 85:5555–5564CrossRefPubMedPubMedCentral Vu HLX, Kwon B, Yoon K-J et al (2011) Immune evasion of porcine reproductive and respiratory syndrome virus through glycan shielding involves both glycoprotein 5 as well as glycoprotein 3. J Virol 85:5555–5564CrossRefPubMedPubMedCentral
Metadata
Title
Phylogenetic analysis of porcine reproductive and respiratory syndrome virus isolates from Northern Ireland
Authors
Natalie Smith
Ultan F. Power
John McKillen
Publication date
01-10-2018
Publisher
Springer Vienna
Published in
Archives of Virology / Issue 10/2018
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-018-3886-7

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